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pBABE hygro MEN1 A242V

Plasmid

#11022

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 A242V (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

A242V

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pKL038 Lenti U6 dCas12a gRNA Puro mCherry

Plasmid

#195546

Purpose

dCas12a gRNA expression backbone

Depositor

Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Lenti U6- empty cassette_Direct repeat_puro_mcherry

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBpaRS3tRNA

Plasmid

#197102

Purpose

To express the p-benzoylphenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into p-benzoylphenylalanine.

Depositor

Kensaku Sakamoto

Article

Sato et al Biochemistry. 2011 Jan 18;50(2):250-7. d

Insert

TyrRS variant, amber suppressor tRNA

Expression

Bacterial

Mutation

Gly32, Ser107, Thr158, Ser159, Arg286 (TyrRS)

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-5HRE-GFP NeoR

Plasmid

#128960

Purpose

Lentiviral plasmid for expression of hypoxia-responsive enhanced green fluorescent protein

Depositor

Carlos Carmona-Fontaine

Article

Janska et al Dis Model Mech. 2021 Aug 1;14(8). pii: 2

Insert

5X HRE of VEGF (VEGFA Human)

Use

Lentiviral

Tags

d2EGFP

Expression

Mammalian

Promoter

minimal CMV promoter

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP10_nostop

Plasmid

#149313

Purpose

Gateway-compatible Entry vector, with insert of NSP10 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP10 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 SARS-CoV-2 NSP4_nostop

Plasmid

#149307

Purpose

Gateway-compatible Entry vector, with insert of NSP4 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP4 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGLUE-SHLD2

Plasmid

#114118

Purpose

Expresses N-terminally tagged Streptag-HA-CBP-SHLD2 in mammalian cells

Depositor

Daniel Durocher

Article

Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

Insert

SHLD2 (SHLD2 Human)

Tags

HA tag, Streptavidin-binding tag (Streptag), and …

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pSICO-CPSF6-358-eGFP

Plasmid

#110693

Purpose

Expresses a truncated form of CPSF6 (CPSF6-358 originally described in Lee et al. Cell Host Microbe 2010) fused with eGFP

Depositor

Zandrea Ambrose

Article

Ning et al J Virol. 2018 Apr 11. pii: JVI.00368-18.

Insert

CPSF6-eGFP (CPSF6 )

Use

Lentiviral

Tags

EGFP

Mutation

Truncation of CPSF6 isoform 2 at amino acid 358

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Ywhag CDS

Plasmid

#61799

Purpose

pcDNA3.1(+) with full length mouse Ywhag CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Ywhag CDS (Ywhag Mouse)

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pBABE-puro-NLS-LSSmKate1

Plasmid

#31873

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

NLS-LSS-mKate1

Use

Retroviral

Expression

Mammalian

Mutation

K69Y/P131T/S148G/M167E/T183S/M196V compared to mK…

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJA4

Plasmid

#19494

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA4 (DNAJA4 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pGFP-mHP1alpha

Plasmid

#181900

Purpose

Fluorescently tagged HP1alpha

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

mHP1alpha (Cbx5 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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GST-GGA3-VHS-GAT

Plasmid

#173719

Purpose

VHS-GAT domain in GGA3 can bind to an active form of ARF1 protein. Using this construct, you can assess the activity of ARF1 protein.

Depositor

Juan Bonifacino

Article

Dell'Angelica et al J Cell Biol. 2000 Apr 3;149(1):81-94. do

Insert

GGA3 (GGA3, GGA2, GGA1 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSKC12 - DelQ

Plasmid

#110493

Purpose

Monitor the expression of Luciferase driven by a mutated NRAS 5'-UTR.

Depositor

Shankar Balasubramanian

Article

Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

Insert

NRAS 5'- DelQ (bp 30-254) (NRAS Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Deletion of the first 29 base pairs of the NRAS 5…

Promoter

T7

Available Since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG-AILR-LplA-ER

Plasmid

#42574

Depositor

Alice Ting

Article

Liu et al ACS Nano. 2012 Dec 21;6(12):11080-7. doi

Insert

LplA(AILR) with ER-retention sequence KDEL

Tags

FLAG

Mutation

Compared to wild-type LplA: tryptophan 37 to alan…

Promoter

CAG

Available Since

April 11, 2013

Availability

Academic Institutions and Nonprofits only
pRGEN-CMV-VPR-L1-CjCas9 V-D

Plasmid

#169913

Purpose

Expression of a VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn-HpaBC

Plasmid

#100949

Purpose

Expression of RgTALsyn, HpaB, and HpaC from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Inserts

RgTALsyn
HpaB
HpaC

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-P2A-CIAR_pLenti_CMV_Puro

Plasmid

#86503

Purpose

Lenti plasmid for generating CIAR expressing stable cell lines

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

GFP-P2A-CIAR (SOS1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

T968L in SOScat

Promoter

CMV

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-CohIII-CBM-HIS-ybbR

Plasmid

#60866

Purpose

Characteristic fingerprint molecule fused to cohesin type III domain to perform single molecule force spectroscopy experiments; covalently immobilized via introduced ybbR tag

Depositor

Hermann Gaub

Article

Schoeler et al Nat Commun. 2014 Dec 8;5:5635. doi: 10.1

Inserts

CohIII
CBM

Tags

6xHIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

Cysteine 63 to Serine in CBM module

Promoter

T7

Available Since

Nov. 26, 2014

Availability

Academic Institutions and Nonprofits only

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