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MAC_N_ELF5

Plasmid

#187748

Purpose

MAC-tagged gene expression of human ELF5

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

ELF5 (ELF5 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pFPV25.1_dGFPmut3-AAV

Plasmid

#187377

Purpose

rpsM promoter driving the expression of destabilized GFPmut3 (containing a AAV C-terminal tail)

Depositor

Roi Avraham

Article

Ciolli Mattioli et al Curr Biol. 2023 Nov 20;33(22):4880-4892.

Insert

dGFPmut3_AAV

Tags

AAV

Expression

Bacterial

Mutation

C-terminal AAV tail

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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px552-sg-gria1-HT-SEP

Plasmid

#187653

Purpose

Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

Depositor

Erin O'Shea

Article

Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

Insert

HaloTag-SEP donor

Use

AAV and CRISPR

Tags

N/A

Promoter

N/A

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_N_PAX7

Plasmid

#187750

Purpose

MAC-tagged gene expression of human PAX7

Depositor

Markku Varjosalo

Article

Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

Insert

PAX7 (PAX7 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TagBFP-P2A-Pre-SPHERE-SF-iGluSnFR

Plasmid

#187089

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Pre-SPHERE-SF-iGluSnFR

Tags

TagBFP-P2A

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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HA-EGFP-NEMO-D304N

Plasmid

#187898

Purpose

Mammalian cell expression of D304N mutation in NEMO

Depositor

Erika Holzbaur

Article

Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

Insert

HA-EGFP-NEMO-D304N (Ikbkg Mouse)

Tags

EGFP and HA

Expression

Mammalian

Mutation

D304N

Promoter

CMV

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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PLL5.0-Anillin ShRNA-Cherry-Anillin deltaMyosin binding domain

Plasmid

#187275

Purpose

Lentiviral expression of shRNA targeting Anillin + reconstitution of Anillin delta Myosin binding domain

Depositor

Alpha Yap

Article

Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12.

Insert

Anillin ,hsAnillin, Entrez Gene ANLN (a.k.a. FSGS8, Scraps, scra) (ANLN Human)

Use

Lentiviral

Tags

mCherry

Mutation

deltaMyosin binding domain

Promoter

U6, CMV

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pE-mApple L5L2

Plasmid

#187690

Purpose

C-Term Gateway cloning tag

Depositor

Mary Beckerle

Insert

mApple

Use

Gateway entry vector

Tags

mApple

Mutation

WT

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-mStrawberry

Plasmid

#187224

Purpose

Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

CRISPR

Tags

Inserted T2A-mStrawberry at 3' terminal of M…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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ATP1A1_T804N_hPGK1_EBFP_Donor

Plasmid

#187455

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N EBFP donor (ATP1A1 Human)

Expression

Mammalian

Promoter

hPGK1

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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DL147_plenti_TRE_HT-iPAK4

Plasmid

#187447

Purpose

Tet-On inducible expression of HaloTag-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box Pak4

Use

Lentiviral

Tags

halo tag

Expression

Mammalian

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

Plasmid

#187945

Purpose

dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

Depositor

Edda Schulz

Article

Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

Insert

SpdCas9-tagRFPt-P2A-tagBFP

Use

Synthetic Biology; Piggybac

Tags

NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt

Expression

Mammalian

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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MAC_C_IGF1R

Plasmid

#187770

Purpose

MAC-tagged gene expression of human IGF1R

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

IGF1R (IGF1R Human)

Expression

Mammalian

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pTripz Casp1-IRES-mCherry

Plasmid

#187294

Purpose

This is Caspase1 from mouse (NM_009807.2) with its CARD domain replaced by the FKBP-V domain (dimerising domain).

Depositor

Alpha Yap

Article

Bonfim-Melo et al Cell Rep. 2022 Feb 1;38(5):110316. doi:

Insert

Caspase1 (Casp1 Mouse)

Use

Lentiviral

Tags

mCherry

Promoter

CMV

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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MAC_C_LMTK2

Plasmid

#187752

Purpose

MAC-tagged gene expression of human LMTK2

Depositor

Markku Varjosalo

Article

Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

Insert

LMTK2 (LMTK2 Human)

Expression

Mammalian

Available Since

Aug. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-CDK6-mutPAM-PGK-puro

Plasmid

#187638

Purpose

sg resistant CDK6 ORF

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

CDK6 (CDK6 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

PAM site mutation

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Thy1-Brainbow-1.0 H

Plasmid

#18724

Depositor

Joshua Sanes

Article

Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

Insert

tdimer2 ; M-mEYFP ; M-mCerulean

Use

Cre/Lox and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pLL5.0 hCortKD-Flcortmut3YD EGFP

Plasmid

#187264

Purpose

Lentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YD mutant

Depositor

Alpha Yap

Article

Liang et al Nat Commun. 2017 Oct 5;8(1):790. doi: 10

Insert

Cortactin (CTTN Human)

Use

Lentiviral

Tags

EGFP

Mutation

3YD (Y412, Y470, Y486)

Promoter

U6, 5'LTR

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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UAS-dsarm(E)

Plasmid

#187870

Purpose

Gal4/UAS expression of dSarm(isoE)

Depositor

Lukas Neukomm

Article

Llobet Rosell et al Elife. 2022 Dec 7;11:e80245. doi: 10.755

Insert

dSarm isoform E (Sarm Fly)

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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GFAP-Archon-EGFP

Plasmid

#187979

Purpose

Expresses the fluorescent voltage indicator Archon under the GFAP promoter

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Archon1-EGFP

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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