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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-GlnRS-His

Plasmid

#124108

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Glutaminyl-tRNA ligase (glnS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-His-IleRS

Plasmid

#124112

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Isoleucyl-tRNA ligase (ileS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG.PKCzeta.K/W

Plasmid

#10800

Depositor

Alex Toker

Article

Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

Insert

PKC zeta (Prkcz Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Kinase dead mutant: K281W (Lys 281 to Trp)

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only
HA-Daxx/pRK5

Plasmid

#52023

Purpose

Expresses HA-Daxx in mammalian cells

Depositor

Xiaolu Yang

Article

Tang et al PLoS One. 2013;8(2):e55813. doi: 10.1371

Insert

HA-Daxx (DAXX Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX6p1-hPdcd4

Plasmid

#20693

Depositor

Haiwei Song

Article

Loh et al EMBO J. 2009 Feb 4. 28(3):274-85.

Insert

Programmed Cell Death Protein 4 (PDCD4 Human)

Tags

GST

Expression

Bacterial

Mutation

I36V- does not alter function

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only
pINDUCER-21-RUNX1

Plasmid

#97043

Purpose

Expresses RUNX1 in mammalian cells

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

Tet on

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pCDNA3-STAT3-S727D

Plasmid

#73364

Purpose

Expression of STAT3-S727D

Depositor

Jie Chen

Article

Kim et al J Biol Chem. 2009 Dec 18;284(51):35425-3

Insert

Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)

Tags

FLAG tagged

Expression

Mammalian

Mutation

E16K S727D

Promoter

CMV

Available Since

May 23, 2016

Availability

Academic Institutions and Nonprofits only

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piCRg Entry

Plasmid

#58904

Purpose

Cas9/gRNA Entry expression plasmid

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pLB2 CAG P2Gm

Plasmid

#19752

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Type

Empty backbone

Use

Lentiviral, Mouse Targeting, and RNAi

Expression

Mammalian

Available Since

Feb. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Foxp1A

Plasmid

#16362

Depositor

Anjana Rao

Article

Hu et al Nat Immunol. 2006 Aug . 7(8):819-26.

Insert

FOXP1A (Foxp1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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FBP17-pmCherryC1

Plasmid

#27688

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

FBP17 (FNBP1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
pENTR-3xFLAG-YAP1-S127A

Plasmid

#42239

Depositor

William Pu

Article

von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

Insert

YAP1 (YAP1 Human)

Use

Gateway Cloning

Tags

3xFLAG

Mutation

Serine 127 changed to alanine

Promoter

none

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v4-BFP-24xPP7

Plasmid

#74929

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Article

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-BFP-24xPP7

Expression

Mammalian

Available Since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSuperRetro control

Plasmid

#11189

Depositor

Bruce Spiegelman

Article

Drori et al Genes Dev. 2005 Feb 1. 19(3):362-75.

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pAAV-CaMKII-Chronos-GFP

Plasmid

#58805

Purpose

AAV expression of Chronos-GFP under the CaMKII promoter

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CaMKII

Available Since

Aug. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pBabe.puro.CDK8.flag

Plasmid

#19758

Depositor

William Hahn

Article

Firestein et al Nature. 2008 Sep 25. 455(7212):547-51.

Insert

cyclin-dependent kinase 8 (CDK8 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Dec. 11, 2008

Availability

Academic Institutions and Nonprofits only

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hMEF2A WT

Plasmid

#118354

Purpose

This plasmid expresses human MEF2A-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pSADdeltaG-mCherry

Plasmid

#32636

Depositor

Edward Callaway

Article

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Insert

mCherry

Use

Rabies virus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

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pQP-T2A

Plasmid

#102583

Purpose

Light-controlled activation of expression

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Chem Biol. 2017 Mar 27. doi: 10.1038

Insert

BphP1-VP16-T2A-NLS-GAL4-QPAS1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Academic Institutions and Nonprofits only

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