We narrowed to 10,970 results for: T7

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pRSF-KpDcr1deltaC(E224Q, GiVL-2)

Plasmid

#32035

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Tags

His6-SUMO

Expression

Bacterial

Mutation

E224Q and N195-M215 replaced by P697-D714 of GiDi…

Promoter

T7

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 Cas2 E9Q

Plasmid

#80091

Purpose

Encodes Cas1 and Cas2 E9Q

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array (bp1542-2899)

Expression

Bacterial

Mutation

E9Q

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V134L Cas2

Plasmid

#80092

Purpose

Encodes Cas1 V134L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V134L

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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enz.043-SETD7-Y305F

Plasmid

#154045

Purpose

Expresses SETD7 (Y305F) in E. coli

Depositor

Christopher Voigt

Article

Wallace et al Advanced Functional Materials 2020

Insert

Histone-lysine N-methyltransferase SETD7 (SETD7 Human)

Tags

His, MBP

Expression

Bacterial

Mutation

changed tyrosine 305 to phenylalanine

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pET-29b(+)_NSP4ss_SerM_NucA-His

Plasmid

#218190

Purpose

Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Article

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

10xHis and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized for E. coli, residues 22-266

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b RNase A

Plasmid

#58903

Purpose

Bacterial expression of bovine pancreatic ribonuclease A

Depositor

Ronald Raines

Article

delCardayre et al Protein Eng. 1995 Mar;8(3):261-73.

Insert

RNase A (RNASE1 Bovine)

Tags

pelB sequence

Expression

Bacterial

Mutation

See Depositor Comments below

Promoter

T7

Available Since

Feb. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pFGET19_Ulp1

Plasmid

#64697

Purpose

H6-Ulp1 from pHYRS52 into pET-28b(+)

Depositor

Hideo Iwai

Article

Guerrero et al Protein Expr Purif. 2015 Aug 20. pii: S1

Insert

ULP1 (ULP1 Saccharomyces cerevisiae, Budding Yeast)

Tags

6xHis

Expression

Bacterial

Mutation

Ulp1(403-621)

Promoter

T7

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S2

Plasmid

#128590

Purpose

Expresses tagless S2 ribosomal protein in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S2 (rpsB E.coli)

Expression

Bacterial

Promoter

T7

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET-hisT4DNALig

Plasmid

#216671

Purpose

Expresses his-tagged T4 DNA ligase in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

T4 DNA Ligase (30 T4 phage)

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-GroEL/ES

Plasmid

#83922

Purpose

Co-expresses cytoplasmic copies of the Skp and Gro EL/ES protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Gro EL/ES

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pET-hisTdT

Plasmid

#216674

Purpose

Expresses his-tagged N-terminus truncated TdT in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

terminal deoxynucleotidyl transferase

Tags

his-tag

Expression

Bacterial

Mutation

1-138 truncation

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

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His-TEV-SNAP-PRC1

Plasmid

#167541

Purpose

For expression of His6-TEV-SNAP-PRC1 in bacteria.

Depositor

Tarun Kapoor

Article

Subramanian et al Cell. 2013 Jul 18;154(2):377-90. doi: 10

Insert

PRC1 (PRC1 Human)

Tags

His6 tag and SNAP tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pmRNA-EGFP-A120

Plasmid

#225903

Purpose

In vitro transcription of cap-dependently translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

A-inserted T7 class III Φ6.5 promoter
EGFP
2x β-globin UTR
poly(A) tail

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a_T7-ARG1

Plasmid

#106473

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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pET29-neoLux1.2

Plasmid

#229952

Purpose

Bacterial expression for de novo luciferase, neoLux1.2

Depositor

Andy Yeh

Article

Chen et al Chem. 2025 Mar 13;11(3):102346. doi: 10.

Insert

neoLux1.2

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b-6h-GST-TEVp

Plasmid

#172887

Purpose

Codon optimized (E. coli) TEVp fused with GST and his-tagged for purification

Depositor

Urartu Seker

Article

Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

Insert

TEV protease

Tags

GST solubility tag and HHHHHH

Expression

Bacterial

Promoter

T7 promoter

Available Since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP 150TAG

Plasmid

#85493

Purpose

pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP 150TAG

Expression

Bacterial

Mutation

150 TAG (amino acid 150 mutated to stop codon)

Promoter

T7

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET-mNeonGreen-HMG-15b

Plasmid

#206872

Purpose

Express mNeonGreen-HMG

Depositor

Kyubong Jo

Article

Kim et al Molecules. 2022 Aug 17;27(16):5248. doi:

Insert

mNeonGreen-HMG

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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pET15b-AavLEA1

Plasmid

#53093

Purpose

AavLEA1 expression (in BL21 DE3 E. coli strain)

Depositor

Claude Férec

Article

Tripathi et al PLoS One. 2014 Aug 13;9(8):e104970. doi:

Insert

AavLEA1

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

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