We narrowed to 14,372 results for: hal
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Plasmid#97015PurposeExpressing Pseudomonas aeruginosa orf PA4511 proteinDepositorInsertP. aeruginosa orf PA4511
UseTagsN-ter TEV protease cleavable 6HIsExpressionMutationnonePromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSATAG
Plasmid#97004PurposeExpressing MRSA TAG proteinDepositorInsertDNA-3-methyladenine glycosylase I
UseTagsN-ter TEV protease cleavable 6HisExpressionMutationPromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
Cry2 mcherry Gamma 9 in pcDNA3.1
Plasmid#64208PurposemCherry fused between Cry2 and gamma 9 to study cell migrationDepositorUseTagsmcherry and n/aExpressionMammalianMutationPromoterCMVAvailable sinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hHP1β
Plasmid#103829PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
ARHGEF11(DHPH)-CRY2-mCherry
Plasmid#89481PurposeAlso known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11DepositorUseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE
Plasmid#51509PurposeCan be used to generate AAV virus that will express cytoplasmic tdTomato and presynaptic (synaptophysin-fused) EGFP in the presence of Cre in neurons from the synapsin promoterDepositorHas ServiceAAV1InserttdTomato-T2A-SypEGFP
UseAAVTagsExpressionMutationPromoterphSyn1Available sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRL_CT_GI_del1
Plasmid#98744PurposeFor red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.DepositorUseSynthetic BiologyTagsSV40-NLS, VP64-AD, and synTALE-DBDExpressionYeastMutationmutated nucleotide 576 C to A, deleted amino acid…PromoterFBA1 and TDH3Available sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-RCaMP1h
Plasmid#42874Purposea single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRubyDepositorInsertRed Fluorescent Calcium binding Protein
UseTagsEK (Enterokinase) Recognition Site, His Tag (6x),…ExpressionBacterialMutationPromoterT7Available sinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Ezh2[SET]-dCas9
Plasmid#100087PurposeCatalytic domain [SET] of mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expressionDepositorInsertEzh2[SET] (Ezh2 Mouse)
UseCRISPRTags3XFLag-NLS-Ezh2[SET]-dCas9-NLSExpressionMammalianMutationEzh2[SET] includes aa 482-746PromoterCMVAvailable sinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1012 - pAAV EF1a DIO Nuc-eYFP
Plasmid#75082PurposeAn AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.DepositorInsertNuc-eYFP
UseAAV and Cre/LoxTagsNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr15
Plasmid#100537PurposeCEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCreC, CreN, and SV40 NLSExpressionYeastMutationN-terminal version of PhyB (AA 1-621)/ Mutation o…PromoterFBA1 and TDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]
Plasmid#78174PurposeAAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterhuman synapsinAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-hTRF1-tagRFP-T
Plasmid#103811PurposeBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr16
Plasmid#100538PurposeCEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCdc1 NLS, CreC, CreN, and SV40 NLSExpressionYeastMutationAA 1-621 of PhyB/ Mutation nucleotide 576 C-->APromoterFBA1 and TDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-K2-L2
Plasmid#80684PurposeGateway Entry vector containing a temperature-sensitive mouse DHFRDepositorUseSynthetic BiologyTags3xHAExpressionBacterial, Insect, Mammalia…Mutationmouse DHFR with N-terminal UbiquitinPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hHP1β
Plasmid#103830PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC710 - pAAV EF1a DIO Mem-AcGFP
Plasmid#75081PurposeAn AAV packaging vector that expresses Cre-dependent membrane-localized AcGFP under control of the EF1a promoter.DepositorInsertMem-AcGFP
UseAAV and Cre/LoxTagsPalmitylation siteExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSA0482
Plasmid#96968Purposeexpression of methicillin-resistant Staphylococcus aureus orf 0482DepositorInsertMRSA ORF0482
UseTagsN-ter TEV protease cleavable 6HisExpressionBacterialMutationnonePromoterT7Available sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-tdTomato-Raf1
Plasmid#104176PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato and Raf1DepositorUseTagsExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-mCherry-Raf1
Plasmid#104066PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1DepositorUseTagsExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only