We narrowed to 1,383 results for: piggyBac

1. PB-SRT-Puro_BC3

   Plasmid

   #193145

   Purpose

   Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

   Depositor

   Rob Mitra

   Article

   Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

   Insert

   Barcoded piggyBac Puro SRT
   

   Expression

   Mammalian

   Mutation

   Barcode: CCAA

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-SRT-Puro_BC2

   Plasmid

   #193144

   Purpose

   Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

   Depositor

   Rob Mitra

   Article

   Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

   Insert

   Barcoded piggyBac Puro SRT
   

   Expression

   Mammalian

   Mutation

   Barcode: CTGA

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PB-SRT-Puro_BC1

   Plasmid

   #193143

   Purpose

   Barcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

   Depositor

   Rob Mitra

   Article

   Lalli et al NAR Genom Bioinform. 2022 Aug 31;4(3):lq

   Insert

   Barcoded piggyBac Puro SRT
   

   Expression

   Mammalian

   Mutation

   Barcode: CACA

   Available Since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLB37_PB_pGK-H2B-mIFP-T2A-rTetR-HDAC4-zeo

   Plasmid

   #179440

   Purpose

   Expresses rTetR-HDAC4(human) for gene silencing, integration by Piggybac

   Depositor

   Lacra Bintu

   Article

   Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1

   Insert

   H2B-mIFP-T2A-rTetR-HDAC4
   

   Expression

   Mammalian

   Promoter

   pGK

   Available Since

   Feb. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TAC-OKMS-KRAB-dCas9

   Plasmid

   #120356

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. BII-ChPtW-tdT

   Plasmid

   #133401

   Purpose

   Piggybac vector for constitutive expression of tdTomato

   Depositor

   Blair Madison

   Insert

   tdTomato
   

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CMV enhancer and hEf1a (CpG free)

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-CRX-T2A-NeuroD1

   Plasmid

   #194607

   Purpose

   Piggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.

   Depositor

   Haruhisa Inoue

   Article

   Otsuka et al iScience. 2022 Mar 8;25(4):103987. doi:

   Insert

   CRX-T2A-NeuroD1
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   tetO

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBRPB CAG-mCherry-IP

   Plasmid

   #106333

   Purpose

   PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

   Depositor

   Thomas Tuschl

   Article

   Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

   Insert

   mCherry-IRES-Puro
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB9v1

   Plasmid

   #210028

   Purpose

   Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   Cas9
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS and nucleoplasmin NLS

   Expression

   Mammalian

   Promoter

   EF-1α and TRE3G

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TO-NFIA.1-SOX9 (bi-directional promoter)

    Plasmid

    #182306

    Purpose

    Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Inserts

    NFIA (NFIA Human)
    SOX9 (SOX9 Human)
    puroR-T2A-mycNLS-mTagBFP2
    

    Use

    Piggybac

    Tags

    None and T2A-mycNLS-mTagBFP2

    Expression

    Mammalian

    Promoter

    TRE3G-Bi-directional

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-5'-ENGRAM

    Plasmid

    #179157

    Purpose

    piggyBac plasmid for 5' ENGRAM recorder

    Depositor

    Jay Shendure

    Article

    Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    minP

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 138-dCas9-Dnmt3a

    Plasmid

    #84570

    Purpose

    PiggyBac transposon system construct with dCas9-Dnmt3a

    Depositor

    Rudolf Jaenisch

    Article

    Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

    Insert

    dCas9-Dnmt3a (DNMT3A Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLR5-CAG-hMCP-mScarlet-I-NLS

    Plasmid

    #174878

    Purpose

    PiggyBac transposon vector for expression of MCP-mScarlet-I-NLS

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    MCP-mScarlet-I-NLS
    

    Use

    Piggybac vector

    Expression

    Mammalian

    Available Since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-SRT-tdTomato

    Plasmid

    #154885

    Purpose

    piggyBac self-reporting transposon with tdTomato marker for mammalian calling cards

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    piggyBac SRT
    

    Use

    Transposon

    Expression

    Mammalian

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB-SRT-Puro

    Plasmid

    #154884

    Purpose

    piggyBac self-reporting transposon with puromycin marker for mammalian calling cards

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    piggyBac SRT
    

    Use

    Transposon

    Expression

    Mammalian

    Available Since

    July 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCh-LgBiT-2A-EGFP-Vin-PILATeS

    Plasmid

    #216761

    Purpose

    PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposase

    Depositor

    Alex Dunn

    Article

    Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

    Insert

    mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
    

    Tags

    EGFP and mCherry

    Expression

    Mammalian

    Available Since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPB1163

    Plasmid

    #210032

    Purpose

    Contains Cas3 gRNA cloning site, inducible Cas11-P2A-Cas6-T2A-Cas3, rtTA-T2A-blast

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas11-P2A-Cas6-T2A-Cas3
    rtta-T2A-blast
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    EF-1α and TRE3G

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TAC-OK+9MS-Cas9

    Plasmid

    #120354

    Purpose

    piggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

    Depositor

    Knut Woltjen

    Article

    Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

    Insert

    OK+9MS cassette
    

    Use

    CRISPR; Piggybac transposon

    Expression

    Mammalian

    Mutation

    Klf4 [1-483]

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

    Plasmid

    #201831

    Purpose

    piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Inserts

    CAG
    BFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. XZ091 (PB) CMV-sCCL20 (PuroR-BFP)

    Plasmid

    #233434

    Purpose

    PiggyBac donor plasmid, secreted CCL20

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    CCL20 (CCL20 Human)
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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