Skip to main content
Addgene
Showing: 21 - 40 of 2752 results

  1. pGGF_RT_FAST_Tq2CFP

    Plasmid
    #136981
    Purpose
    Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence
    Depositor
    Klaus Theres
    Insert
    seed coat marker, replaced RFP w/ Tq2CFP. Replaced NOS terminator w/ Arabidopsis HSP18-2 terminator
    Use
    Tags
    Expression
    Bacterial
    Mutation
    BsaI site domestication in pOLE1:OLE1-RFP 688 G &…
    Promoter
    Available since
    Jan. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    136981-plasmid-map-sequence-id-269096

    Enlarge

  2. FASN

    Plasmid
    #38793
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FASN (FASN Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. FASN

    Plasmid
    #38794
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    FASN (FASN Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. pFastBacHTa_LYN

    Plasmid
    #224244
    Purpose
    Expression of human LYN Kinase in insect cells (SF9 cells)
    Depositor
    Andrew Mesecar
    Insert
    lyn (LYN Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224244-plasmid-map-sequence-id-458379

    Enlarge

  8. pFastBacHTa_SYK

    Plasmid
    #224246
    Purpose
    Expression of human SYK Kinase in insect cells (SF9 cells)
    Depositor
    Andrew Mesecar
    Insert
    syk (SYK Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    224246-plasmid-map-sequence-id-458376

    Enlarge

  9. ER-FAST

    Plasmid
    #216340
    Purpose
    For testing localization of pFAST protein to endoplasmic reticulum
    Depositor
    Alison Tebo
    Insert
    PRL_pFAST
    Use
    Tags
    myc for FAST protein
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    CMV
    Available since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216340-plasmid-map-sequence-id-427398

    Enlarge

  11. PA-mCherry1-Fascin-C-10

    Plasmid
    #57177
    Purpose
    Localization: Cytoskeleton, Excitation: 400 / 504, Emission: 515 / 517
    Depositor
    Michael Davidson
    Insert
    Fascin (FSCN1 Human)
    Use
    Tags
    PA-mCherry1
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    57177-plasmid-map-sequence-id-249188

    Enlarge

  12. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

    Enlarge

  14. SPLICSFAST-ER-PO-P2A

    Plasmid
    #214894
    Purpose
    Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-ER-PO-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214894-plasmid-map-sequence-id-423480

    Enlarge

  17. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

    Enlarge

Showing: 21 - 40 of 2752 results