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Plasmid#65338Purposeconstruction of CYC1 promoter in HCKan-P receiving vectorDepositorInsertCYC1 promoter
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pD221 Cyc1_term
Plasmid#58562PurposepDONR221 carrying the Cyc1 terminatorDepositorInsertCyc1 terminator
UseGateway entryTagsExpressionMutationPromoterAvailable sinceSept. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTO135_pENO1-CaCas9-tCyc1
Plasmid#171103PurposeExpresses Cas9 in Candida aurisDepositorInsertCaCas9
UseTagsExpressionYeastMutationPromoterENO1Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCYC1pr-GNSI (LEU)
Plasmid#111450PurposeGNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
UseTagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …PromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315-SEC22-NCYC110
Plasmid#226273PurposePlasmid expressing the SEC22 allele from NCYC110, under control of its native promoterDepositorInsertSEC22 (SEC22 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SEC18-NCYC110
Plasmid#226277PurposePlasmid expressing the SEC18 allele from NCYC110, under control of its native promoterDepositorInsertSEC18 (SEC18 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SSD1-NCYC110
Plasmid#226279PurposePlasmid expressing the SSD1 allele from NCYC110, under control of its native promoterDepositorInsertSSD1 (SSD1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS313-SCT1-NCYC110
Plasmid#226269PurposePlasmid expressing the SCT1 allele from NCYC110, under control of its native promoterDepositorInsertSCT1 (SCT1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRS426-PGAL7-A_GFP_TCYC1
Plasmid#177162PurposeExpresses yeGFP under the control of a synthetic promoter (PGAL7-A)DepositorInsertyeGFP
UseTagsExpressionYeastMutationPromoterSynthetic promoter (PGAL7-A)Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-PGAL7-B_GFP_TCYC1
Plasmid#177163PurposeExpresses yeGFP under the control of a synthetic promoter (PGAL7-B)DepositorInsertyeGFP
UseTagsExpressionYeastMutationPromoterSynthetic promoter (PGAL7-B)Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-PGAL7-C_RFP_TCYC1
Plasmid#177164PurposeExpresses yeRFP under the control of a synthetic promoter (PGAL7-C)DepositorInsertyeCherry
UseTagsExpressionYeastMutationPromoterSynthetic promoter (PGAL7-C)Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRS426-PGAL7-D_RFP_TCYC1
Plasmid#177165PurposeExpresses yeRFP under the control of a synthetic promoter (PGAL7-D)DepositorInsertyeCherry
UseTagsExpressionYeastMutationPromoterSynthetic promoter (PGAL7-D)Available sinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
LHP809 - Ub-K63A-CYC1
Plasmid#32166DepositorUseTagsExpressionYeastMutationK63APromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
p414-TEF1p-Cas9-CYC1t
Plasmid#43802PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterTEF1 promoterAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterGalLAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
PBAD-his6-prkA-pACYC184
Plasmid#41041DepositorInsertprkA
UseTags6xHis and T7 epitope tagExpressionBacterialMutationPromoterPBADAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#83946PurposeCas9-carrying vector with kanMX markerDepositorInsertCas9
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1
Plasmid#41023DepositorInserttetO7-Pcyc1
UsePromoter replacementTagsExpressionMutationPromoterAvailable sinceDec. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pTEF1p-CYC1t
Plasmid#62190PurposeExpresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionYeastMutationhuman codon-optimized, D10A, H840APromoterTEF1 promoterAvailable sinceFeb. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC002 - LshC2C2 locus into pACYC184
Plasmid#79151PurposeEndogenous LshC2c2 locus cloned into pACYC184DepositorInsertLshC2c2 locus
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only