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Showing: 571 - 600 of 624 results

  2. qTAG-N-Solo-mStayGold-GOLGA2

    Plasmid
    #227325
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the GOLGA2 locus. For Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 (Addgene #207791)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a mStayGold Tag (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Promoterless
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227325-plasmid-map-sequence-id-452110

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  4. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid
    #140163
    Purpose
    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker
    Depositor
    Benjamin Glick
    Article
    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.
    Insert
    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Human, Synthetic)
    Use
    Tags
    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140163-plasmid-map-sequence-id-275937

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  5. qTAG-N-Puro-moxGFP-GOLGA2

    Plasmid
    #207792
    Purpose
    Donor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    GOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207792-plasmid-map-sequence-id-413795

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  6. pLS2965

    Plasmid
    #122534
    Purpose
    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_aman-2::CemOrange2 (aman-2 Nematode, Synthetic)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122534-plasmid-map-sequence-id-238120

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  13. pFP5299 ss-FIREmate-HA- KDEL-IVS-IRES- ManII-SBP- mApple-FIREtag

    Plasmid
    #216695
    Purpose
    Synchronize anterograde and retrograde trafficking of ManII between ER and Golgi (RUCH system)
    Depositor
    Franck Perez
    Article
    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s
    Inserts
    FIREmate-KDEL
    ManII-SBP-mApple-FIREtag (Man2a1 Mouse)
    Use
    Tags
    FIREtag, Streptavidin Binding Protein (SBP), and …
    Expression
    Mammalian
    Mutation
    contains only amino acids 1 to 116 of ManII
    Promoter
    CMV
    Available since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    216695-plasmid-map-sequence-id-429706

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  16. pcDNA3/td5StayGold(c4)=GianCreg

    Plasmid
    #212022
    Purpose
    For labeling the Golgi apparatus. c4 is the appendage for N-terminal tagging with td5StayGold. ‘GianCreg’ is an abbreviation for giantin C-terminal region. ‘=’ denotes a linker [(GGGGS)3].
    Depositor
    Atsushi Miyawaki
    Article
    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s
    Insert
    GianCreg (GOLGB1 Human)
    Use
    Tags
    td5StayGold(c4)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    212022-plasmid-map-sequence-id-419221

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Showing: 571 - 600 of 624 results