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pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187949

Purpose

SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

SMASh-SpdCas9-tagRFPt-P2A-tagBFP

Use

CRISPR and Synthetic Biology

Tags

GGS linker

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC H-Ras V12

Plasmid

#18741

Depositor

Scott Lowe

Article

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

H-Ras V12 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V12

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
EGFP-Tau(T217A)

Plasmid

#187025

Purpose

Expresses EGFP tagged human 2N4R tau (with T217A mutation) in mammalian cells with CMV promoter

Depositor

George Bloom

Article

Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

Insert

MAPT (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Changed Threonine 217 to Alanine

Promoter

CMV

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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MSCV eIF4E E103A IRES GFP

Plasmid

#18766

Depositor

Scott Lowe

Article

Wendel et al Genes Dev. 2007 Dec 15. 21(24):3232-7.

Insert

eIF4E E103A (Eif4e Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Mutation

E103A

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pDEST_FLAG-HUWE1_BH3-4E

Plasmid

#187144

Purpose

mammalian cell expression of FLAG-HUWE1 V1976E/V1780E/L1983E/M1987E

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

V1976E/V1780E/L1983E/M1987E

Promoter

Available since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_FLAG-HUWE1_delta3291-3298

Plasmid

#187125

Purpose

mammalian cell expression of FLAG-HUWE1 delta3291-3298

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Use

Tags

1xFLAG

Expression

Mammalian

Mutation

delta3291-3298

Promoter

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSBK.013

Plasmid

#187213

Purpose

Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT]), and Cas1 + Cas2

Depositor

Seth Shipman

Article

Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

Inserts

Retron Eco1 ncRNA v35 (long a1/a2, barcode 5 [ACG-CGT])
Cas1 + Cas2

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

T7/Lac

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_DUX4

Plasmid

#187799

Purpose

MAC-tagged gene expression of human DUX4

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

DUX4 (DUX4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_EPHB6

Plasmid

#187763

Purpose

MAC-tagged gene expression of human EPHB6

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

EPHB6 (EPHB6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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RFP-FKBP-U2AF35

Plasmid

#187969

Purpose

expresses U2AF35 with N-terminal tags RFP and FKBP

Depositor

Markus Wahl

Article

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

U2AF35 (U2af1 Mouse)

Use

Cmv promoter

Tags

mRFP-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe H-Ras V12 C40

Plasmid

#18747

Depositor

Scott Lowe

Insert

H-Ras V12 C40 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V12 C40

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
p3xFLAG-Tmprss6(Mask)

Plasmid

#18790

Depositor

Bruce Beutler

Article

Du et al Science. 2008 May 23. 320(5879):1088-92.

Insert

Type II transmembrane serine protease 6 (Tmprss6 Mouse)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 567-811 and replaced with 28 …

Promoter

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
CMV-hCas9-T2A-miRFP670

Plasmid

#187223

Purpose

Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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RFP-FKBP-PRPF38A

Plasmid

#187968

Purpose

expresses PRPF38A with N-terminal tags RFP and FKBP

Depositor

Markus Wahl

Article

Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi:

Insert

Prp38A (PRPF38A Human, Synthetic)

Use

Cmv promoter

Tags

mRFP-FKBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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RGS-SENP5m

Plasmid

#18715

Depositor

Edward Yeh

Article

Gong et al J Biol Chem. 2006 Jun 9. 281(23):15869-7

Insert

SENP5m (SENP5 Human)

Use

Tags

RGS

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
Cherry-Myo1b

Plasmid

#187366

Purpose

Expresses rat myosin 1b isoform b domain labelled with Cherry

Depositor

Evelyne Coudrier

Article

Iuliano et al J Cell Biol. 2018 Jun 4;217(6):2033-2046

Insert

myosin 1b isoform b (Myo1b Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe H-Ras V12 S35

Plasmid

#18746

Depositor

Scott Lowe

Insert

H-Ras V12 S35 (HRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

V12 S35

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
Thy1-Brainbow-1.0 H

Plasmid

#18724

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

tdimer2 ; M-mEYFP ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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Cherry-Plekhh1

Plasmid

#187372

Purpose

Expresses human Plekhh1 labelled with Cherry

Depositor

Evelyne Coudrier

Article

Prosperi et al J Cell Sci. 2021 Dec 1;134(23). pii: 272

Insert

Plekhh1

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_v1_Dual_epegRNA_tevopreQ1

Plasmid

#187458

Purpose

Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 epegRNA (tevopreQ1) with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N epegRNA (tevopreQ1) (ATP1A1 Human)

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

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