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We narrowed to 7,057 results for: crispr cas9 plasmids

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Showing: 381 - 400 of 7057 results

  1. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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  3. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available Since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

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  4. OmEF1aCas9P2ApuroSB

    Plasmid
    #165484
    Purpose
    Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells
    Depositor
    Dietmar Kueltz
    Article
    Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10
    Insert
    Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)
    Use
    CRISPR; Transposon
    Promoter
    Oreochromis mossambicus EF1 alpha promoter (OmEF1…
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    165484-plasmid-map-sequence-id-330622

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  5. pCas9GG

    Plasmid
    #131009
    Purpose
    Backbone plasmid for generating CRISPR arrays for SpCas9 using CRATES. Contains a direct repeat and a RFP-dropout cassette.
    Depositor
    Chase Beisel
    Article
    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:
    Inserts
    direct repeat of SpCas9
    promoter PJ23119
    mRFP expression cassette
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Bba_R0040 TetR
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    131009-plasmid-map-sequence-id-257082

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  8. pCMV-T7-SpCas9-P2A-EGFP (RTW3027)

    Plasmid
    #139987
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139987-plasmid-map-sequence-id-275335

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  9. IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

    Plasmid
    #102245
    Purpose
    Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.
    Depositor
    Jacob Corn
    Insert
    dCas9-VPR-mCherry
    Use
    CRISPR and Lentiviral
    Tags
    NLS-VPR-mCherry
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102245-plasmid-map-sequence-id-354473

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  10. lenti-EF1a-dCas9-KRAB-MeCP2-H2B-GFP

    Plasmid
    #242170
    Purpose
    Lentiviral expression of dCas9-KRAB-MeCP2 with H2B-GFP reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB-MeCP2; T2A-H2B-GFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Jan. 2, 2026
    Availability
    Academic Institutions and Nonprofits only
    242170-plasmid-map-sequence-id-496072

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  11. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

    Plasmid
    #192666
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192666-plasmid-map-sequence-id-379913

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  12. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192665
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available Since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192665-plasmid-map-sequence-id-379871

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  13. lenti-EF1a-dCas9-KRAB-MeCP2-H2B-mCherry

    Plasmid
    #242171
    Purpose
    Lentiviral expression of dCas9-KRAB-MeCP2 with H2B-mCherry reporter
    Depositor
    Amy Brock
    Insert
    dCas9-KRAB-MeCP2; T2A-H2B-mCherry
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Available Since
    Feb. 5, 2026
    Availability
    Academic Institutions and Nonprofits only
    242171-plasmid-map-sequence-id-499156

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  15. pJEP315-pAAV-U6SaCas9gRNA(CREB)-CMV-SaCas9-DIO-pA

    Plasmid
    #113692
    Purpose
    U6 SaCas9 gRNA expression cassette containing a gRNA targeting the CREB gene, followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.
    Depositor
    Jonathan Ploski
    Article
    Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.
    Inserts
    SaCas9 (NEWENTRY )
    SaCas9 gRNA Cassette (NEWENTRY )
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    NLS
    Promoter
    Cytomegalo Virus(CMV)
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    113692-plasmid-map-sequence-id-229557

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  16. SP6-hCas9-Ce-mRNA

    Plasmid
    #47911
    Purpose
    In vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.
    Depositor
    Paul Sternberg
    Article
    Chiu et al Genetics. 2013 Aug 26.
    Insert
    hCas9 with C. elegans 5' and 3' UTRs
    Use
    CRISPR
    Promoter
    SP6
    Available Since
    Sept. 13, 2013
    Availability
    Academic Institutions and Nonprofits only
    47911-plasmid-map-sequence-id-68528

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Showing: 381 - 400 of 7057 results