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Plasmid#227269PurposeEmpty AAVS1 targeting donor for the insertion of Puro and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only
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qTAG-AAVS1-PGK-Zeo
Plasmid#227271PurposeEmpty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-mPGK-eGFP (beta-globin intron)
Plasmid#162513PurposeAAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the mouse phosphoglycerate kinase (mPGK) promoter that is upstream of a beta-globin intronDepositorInserteGFP
UseAAVTagsExpressionMammalianMutationPromotermPGKAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCLJ-P2F-Frt-PGK-Puro-Frt
Plasmid#227501PurposeInserts 500bp Fgf5 promoter in place of the Prdm8 promoter along with removable selection cassetteDepositorInsertFgf5 promoter
UseAAV and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCLJ-F2P-LoxP-PGK-PuroR-LoxP
Plasmid#227502PurposeInserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassetteDepositorInsertPrdm8 promoter
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCLJ-F2P-LoxP-PGK-NeoR-LoxP
Plasmid#227503PurposeInserts 500bp Prdm8 promoter in place of the Fgf5 promoter along with removable selection cassetteDepositorInsertPrdm8 promoter
UseAAV and Cre/LoxTagsExpressionMutationPromoterAvailable sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
MSCV_hU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-Thy1.1
Plasmid#187925PurposesgRNA vector backbone expressing puromycin resistance and CD90.1 as selection markersDepositorInserthU6_BbsI-ccdB-BbsI_iScaffold_mPGK_puro-2A-CD90.1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK
Plasmid#79120PurposeLentiviral vector for constitutive expression of genes (PGK promoter)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
MXS_PGK
Plasmid#62414PurposeMXS_chaining vector with PGKDepositorInsertPGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGKO1
Plasmid#63611Purposea binary Agrobacterium vector carrying the HSVtk gene as a negative selection marker against ectopic transformants.DepositorTypeEmpty backboneUseBinary fungal vectorTagsExpressionMutationPromoterLac PromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
MXS_minPGK
Plasmid#62415PurposeMXS_chaining vector with minPGKDepositorInsertMinimal PGK Promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
C453-E17: attB4-PGKp>-attB5
Plasmid#162976PurposeGateway attB4/attB5 entry clone for mammalian expression using the human PGK promoterDepositorInserthuman pGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
C413-E21: attB4-PGKp>-attB1r
Plasmid#162921PurposeGateway attB4/attB1r entry clone for mammalian expression using the human PGK promoterDepositorInserthuman pGK promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP
Plasmid#117140PurposeExpress gRNA vs Dmap1DepositorInsertgDmap1 v1 (Dmap1 Synthetic)
UseCRISPR and LentiviralTagsExpressionMutationDeleted BbsI site within WPRE, modified BFPPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)
Plasmid#172330PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianMutationPromoterPGK/CMVAvailable sinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-NRAS(G12V)
Plasmid#35632DepositorInsertN-Ras(G12V) (NRAS Human)
UseLentiviralTagsHAExpressionMammalianMutationGlycine 12 to ValinePromoterPGKAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCWX-R4DESTR2-PGK-BSD
Plasmid#114320PurposeLentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Blasticidin resistanceDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWX-R4DESTR2-PGK-PURO
Plasmid#114319PurposeLentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Puromycin resistanceDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
U6-PGK-hCD2
Plasmid#224294PurposeExpresses sgRNA from U6 promoter and human CD2 under PGKDepositorInsertU6 sgRNA cassette with CD2 under PGK promoter (CD2 Human)
UseCRISPR and RetroviralTagsExpressionMammalianMutationPromoterU6/PGKAvailable sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pucKan_ARS_pgkZeo_ncx1_mCherry_2TA_Puro_ARS
Plasmid#163755Purposepiggybac transposon encoding human Ncx1 gene promoter-driven mCherry and puromycin-acetyltransferase for labelling and selection of pluripotent stem cell-derived cardiomyocytesDepositorInsertmCherry
UsePiccybac transposonTagspuromycin-N-acetyltransferaseExpressionMammalianMutationPromoterhuman NCX1Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG- gate-pGK-HYG
