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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 3921 - 3940 of 16276 results

  1. pFD115

    Plasmid
    #124768
    Purpose
    pFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vector
    Depositor
    David Bikard
    Publication
    Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Ptet
    cas9
    term PpflB sgRNA (BsaI)
    Use
    CRISPR
    Promoter
    Ptet
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    124768-plasmid-map-sequence-id-246185

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  10. p330 VQR

    Plasmid
    #101730
    Purpose
    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101730-plasmid-map-sequence-id-192072

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  12. K560_AviC_coBirA

    Plasmid
    #129765
    Purpose
    Expresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    Avi-tag GLNDIFEAQKIEWH and His6
    Expression
    Bacterial
    Mutation
    Truncation at 559
    Available since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129765-plasmid-map-sequence-id-263583

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  18. mVenus-Lifeact-7

    Plasmid
    #54688
    Purpose
    Localization: Actin, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    Lifeact-mVenus
    Expression
    Mammalian
    Available since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  20. pTR47m4

    Plasmid
    #104068
    Purpose
    Luciferase Reporter for Formaldehyde Sensing
    Depositor
    David Liu
    Publication
    Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.
    Inserts
    xluxAB
    frmR
    frm Promoter m4
    Use
    Luciferase and Synthetic Biology
    Expression
    Bacterial
    Mutation
    23r and 25th nucleotides in sequence mutated from…
    Available since
    March 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    104068-plasmid-map-sequence-id-205132

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Showing: 3921 - 3940 of 16276 results