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Plasmid#199229PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertAmphiregulin preproprotein (AREG Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
CNNM3-CBS-YPet
Plasmid#149686PurposeFor the FRET-based binding assay between human CNNM3 CBS domain and PRL2.DepositorInsertCNNM3-CBS-YPet (CNNM3 Synthetic, Human)
UseTags8 histidine tagExpressionBacterialMutationPromoterT7Available sinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Bxb1_jRCaMP1b
Plasmid#183758PurposeBxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporterDepositorInsertjRCaMP1b
UseTagsNESExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-2x
Plasmid#172282PurposeExpressing EGFP mRNA fused with 2 tandem repeats of a 50-base sequenceDepositorInsertEGFP-N1-2x
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-4x
Plasmid#172283PurposeExpressing EGFP mRNA fused with 4 tandem repeats of a 50-base sequenceDepositorInsertEGFP-N1-4x
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMV TRE3G BCL2L11 G156E Neo
Plasmid#140748PurposeInducibly expresses G156E mutant BIM in mammalian cellsDepositorInsertBCL2L11 (BCL2L11 Human)
UseLentiviralTagsExpressionMammalianMutationChanged Glycine 156 to GlutamatePromoterCMV TRE3GAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMaKIIalpha-mKO2
Plasmid#86156PurposeLentiviral fluorescent reporter using the neuronal cell type-specific promoter of the CaM Kinase II alpha gene.DepositorInsertmKO2
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
vsv-CenpB 1-166-Sgo1-GFP
Plasmid#108495Purposeexpression of vsv-CenpB-1-166-Sgo1-EGFPDepositorInsertCenpB-Shugoshin 1 (SGO1 Human)
UseTagsEGFP and VSVExpressionMammalianMutationCenpB 1-166 fusion before Sgo1PromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex-Turbo/GFP
Plasmid#197886PurposeCan be used to generate AAV virus that will express Turbo/GFP in the presence of CreDepositorInsertTurbo/GFP
UseAAVTagsExpressionMutationN/APromoterCAGAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA
Plasmid#104984PurposeExpresses ST-Renilla reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBlastR-MCS-QF-MiniWhite
Plasmid#165906PurposeBlasticidin resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF (VE5596)
Plasmid#139768PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette.DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterPH or p10Available sinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
NG1237 pCI-TPI-PTC160-4H
Plasmid#65804PurposeNMD reporter mRNADepositorInsertTPI (TPI1 Human)
UseTags4H (4 copies of short beta-globin 3'UTR for …ExpressionMammalianMutationpremature stop codon at 160PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSN69
Plasmid#100125Purposeexpresses putative cMYC DBD with a HA tagDepositorInsertmCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
UseLentiviralTagsExpressionMammalianMutationInsert corresponds to the DBD of cMYCPromoterAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMB1052:miniTol2-U6-(empty)SaCas9_gRNA-CAG-Cre-P2A-NeoR-WPRE
Plasmid#168108PurposeTol2 transposon vector of empty U6-S.aureus Cas9 gRNA cassette and CAG-driven Cre-NeoRDepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMutationPromoterAvailable sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xFN-Tel-TAL/pMXs-puro
Plasmid#63590PurposeExpresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat. A retroviral expression vector with the puromycin resistance gene.DepositorInsert3xFVN-Tel-TAL
UseRetroviral and TALENTags3xFLAG-tag, V5-tag, and nuclear localization sign…ExpressionMammalianMutationPromoterLTRAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1(-) + SLNCR1 _701-902
Plasmid#86821Purposemammalian expression of lncRNA LINC00673/SLNCR1 deletion constructDepositorInsertLINC00673 (LINC00673 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSHDY*in_PpetE:BCD2:mVenus
Plasmid#133970PurposeHeterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.DepositorInsertmVenus
UseSynthetic BiologyTagsExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;dll4CE2-P1Egfp
Plasmid#90147Purposecontains dll4 enhancer 2 sequence, and dll4 specific gene promoter driving expression of eGFPDepositorInsertdll4E2P1Egfp (dll4 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pG418R-MCS-QF-MiniWhite
Plasmid#165902PurposeG418 resistant QF driver vector with Mini-white CDS eye marker. Contains an enhancer grammar GB20 entry point for custom enhancers. Standard cut-and-paste cloning can also be used. Vector uses Purple-White bacteria colony screening for identification of correct cloningDepositorInsertSelectable Empty Enhancer Driver
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only