We narrowed to 66,338 results for: nin

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pCRISPR-dCas9

Plasmid

#125687

Purpose

Gene knockdown for actinomycetes

Depositor

Tilmann Weber

Publication

Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

Insert

streptomyces codon optimized spdCas9, sgRNA casette

Use

CRISPR and Synthetic Biology

Promoter

ermE*/tipA

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBSDONR P1-P4

Plasmid

#71744

Purpose

ampicillin resistant derivative of pDONR221 P1-P4 for multisite GATEWAY cloning

Depositor

Roger Innes

Publication

Gu et al Plant Physiol. 2011 Apr;155(4):1827-38.

Type

Empty backbone

Use

Gateway entry vector for use in multisite gateway…

Available since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only

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gRNA_DNMT3a-T2

Plasmid

#41822

Purpose

Expresses a guide RNA (gRNA) to target DNMT3a (T2 target sequence) for genome engineering

Depositor

George Church

Publication

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_DNMT3a-T2 (DNMT3A Human)

Use

CRISPR

Expression

Mammalian

Available since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-N 16E5

Plasmid

#37874

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

HPV 16 E5 (E5 )

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

July 25, 2012

Availability

Academic Institutions and Nonprofits only
pCR-blunt-TOPO-FNDC5

Plasmid

#35970

Depositor

Bruce Spiegelman

Publication

Bostrom et al Nature. 2012 Jan 11;481(7382):463-8. doi

Insert

fibronectin type III domain containing 5 (Fndc5 Mouse)

Use

Cloning vector

Available since

April 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEM-human TCR zeta/2470

Plasmid

#11507

Depositor

Allan Weissman

Publication

Weissman et al Proc Natl Acad Sci U S A. 1988 Dec . 85(

Insert

TCR zeta (CD247 Human)

Use

General cloning

Mutation

n/a

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pET28CLY9

Plasmid

#21769

Depositor

Maarten Merkx

Publication

Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

Insert

ECFP -EYFP linked by flexible linker containing 9 SGGSGG repeats

Tags

His

Expression

Bacterial

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
His6TEV-UbG76C

Plasmid

#75299

Purpose

Bacteria expression plasmid for Ubiquitin G76C with removable His6 tag by TEV protease

Depositor

Cynthia Wolberger

Publication

Morgan et al Science. 2016 Feb 12;351(6274):725-8. do

Insert

human Ubiquitin mutant G76 to C

Tags

His

Expression

Bacterial

Mutation

changed Glycine 76 to Cystine

Promoter

T7

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAAV-Syn-SomaGCaMP6f2

Plasmid

#158756

Purpose

Cell body-targeted GCaMP6f, under synapsin promoter: GCaMP6f followed by a linker, and the EE-RR coiled-coil motif (GCaMP6f-27-EE-RR). As bright as conventional GCaMP6f.

Depositor

Edward Boyden

Publication

Shemesh et al Neuron. 2020 Jun 26. pii: S0896-6273(20)

Insert

SomaGCaMP6f2

Use

AAV

Expression

Mammalian

Promoter

synapsin promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pGTag-TagRFP-SV40

Plasmid

#117807

Purpose

Donor for precise CRISPR directed genomic integration using the GeneWeld method

Depositor

Jeffrey Essner

Publication

Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

Insert

TagRFP

Use

Synthetic Biology

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pB-CAGGS-dCas9

Plasmid

#110823

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, George Church

Publication

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBabe_puro_DEST_Flag_PLD1

Plasmid

#45268

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PLD1 (PLD1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLD-puro-2A-rtTA-TcVA

Plasmid

#24592

Depositor

Jason Moffat

Publication

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-EML4-ALK variant 3a

Plasmid

#183829

Purpose

Express EML4-ALK fusion variant 3a (E6a;A20) in mammalian cells

Depositor

Aaron Hata

Publication

Yoda et al Cancer Discov. 2018 Jun;8(6):714-729. do

Inserts

ALK Receptor Tyrosine Kinase (ALK Human)
EMAP Like 4 (EML4 Human)

Use

Lentiviral

Tags

The fusion protein of EML4 exon 1-6a and ALK exon…

Expression

Mammalian

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC 1-331

Plasmid

#16508

Depositor

Bert Vogelstein

Publication

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Expression

Mammalian

Mutation

Codon 331 truncation

Available since

April 30, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
Active Wnt7A-V5

Plasmid

#43816

Depositor

Xi He

Publication

MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

Insert

human WNT7A (WNT7A Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMU-TPr

Plasmid

#61189

Purpose

Fluorescent reporter for tonoplast AtTIP1-1-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Publication

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

AtTIP1-1

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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AIMTOR A757

Plasmid

#140829

Purpose

AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

Depositor

Anne Bonnieu

Publication

Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

Insert

AIMTOR A757 (ULK1 Human)

Tags

NES Nuclear export signal

Expression

Mammalian

Mutation

The insert comprises only a small sequence of hum…

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-LiLac

Plasmid

#184570

Purpose

Fluorescent reporter for lactate (with lifetime and intensity response); expresses in mammalian cells and can be packaged as AAV

Depositor

Gary Yellen

Publication

Koveal et al Nat Commun. 2022 May 25;13(1):2919. doi:

Has service

AAV8

Insert

LiLac fluorescent sensor of lactate

Use

AAV

Tags

His7

Expression

Mammalian

Promoter

CAG

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET30a-HS-Tn5

Plasmid

#127916

Purpose

Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

Depositor

Silin Zhong

Publication

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Hyperstable Tn5

Tags

E coli elongation factor Ts and Mxe intein - Chit…

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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