We narrowed to 24,716 results for: Nov

1. pAS1_4xh7SKPylT_EF1_BsmBI_(C1A)-Intein_DD

   Plasmid

   #162811

   Purpose

   Control STELLA destination plasmid for C-terminal labelled proteins. Contains restriction sites for golden gate cloning.

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   BsmBI_(C1A)-Intein_DD
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   Intein(Cys1,Ala)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

   Plasmid

   #162814

   Purpose

   STELLA plasmid for amber suppression of C-terminally labelled SMIM38

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   SMIM38(C-TAG)-Intein-DD
   

   Use

   Piggybac

   Expression

   Mammalian

   Mutation

   SMIM38(Ochre52,Amber)

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS1_4x7SKPylT_EF1_Ub-BsmBI-HA_IRES_BSD

   Plasmid

   #162805

   Purpose

   STELLA destination plasmid with HA terminal tag; Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

   Insert

   Ub-BsmBI-HA_IRES_Blasticidin
   

   Use

   Piggybac

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Membrane protein gRNA library

   Pooled Library

   #113345

   Purpose

   Activation library targeting the promoters of 6,213 genes that encode at least one putative membrane-associated protein isoform.

   Depositor

   Gavin Wright

   Article

   Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

   Species

   Homo sapiens

   Use

   Lentiviral

   Available Since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

5. Yeast Inducible CRISPRi Library

   Pooled Library

   #161829

   Purpose

   Inducible genome-wide CRISPRi library targeting S. cerevisiae genes with 6-12 guide RNAs per gene.

   Depositor

   Saeed Tavazoie

   Article

   Momen-Roknabadi et al Commun Biol. 2020 Nov 27;3(1):723. doi:

   Expression

   Yeast

   Species

   Saccharomyces cerevisiae

   Use

   CRISPR

   Available Since

   April 28, 2021

   Availability

   Academic Institutions and Nonprofits only

6. pGS96_EGFP(A206K)-I3-01 AAVS donor

   Plasmid

   #211865

   Purpose

   I3-01 transgene integration at AAVS1 safe harbor locus

   Depositor

   Iain Cheeseman

   Insert

   I3-01
   

   Use

   CRISPR

   Mutation

   N/A

   Available Since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV_JAK3_IRES_BFP

   Plasmid

   #206291

   Purpose

   Expresses the wild type form of human JAK3.

   Depositor

   Charles de Bock

   Article

   Omari et al Heliyon. 2023 Nov 8;9(11):e22085. doi: 1

   Insert

   JAK3 (JAK3 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCSIIhyg-rtTA

   Plasmid

   #139480

   Purpose

   Expresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   rtTA, Tet-ON reverse transactivator
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Human EF1a promoter

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. nls-EGFP

   Plasmid

   #67652

   Purpose

   Mammalian expression vector driving nls-EGFP.

   Depositor

   Rob Parton

   Article

   Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

   Insert

   nls-EGFP
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB80-2xFKBP-L-mCherry-CAAX

    Plasmid

    #193713

    Purpose

    Rapalog-inducible coupling to plasma membrane (CAAX) via FKBP

    Depositor

    Lukas Kapitein

    Article

    Burute et al Sci Adv. 2022 Nov 4;8(44):eabo2343. doi:

    Insert

    2xFKBP-L-mCherry-CAAX
    

    Tags

    2xFKBP-mCherry

    Expression

    Mammalian

    Mutation

    mCherry: Met1 Deleted

    Promoter

    Chicken beta-actin

    Available Since

    March 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF5B-FRT-cilia-APEX

    Plasmid

    #73186

    Purpose

    Expresses cilia-APEX in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    cilia-APEX (Nphp3 Glycine max, Mouse)
    

    Tags

    internal GFP tag

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-mCherry-LKB1(WT)

    Plasmid

    #84639

    Purpose

    mCherry-tagged, wild-type LKB1

    Depositor

    Jin Zhang

    Article

    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

    Insert

    mCherry-LBK1 (STK11 Synthetic, Human)
    

    Tags

    6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac-CHD7

    Plasmid

    #170141

    Purpose

    Expresses CHD7 in insect cells

    Depositor

    Robert Kingston

    Article

    Bouazoune et al Proc Natl Acad Sci U S A. 2012 Nov 20;10

    Insert

    chromodomain helicase DNA binding protein 7 (CHD7 Human)
    

    Tags

    6xHis and FLAG

    Expression

    Insect

    Available Since

    July 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459-HypaCas9-mR2-AAVS1_sgRNA

    Plasmid

    #183890

    Purpose

    pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX459-HypaCas9-AAVS1_sgRNA

    Plasmid

    #183891

    Purpose

    pX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    AAVS1 sgRNA spacer (AAVS1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. EpsilonCOP EGFP

    Plasmid

    #128151

    Purpose

    Expression of epsilon COP

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

    Insert

    epsilon COP (COPE Bovine)
    

    Expression

    Mammalian

    Available Since

    July 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJW1512

    Plasmid

    #62366

    Purpose

    N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    pTRE2

    Available Since

    April 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX459V2.0-HypaCas9-mRuby2

    Plasmid

    #183872

    Purpose

    pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    HypaCas9-P2A-mRuby2-T2A-Puro

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUASp-GBP-Tbro

    Plasmid

    #179995

    Purpose

    Expresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASp version is ideal for expression in the germline.

    Depositor

    Graydon Gonsalvez

    Article

    Baker et al Development. 2021 Nov 15;148(22). pii: 2

    Insert

    GBP nanobody
    

    Tags

    Trbo

    Expression

    Insect

    Promoter

    UAS-Gal4

    Available Since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDBTrp-LexABD-CRY2FL

    Plasmid

    #78210

    Purpose

    Use with Addgene 40615 for blue light transcription control in yeast

    Depositor

    Chandra Tucker

    Article

    Pathak et al ACS Synth Biol. 2014 Nov 21;3(11):832-8.

    Insert

    LexABD-CRY2 (CRY2 Mustard Weed)
    

    Tags

    lexA binding domain

    Expression

    Yeast

    Mutation

    contains endogenous CRY2 NLS

    Promoter

    ADH1

    Available Since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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