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CAG-FLEx(FRT)-G

Plasmid

#67828

Purpose

Flp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cells

Depositor

Liqun Luo

Article

Schwarz et al Nature. 2015 Aug 6;524(7563):88-92. doi:

Insert

rabies glycoprotein

Use

AAV

Expression

Mammalian

Available Since

Sept. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ2214 - Intron GG1 - 900 bp

Plasmid

#159880

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG1 - 900 bp

Expression

Worm

Mutation

Not applicable

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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CROP_C9_Puro_ERBB2

Plasmid

#183287

Purpose

All-in-One CRISPRko system with a guide RNA that targets ERBB2 gene

Depositor

Prashant Mali

Article

Hu et al Biomaterials. 2022 Jan;280:121276. doi:

Insert

ERBB2

Use

Lentiviral

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_hU6

Plasmid

#102840

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Promoter

hU6

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:mNeonGreen-Rab5a

Plasmid

#192355

Purpose

zfRabaa tagged with mNeonGreen, expressed under the UAS promoter

Depositor

Francesca Peri

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

Rab5a (rab5aa Zebrafish)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-EA-BE4max-NG-BlastR

Plasmid

#152992

Purpose

High purity C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4 H47E+S48A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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GFAP-Cre

Plasmid

#24704

Depositor

Michael Sofroniew

Article

Bush et al Neuron. 1999 Jun . 23(2):297-308.

Insert

Cre

Expression

Mammalian

Promoter

GFAP (glial fibrillary acidic protein)

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only

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MBP-CRY1-PHR

Plasmid

#26035

Depositor

Johann Deisenhofer

Article

Brautigam et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

CRY1 (CRY1 Mustard Weed)

Tags

MBP

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
mCherry-Vinculin-N-21

Plasmid

#55160

Purpose

Localization: Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Burnette et al Nat Cell Biol. 2011 Apr;13(4):371-81. do

Insert

Vinculin (VCL Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
qTAG-KO-PGK-Puro

Plasmid

#227264

Purpose

Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mouse Clock delta 19

Plasmid

#177312

Purpose

expression of mutant mouse clock

Depositor

Joseph Takahashi

Article

King et al Cell. 1997 May 16;89(4):641-53. doi: 10.

Insert

mouse clock delta 19

Expression

Mammalian

Mutation

deletion from original mClock gene (514-564aa)

Promoter

CMV

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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REM1

Plasmid

#55681

Purpose

expression of mouse REM1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

REM1 (Rem1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
RP185 pUAST EGFP:Or47b

Plasmid

#59525

Purpose

N-terminal fusion of GFP to Drosophila melanogaster Or47b

Depositor

Leslie Vosshall

Article

Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

Insert

EGFP:Or47b (Or47b Fly)

Tags

EGFP

Expression

Insect

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pSens-4OmeNB

Plasmid

#216231

Purpose

Expresses GFP in response to 4'-O-Methylnorbelladine

Depositor

Simon d'Oelsnitz, Andrew Ellington

Article

d'Oelsnitz et al Nat Commun. 2024 Mar 7;15(1):2084. doi:

Inserts

RamR-4NB2
sfGFP

Use

Synthetic Biology

Mutation

K64T, L67M, C135D, S138G

Promoter

J23103 and Pramr

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pNDGG054

Plasmid

#231323

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid with stability. L1-RK2-Km-par-ELT4; GG assembly from pOGG004, pNDGG001, pOGG010, pOGG012, and pOGG014, NmR/KmR

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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Cas9 Null

Plasmid

#198727

Purpose

Expresses Cas9 without a mobility signal and the mRNA is not graft mobile. Induced by estradiol.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

Cas9

Expression

Plant

Promoter

pG10-90

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-DeltaJunD

Plasmid

#68547

Purpose

AAV expression of DeltaJunD

Depositor

Eric Nestler

Article

Winstanley et al J Neurosci. 2007 Sep 26;27(39):10497-507

Insert

DeltaJunD-IRES-GFP

Use

AAV

Expression

Mammalian

Mutation

truncated splice variant

Promoter

CMV

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only
tph-1(short)::rpl-22-HA

Plasmid

#122203

Purpose

C. elegans tagged ribosome expression plasmid

Depositor

Steven Flavell

Article

Rhoades et al Cell. 2019 Jan 10;176(1-2):85-97.e14. do

Insert

tph-1(short)::rpl-22-HA

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pQTEV-MPP6

Plasmid

#34806

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

membrane protein, palmitoylated 6 (PALS2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pNubWtXgate

Plasmid

#105079

Purpose

Yeast mating-based Split Ubiquitin Assay, positive control

Depositor

Wolf Frommer

Article

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

NubI

Expression

Yeast

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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