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SPOP-V5 (W131G)

Plasmid

#169724

Purpose

Mammalian Expression of mouse SPOP W131G

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

W131G

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-CaMKKbeta

Plasmid

#32453

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Tags

FLAG and GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

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KP501: pMVP (L5-L4) eYFP-P2A

Plasmid

#121714

Purpose

pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

eYFP-P2A (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LC001: pMVP/PB/Hygro-DEST

Plasmid

#121876

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LB601: pMVP/PB/eGFP-DEST

Plasmid

#121870

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pYpet-PBD

Plasmid

#22781

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

PAK binding domain (PAK1 Human)

Tags

Ypet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pFloat.T7_v1

Plasmid

#133070

Purpose

Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGLOW77

Plasmid

#177338

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-2

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-Flag-RNF169

Plasmid

#74243

Purpose

Expresses RNF169 in mammalian cells

Depositor

Daniel Durocher

Article

Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

Insert

RNF169 (RNF169 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pBS-KS-attB2-SA(0)-T2A-LexA::QFAD-Hsp70

Plasmid

#62947

Purpose

Creating LexA::QF drivers from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-LexA::QFAD-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-Gal4-Hsp70

Plasmid

#62896

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 0.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1

Plasmid

#109581

Purpose

Multisite gateway vector for 3' tagging with Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pC-(loxP2-attB2-SA(2)-T2A-Gal4-Hsp70)

Plasmid

#62956

Purpose

Singlet donor (phase 2) for in vivo RMCE with MiMIC inserts to make Gal4 driver.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4-Hsp70

Available Since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-CMV-TetO2_EmGFP

Plasmid

#113892

Purpose

Mammalian (inducible) expression of cytosolic EmGFP

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

Emerald GFP

Use

Lentiviral

Promoter

CMV-MIE-TetO2

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCyPet-Rac1(WT)

Plasmid

#22782

Depositor

Klaus Hahn

Article

Machacek et al Nature. 2009 Sep 3. 461(7260):99-103.

Insert

Rac1 (wt) (RAC1 Human)

Tags

CyPet

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pRSF-G1(7AW)RS

Plasmid

#207516

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Article

Abdelkader et al ACS Sens. 2023 Oct 27. doi: 10.1021/acss

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pGLOW31

Plasmid

#172698

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-3::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-3

Available Since

Aug. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAVf-EnhCB-lacZnls miR-122 3x

Plasmid

#35644

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Insert

3 miR-122 target sites

Use

AAV

Promoter

CB

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyn-PlcR(K30/32L)

Plasmid

#181831

Purpose

Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

Depositor

Jin Zhang

Article

Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

Insert

Lyn-PlcR (K30L,K32L)

Tags

ECFP, EYFP, and N-terminal targeting sequence fro…

Expression

Mammalian

Mutation

Lysines 30 and 32 in PH domain mutated to leucine.

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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SPOP-V5 (BACK))

Plasmid

#169722

Purpose

Mammalian Expression of mouse SPOP Y353E

Depositor

Tanja Mittag

Article

Bouchard et al Mol Cell. 2018 Oct 4;72(1):19-36.e8. doi

Insert

SPOP (Spop Mouse)

Tags

V5

Expression

Mammalian

Mutation

Y353E

Promoter

CMV

Available Since

June 14, 2021

Availability

Academic Institutions and Nonprofits only

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