239,785 results

1. pcDNA3.1.Gαs-LgB113

   Plasmid

   #134362

   Purpose

   Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   Gαs-LgB113 (GNAS Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-dual-CRISPR-U6-H1(pBP43)

   Plasmid

   #218930

   Purpose

   The backbone lentiviral dual-CRISPR plasmid for making the dual-CRISPR screening libraries.

   Depositor

   Baoxu Pang

   Article

   Li et al Nat Biomed Eng. 2024 May 22. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   human U6 and H1

   Available Since

   July 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rtn4a-mNeonGreen

   Plasmid

   #169032

   Purpose

   Express human Rtn4a

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rtn4a (RTN4 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GFP-IRES-hSNCA

   Plasmid

   #185715

   Purpose

   AAV expression of GFP and human α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLminP_Luc2P_RE34

   Plasmid

   #90376

   Purpose

   MAPK - Elk1 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   ELK1

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLminP_Luc2P_RE34

   Plasmid

   #90376

   Purpose

   MAPK - Elk1 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Promoter

   ELK1

   Available Since

   Aug. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Available Since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe K-Ras 12V

   Plasmid

   #12544

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   K-Ras 12V (KRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   12V constituitively activated

   Available Since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPLICSFAST-ER-MT-P2A

   Plasmid

   #214892

   Purpose

   Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-MT-P2A
   

   Tags

   none

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH-EF1-mVenus-p27K−

    Plasmid

    #176651

    Purpose

    It encodes an mVenus fused to a mutant p27K-, which lacks binding affinity to Cdk. This reporter helps to identify quiescent disseminated tumor cells (in G0 phase of cell cycle).

    Depositor

    Mohamed Bentires-Alj

    Article

    Correia et al Nature. 2021 Jun;594(7864):566-571. doi:

    Insert

    mVenus-p27K- (Cdkn1b Mouse)
    

    Use

    Lentiviral

    Tags

    mVenus

    Mutation

    Mutant K- (lacks affinity to Cdk)

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-EF1a ANXA11-mEmerald

    Plasmid

    #164210

    Purpose

    pLEX lentivirus backbone expresses mEmerald tagged ANXA11 under EF1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    ANXA11 (ANXA11 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Promoter

    EF1a

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. psgRNA_EcoWG1

    Pooled Library

    #131625

    Purpose

    Improved genome-wide knockdown of E. coli genes

    Depositor

    David Bikard

    Article

    Calvo-Villamanan et al Nucleic Acids Res. 2020 Apr 30. pii: 582

    Expression

    Bacterial

    Species

    Escherichia coli

    Use

    CRISPR

    Available Since

    May 12, 2020

    Availability

    Academic Institutions and Nonprofits only

13. Tet-O-FUW-Neurod1

    Plasmid

    #30129

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    NeuroD1 (Neurod1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet-O-FUW-Neurod1

    Plasmid

    #30129

    Depositor

    Marius Wernig

    Article

    Vierbuchen et al Nature. 2010 Feb 25. 463(7284):1035-41.

    Insert

    NeuroD1 (Neurod1 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. SPLICS Po-ER Long P2A

    Plasmid

    #164113

    Purpose

    Detect the long Peroxisomes-Endoplasmic reticulum contact

    Depositor

    Marisa Brini, Tito Calì

    Article

    Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

    Insert

    Split YFP Po-ER Long P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. mCherry-Golgi-7

    Plasmid

    #55052

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Golgi (B4GALT1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. mCherry-Golgi-7

    Plasmid

    #55052

    Purpose

    Localization: Golgi Complex, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Golgi (B4GALT1 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB-TRE3G-MYCN

    Plasmid

    #104542

    Purpose

    PiggyBac vector encoding doxycycline-inducible human MYCN

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
    

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TRE3G-MYCN

    Plasmid

    #104542

    Purpose

    PiggyBac vector encoding doxycycline-inducible human MYCN

    Depositor

    David Vereide

    Article

    Miller et al Stem Cell Reports. 2018 Jan 9;10(1):73-8

    Insert

    MYCN proto-oncogene, bHLH transcription factor (MYCN Human)
    

    Expression

    Bacterial and Mammalian

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF)

    Pooled Library

    #1000000113

    Purpose

    Genome-wide CRISPR gRNA pooled library for activation of mouse genes

    Depositor

    David Root, John Doench

    Available Since

    July 13, 2018

    Availability

    Academic Institutions and Nonprofits only