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We narrowed to 100 results for: Toxoplasma gondii

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Showing: 21 - 40 of 100 results

  1. pCvGAPDH-CvH2B-mStayGold

    Plasmid
    #255783
    Purpose
    Expresses mStayGold-tagged Chromera histone 2b protein (VEupathDB: Cvel_19200) in Chromera velia and Toxoplasma gondii
    Depositor
    Ke Hu
    Publication
    Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400
    Insert
    Chromera velia histone H2B (VEupathDB: Cvel_19200)
    Use
    Chromera velia, toxoplasma gondii
    Tags
    mStayGold (codon-optimized for Chromera)
    Expression
    Bacterial
    Promoter
    putative C. velia GAPDH promoter (1090 bp)
    Available since
    June 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    255783-plasmid-map-sequence-id-511279

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  2. pCvGAPDH-CvACP-mStayGold

    Plasmid
    #255784
    Purpose
    Expresses mStayGold-tagged Chromera acyl carrier protein (VEupathDB: Cvel_32060) in Chromera velia and Toxoplasma gondii
    Depositor
    Ke Hu
    Publication
    Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400
    Insert
    Chromera velia acyl carrier protein (VEupathDB: Cvel_32060)
    Use
    Chromera velia, toxoplasma gondii
    Tags
    PGTGSGGTGSGRS linker and mStayGold (codon-optimiz…
    Expression
    Bacterial
    Promoter
    putative C. velia GAPDH promoter (1090 bp)
    Available since
    June 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    255784-plasmid-map-sequence-id-511277

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  3. pCvGAPDH-CvIMC13-mStayGold

    Plasmid
    #255785
    Purpose
    Expresses mStayGold-tagged Chromera IMC13 protein (VEupathDB: Cvel_9514) in Chromera velia and Toxoplasma gondii
    Depositor
    Ke Hu
    Publication
    Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400
    Insert
    Chromera velia inner membrane complex 13 protein (VEupathDB: Cvel_9514)
    Use
    Chromera velia, toxoplasma gondii
    Tags
    PGTGSGGTGSGRS linker and mStayGold (codon-optimiz…
    Expression
    Bacterial
    Promoter
    putative C. velia GAPDH promoter (1090 bp)
    Available since
    June 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    255785-plasmid-map-sequence-id-511278

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  4. pU6-Universal.Sth.dCas9

    Plasmid
    #171088
    Purpose
    CRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Toxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
    DHFR-TSc3
    Use
    Toxoplasma gondii expression
    Mutation
    Note: A S245F mutation is present but did not app…
    Promoter
    DHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171088-plasmid-map-sequence-id-335785

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  6. pCvGAPDH-mStayGold-CvTubA1

    Plasmid
    #255786
    Purpose
    Expresses mStayGold-tagged Chromera tubulin alpha chain 1 (VEupathDB: Cvel_22324) in Chromera velia and Toxoplasma gondii
    Depositor
    Ke Hu
    Publication
    Tengganu et al J Cell Sci. 2026 Jan 1;139(1):jcs.264400
    Insert
    Chromera velia tubulin subunit alpha 1 (VEupathDB: Cvel_22324)
    Use
    Chromera velia, toxoplasma gondii
    Tags
    PGTGSGGTGSGRS linker and mStayGold (codon-optimiz…
    Expression
    Bacterial
    Promoter
    putative C. velia GAPDH promoter (1090 bp)
    Available since
    June 10, 2026
    Availability
    Academic Institutions and Nonprofits only
    255786-plasmid-map-sequence-id-511275

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  10. pTUB1:YFP-AID-3HA, DHFR-TS:HXGPRT

    Plasmid
    #87260
    Purpose
    YFP-AID-3HA fusion driven by a minimal TUB1 promoter with an HXGPRT drug selectable marker. The AID CDS is Arabidopsis thaliana auxin-responsive protein IAA17.
    Depositor
    David Sibley
    Publication
    Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d
    Inserts
    YFP-AID-3HA
    HXGPRT
    Use
    Toxoplasma expression
    Tags
    3HA and AID
    Promoter
    TgDHFR-TS 463 bp and TgTUB1 378 bp
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    87260-plasmid-map-sequence-id-176554

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  11. pTet-On-TAP

    Plasmid
    #59017
    Purpose
    Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.
    Depositor
    David Sibley
    Publication
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Type
    Empty backbone
    Use
    Toxoplasma expression
    Tags
    Calmodulin Binding Peptide fused with a TEV Cleav…
    Promoter
    RPS13 with Tet Operators Inserted
    Available since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59017-plasmid-map-sequence-id-423220

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  13. ptdTomato.LIC.HXGPRT

    Plasmid
    #83365
    Purpose
    Plasmid contains LIC cassette upstream of an in-frame copy of tdTomato and HXGPRT selectable marker cassette for endogenous tagging on the gene locus
    Depositor
    Vernon Carruthers
    Publication
    Huynh et al Eukaryot Cell. 2009 Apr;8(4):530-9. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tdTomato
    Use
    Toxoplasma gondii
    Tags
    tdTomato
    Available since
    Nov. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    83365-plasmid-map-sequence-id-165801

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  17. pCFP.HXGPRT

    Plasmid
    #83366
    Purpose
    Plasmid contains LIC cassette upstream of an in-frame copy of CFP and HXGPRT selectable marker cassette for endogenous tagging on the gene locus
    Depositor
    Vernon Carruthers
    Publication
    Huynh et al Eukaryot Cell. 2009 Apr;8(4):530-9. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CFP
    Use
    Toxoplasma gondii
    Tags
    CFP
    Available since
    Nov. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    83366-plasmid-map-sequence-id-165803

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  18. MIC2

    Plasmid
    #50050
    Purpose
    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2
    Tags
    HIS, SBP, and TEV
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CMV
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  19. MIC2

    Plasmid
    #50050
    Purpose
    expresses toxoplasma gondii MIC2 extracellular domain in mammalian cells
    Depositor
    Timothy Springer
    Insert
    MIC2
    Tags
    HIS, SBP, and TEV
    Expression
    Mammalian
    Mutation
    S158A, N357S, N463S, N470D
    Promoter
    CMV
    Available since
    April 14, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 21 - 40 of 100 results