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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 62,264 results for: SAP

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Showing: 41 - 80 of 62264 results

  3. pOpen-SapIR

    Plasmid
    #165537
    Purpose
    Type II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    SapI
    Use
    Synthetic Biology
    Available Since
    Aug. 5, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165537-plasmid-map-sequence-id-339127

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  16. pSaPIPLC His

    Plasmid
    #173810
    Purpose
    Expression and purification of mature, His-tagged Staphylococcus aureus (strain FPR3757) phosphaatidyl inositol specific phospholipase C (PI-PLC) from E. coli BL21 CodonPlus (DE3) RIL cells
    Depositor
    Anne Gershenson, Mary F Roberts
    Article
    Goldstein et al Biochemistry. 2012 Mar 27;51(12):2579-87
    Insert
    Staphylococcus aureus plc
    Tags
    6XHis
    Expression
    Bacterial
    Mutation
    none
    Promoter
    T7
    Available Since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173810-plasmid-map-sequence-id-341162

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  20. mT-Sapphire-CytERM-N-17

    Plasmid
    #56278
    Purpose
    Localization: Endoplasmic Reticulum, Excitation: 399, Emission: 511
    Depositor
    Michael Davidson
    Insert
    CytERM
    Tags
    mT
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56278-plasmid-map-sequence-id-183147

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  21. mT-Sapphire-H2B-6

    Plasmid
    #56404
    Purpose
    Localization: Nucleus/Histones, Excitation: 399, Emission: 511
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    mT
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. pRiboI SapNde

    Plasmid
    #54490
    Purpose
    Theophylline-inducible expression in single-copy integrating vector
    Depositor
    Jessica Seeliger
    Type
    Empty backbone
    Promoter
    hsp60
    Available Since
    Aug. 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    54490-plasmid-map-sequence-id-86110

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  34. p426_Cas9_gRNA-SAP155c

    Plasmid
    #87390
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting SAP155c sequence ATGAAAGACAACTATAGGGC in yeast chromosome 6
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting SAP155c
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87390-plasmid-map-sequence-id-193432

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  35. p426_Cas9_gRNA-SAP155b

    Plasmid
    #87389
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting SAP155b sequence GGTTTTCATACTGGGGCCGC in yeast chromosome 6
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting SAP155b
    Use
    CRISPR
    Promoter
    ADH1, pTyrosine
    Available Since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87389-plasmid-map-sequence-id-193431

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  38. pC178: pAAV.U6.SapI.CMV.Cas13bt3

    Plasmid
    #204215
    Purpose
    Plasmid AAV vector expressing Cas13bt3 and hU6-driven expression of guide RNAs. Contains SapI sites for guide cloning flanked by 5' and 3' full-length DRs.
    Depositor
    Guei-Sheung Liu
    Article
    Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121
    Insert
    Cas13bt3 and U6.SapI sgRNA cloning site
    Use
    AAV and CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Mutation
    Codon optimisation by GenScript tool
    Promoter
    CMV/U6
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    204215-plasmid-map-sequence-id-455913

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Showing: 41 - 80 of 62264 results