Plasmid#107397PurposeGateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCWX-R4DESTR2-PGK-Tk:Sh
Plasmid#114321PurposeLentiviral backbone for Gateway cloning of Promoter/Gene insert together with constitutive Zeocin/Bleomycin resistance and Acyclovir conditional ablationDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
plenti-UBC-HA-gate-pGK-HYG
Plasmid#107396PurposeLentiviral gateway destination vectorDepositorTypeEmpty backboneUseLentiviral; Gateway destinationTagsHAExpressionMammalianMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-KRAS4B(G12V)
Plasmid#35633DepositorInsertK-Ras4B(G12V) (KRAS Human)
UseLentiviralTagsHAExpressionMammalianMutationGlycine 12 to ValinePromoterPGKAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-ER-KRAS(G12V)
Plasmid#35635DepositorInsertK-Ras4B(G12V) (KRAS Human)
UseLentiviralTagsERExpressionMammalianMutationGlycine 12 to ValinePromoterPGKAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-KRAS4A(G12V)
Plasmid#35634DepositorInsertK-Ras4A(G12V) (KRAS Human)
UseLentiviralTagsV5ExpressionMammalianMutationGlycine 12 to ValinePromoterPGKAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_synNotch_Gal4VP64
Plasmid#79125PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_Gal4VP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_antiCD19_synNotch_TetRVP64
Plasmid#79126PurposeLentiviral vector for constitutive expression of the anti-CD19 scFv synNotch TetRVP64 Receptor (PGK promoter)DepositorInsertantiCD19_synNotch_TetRVP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_pGK_LaG17_synNotch_Gal4VP64
Plasmid#79127PurposeLentiviral vector for constitutive expression of the LaG17 GFP nanobody synNotch Gal4DBDVP64 Receptor (PGK promoter)DepositorInsertLaG17_synNotch_Gal4DBDVP64
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)
Plasmid#203908PurposeEncodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cellsDepositorInsertGal4UAS_dGFP_PGK_iRFP
UseTagsExpressionMammalianMutationPromoter5xUAS and PGKAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti.PGK.H2B-chFP.W
Plasmid#51007PurposeLentivector that expresses H2B-chFP from internal human PGK promoterDepositorInsertH2B-ChFP
UseLentiviralTagshistone2BExpressionMammalianMutationPromoterhuman PGKAvailable sinceJune 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT2/CMV-Luc/PGK-SB100X
Plasmid#192869PurposeCarries luciferase under CMV promoter flanked with IR/DR elements. Also carries SB100X under PGK promoter outside of the IR/DR elements.DepositorInsertsluc
SB100X
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-KRAB-2A-Puro
Plasmid#129536PurposePiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.DepositorInsertPB-PGK-tetR-KRAB-2A-Puro
UseUnspecifiedTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGK-HELLO-T2A-Cre LV
Plasmid#202003PurposeGenerates lentiviral vector for autochthonous KP-HELLO tumor initiation by intratracheal administration to KP miceDepositorInsertHELLO-T2A-Cre
UseLentiviralTagsFLAGExpressionMutationPromoterpGKAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-TRE3G-PLOD2_PGK-GpNLuc
Plasmid#136457PurposeExpresses Tet/Dox-inducible human PLOD2 in mammalian cells.DepositorInsertTRE3G::PLOD2-T2A-Hygromycin (PLOD2 Human)
UseLuciferase and RetroviralTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGKCMV)-nucTomato-hygro
Plasmid#208573PurposeLV REPORT containing minimal promoter driving monomeric nuclear Tomato followed by an PGK/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
d2nucEGFP
HygroR
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT(PGK/CMV)-GtwyA
Plasmid#208574PurposeLV REPORT containing minimal promoter (GtwyA inserted into MCS) driving monomeric nuclear Tomato followed by an PKG/CMV promoter driving nuclear d2eGFP E2A HygromycinDepositorInsertsmnucTomato
HSVtk
Neomycin
d2nucEGFP
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterPGK/CMV and minPAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro
Plasmid#129537PurposePiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.DepositorInserttetR-3xFlag-HA-2A-Puro
UseSynthetic BiologyTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSII-PGK-FLPo-IRES-Puro
Plasmid#205989PurposeExpresses Flp recombinase and puromycin resistanceDepositorInsertsUseTagsExpressionMammalianMutationPromoterPGK promoterAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only