167,785 results

1. Dual sgRNA CRISPRi Library

   Pooled Library

   #1000000192

   Depositor

   Jonathan Weissman

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

2. pGL3 NSE promoter

   Plasmid

   #11606

   Depositor

   Rosalyn Adam

   Article

   Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

   Insert

   NSE promoter (ENO2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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3. pG2K

   Plasmid

   #71742

   Purpose

   Modular shuttle vector for Geobacillus and E. coli

   Depositor

   Tom Ellis

   Article

   Reeve et al ACS Synth Biol. 2016 Jul 1.

   Type

   Empty backbone

   Use

   Synthetic Biology; Shuttle vector

   Expression

   Bacterial

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti PGK Neo DEST (w531-1)

   Plasmid

   #19067

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

   Depositor

   Paul Kaufman, Eric Campeau

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. TRUPATH Triple Gai3

   Plasmid

   #196050

   Purpose

   Encodes a G alpha subunit (GNAl3) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai3-RLuc8 (GNAI3 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1+SKL

   Plasmid

   #53450

   Purpose

   expresses GFP targeted for localization in peroxisomes

   Depositor

   Jay Brenman

   Article

   Sexton et al Int J High Throughput Screen. 2010 Jul;2

   Insert

   SKL amino acids
   

   Tags

   SKL

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

7. E228Q Vps4A-HA

   Plasmid

   #200087

   Purpose

   3x HA-VPS4A with E228Q dominant negative mutation in Dox-inducible pInducer20 vector

   Depositor

   Jason MacGurn

   Article

   Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

   Insert

   VPS4A (VPS4A Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   E228Q

   Promoter

   CMV

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-Con/Foff 2.0-ChR2-mCherry (AAV8)

   Viral Prep

   #137143-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-nEF-Con/Foff 2.0-ChR2-mCherry (#137143). In addition to the viral particles, you will also receive purified pAAV-nEF-Con/Foff 2.0-ChR2-mCherry plasmid DNA. nEF-driven, Cre-dependent expression of ChR2-mCherry for optogenetic activation (inhibited in the presence of Flp recombinase). These AAV preparations are suitable purity for injection into animals.

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Promoter

   nEF

   Tags

   mCherry (Cre-dependent)

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

9. pcDNA3-MEK5DD-HA

   Plasmid

   #65247

   Purpose

   Expression of HA tagged dominant active MEK5 in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

   Insert

   MEK5DD (MAP2K5 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   changed Ser311 to Asp, Ser315 to Asp

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX208-cytoFLARE1.0-TF

    Plasmid

    #234513

    Purpose

    Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

    Depositor

    Wenjing Wang

    Article

    Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

    Insert

    ERT2-MK2-hLOV-TEVcs-NLS-GAL4-FLAG
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 3, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. mEmerald-Sec61b-C1

    Plasmid

    #90992

    Purpose

    Expresses mEmerald-tagged Sec61b, brightly labels endoplasmic reticulum in mammalian cells

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Nixon-Abell et al Science. 2016 Oct 28;354(6311). pii: aaf

    Insert

    mEmerald-Sec61b (SEC61B Human, Synthetic)
    

    Tags

    mEmerald (green fluorescence)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR221_SLC25A5

    Plasmid

    #132007

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    Giulio Superti-Furga, RESOLUTE Consortium

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC25A5 (SLC25A5 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 24, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pFastBac-Dual-M2

    Plasmid

    #135584

    Purpose

    High eukaryotic dual protein expression shuttle vector

    Depositor

    Xiao-Wen Cheng

    Article

    Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

    Type

    Empty backbone

    Use

    Transfer vector

    Expression

    Insect

    Promoter

    polyhedrin of AcMNPV

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-S5E2-ChR2-mCherry (AAV PHP.eB)

    Viral Prep

    #135634-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from pAAV-S5E2-ChR2-mCherry (#135634). In addition to the viral particles, you will also receive purified pAAV-S5E2-ChR2-mCherry plasmid DNA. Expression of ChR2-mCherry under the control of the E2 regulatory element. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Tags

    mCherry

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pAAV-Syn-Chronos-GFP (AAV1)

    Viral Prep

    #59170-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-Syn-Chronos-GFP (#59170). In addition to the viral particles, you will also receive purified pAAV-Syn-Chronos-GFP plasmid DNA. Syn-driven Chronos-GFP expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    GFP

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

16. pJT301 BxBi Lenti pEF-attB LSR GFP

    Plasmid

    #184944

    Purpose

    Lentiviral delivery of recombinase landing pad

    Depositor

    Patrick Hsu, Ami Bhatt, Michael Bassik, Lacra Bintu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    BxBi Lenti pEF1a-attB LSR GFP
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIRESneo-PA-GFP-alpha Tubulin

    Plasmid

    #12297

    Depositor

    Patricia Wadsworth

    Article

    Tulu et al Curr Biol. 2003 Oct 28. 13(21):1894-9.

    Insert

    alpha Tubulin (TUBA1B Human)
    

    Tags

    PA-GFP

    Expression

    Mammalian

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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18. pscAAV-GFP

    Plasmid

    #32396

    Depositor

    John T Gray

    Article

    Gray et al Methods Mol Biol. 2011;807:25-46.

    Insert

    GFP
    

    Use

    AAV; Adeno-associated virus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pInt_attP1_LCS_kanR

    Plasmid

    #205300

    Purpose

    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.

    Depositor

    Scott Saunders

    Article

    Saunders et al bioRxiv 2023

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    Sept. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLminP_Luc2P_RE4

    Plasmid

    #90338

    Purpose

    NRF2 - Antioxidant response element gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Promoter

    NRF2

    Available Since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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