We narrowed to 7,663 results for: ski

1. pAAV-CaMKII-iGECI

   Plasmid

   #160423

   Purpose

   AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI

   Depositor

   Vladislav Verkhusha

   Article

   Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

   Insert

   iGECI
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Nov. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEND-193_Cacnes_HS

   Plasmid

   #225622

   Purpose

   C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   HspR
   

   Use

   Synthetic Biology

   Tags

   pFAST-6XHis

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. miniABEMax V82G wt

   Plasmid

   #135362

   Purpose

   Expression of nSpyCas9 with N-terminal miniABEMax (V82G)

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax nSpyCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEND-303_Cacnes_tetR

   Plasmid

   #225618

   Purpose

   C. acnes with tetR-based inducible expression of mCherry. pBRESP36A with P(PPA_RS09745)+RBS_2+TetR // P(BBa_23119-TetO2)+RBS_1+mCherry

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   TetR
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEND-192_Cacnes_HS

   Plasmid

   #225621

   Purpose

   C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS09160)+RBS_1+HspR(PPA_RS10230).

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   HspR
   

   Use

   Synthetic Biology

   Tags

   pFAST-6XHis

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX Flag RagC W115R

   Plasmid

   #112759

   Purpose

   express Flag-RagC (W115R mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-RagC (W115R) (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   W115R

   Available Since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. HA-MBP-TEVpCS-ADGRG6 CTF-FLAG

   Plasmid

   #217704

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGR6 (ADGRG6 Human)
   

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-840

   Available Since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLVX Flag RagC T90N

   Plasmid

   #112758

   Purpose

   express Flag-RagC (T90N mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   Flag-RagC (T90N) (RRAGC Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Mutation

   T90N

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3.0_mitoLAMA-F98 IRES ShadowG-mScarlet

   Plasmid

   #130708

   Purpose

   Plasmid encoding outer mitochondrial localization of LAMA-F98 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   NTOM20-SNAP-Tag-LAMA-F98 IRES ShadowG-mScarlet
   

   Tags

   NTOM20 and SNAP-Tag

   Expression

   Mammalian

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX Stat5A-SH2

    Plasmid

    #46515

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat5A (STAT5A Human)
    

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 582-693

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHS1861

    Plasmid

    #205974

    Purpose

    FLAG-SV40 NLS-Cas8-HNH from JAGBLH010000220 Cas8-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH
    

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-MBP-TEVpCS-ADGRL2 CTF-FLAG

    Plasmid

    #217707

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRL2 (ADGRL2 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-824

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEX-5X1 Flag hDPY30

    Plasmid

    #15556

    Depositor

    Kai Ge

    Article

    Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

    Insert

    DPY30 (DPY30 Human)
    

    Tags

    Flag and GST

    Expression

    Bacterial

    Available Since

    Aug. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pGEX Stat5B-SH2

    Plasmid

    #46516

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat5B (STAT5B Human)
    

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 593-692

    Promoter

    Tac

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDule2-IBBN (G2)

    Plasmid

    #85501

    Purpose

    Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

    Insert

    IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32G L65E F108W Q109M D158S R162K

    Promoter

    lpp (constitutive)

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. PVT1-luciferase

    Plasmid

    #113350

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    PVT1-enhancer (PVT1 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEY26

    Plasmid

    #191031

    Purpose

    cho-1(3.3-2.6k) fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    cho-1(3.3-2.6k) (cho-1 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEY39

    Plasmid

    #191043

    Purpose

    acr-2(2.1k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(2.1k) (acr-2 Nematode)
    

    Expression

    Worm

    Mutation

    None

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-flex-GFP-shRNA-scramble

    Plasmid

    #182503

    Purpose

    Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter

    Depositor

    William Wisden

    Article

    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.

    Insert

    pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
    

    Use

    AAV, Cre/Lox, and RNAi

    Expression

    Mammalian

    Promoter

    hSyn promoter

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCL-HA-MAPKK1-G1C [delta(44-51)/S218E/S222D]

    Plasmid

    #40807

    Depositor

    Natalie Ahn

    Article

    Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta(44-51), S218E, S222D

    Promoter

    CMV

    Available Since

    Oct. 18, 2012

    Availability

    Academic Institutions and Nonprofits only