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pAV-U6+27-Linear-1_PolyU(7)_Tornado-Corn

Plasmid

#159485

Purpose

Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(7)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(8)_Tornado-Corn

Plasmid

#159486

Purpose

Tests for the impact of 8 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(8)

Expression

Mammalian

Promoter

U6-27

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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CL20_MSCV-NUP98::PRRX1-ires-GFP

Plasmid

#237492

Purpose

Express NUP98::PRRX1 in bicistronic retroviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

NUP98::PRRX1 (PRRX1, NUP98 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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CCLMPC_HA-NUP98::PRRX1

Plasmid

#237493

Purpose

Express HA-tagged NUP98::PRRX1 in dual promoter lentiviral vector

Depositor

Charles Mullighan

Article

Michmerhuizen et al Cancer Discov. 2025 Jun 19. doi: 10.1158

Insert

HA-NUP98::PRRX1 (NUP98, PRRX1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MND/PGK

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV(CAT)T7-SB100

Plasmid

#34879

Depositor

Zsuzsanna Izsvak

Article

Mates et al Nat Genet. 2009 Jun;41(6):753-61. Epub 2

Insert

SB100X transposase

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pHES830

Plasmid

#87944

Purpose

Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

Zif268 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)

Expression

Yeast

Promoter

ADH1 (crippled)

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHES941

Plasmid

#87942

Purpose

Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Gal4 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

GAL4 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)

Expression

Yeast

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Neo-PIP-FUCCI

Plasmid

#118616

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

hPGK

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pSL2621 (pCas8_Vch)

Plasmid

#200893

Purpose

Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas8

Tags

NLS

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2623 (pCas6_Vch)

Plasmid

#200895

Purpose

Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas6

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2622 (pCas7_Vch)

Plasmid

#200894

Purpose

Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas7

Tags

NLS

Expression

Mammalian

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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612 p3E-2A-mCherryCAAXpA

Plasmid

#195971

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-mCherry-CAAX (membrane)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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458 p3E-2A-EGFP-CAAXpA

Plasmid

#195968

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP-CAAX (membrane)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEFBOS_CreIRESpuro

Plasmid

#183812

Purpose

Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

Depositor

Ed Stanley

Article

Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

Insert

Cre recombinase

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHREAC

Plasmid

#134908

Purpose

Overexpression of recombinant proteins in plants thanks to very efficient translational efficiency. Empty vector, clone ORF using Bsa1 sites. Download fully annotated map under Resource Information.

Depositor

George Lomonossoff

Article

Peyret et al Plant Methods. 2019 Sep 18;15:108. doi:

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

double 35S

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_CD8alpha SNIPR

Plasmid

#188357

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_CD8alpha-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_Hinge Notch SNIPR (LaG17)

Plasmid

#188380

Purpose

Lentiviral vector - constitutive expression of an anti-GFP SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiGFP_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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L_NLS 41kDa

Plasmid

#201342

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A4-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHDM-Tat1b

Plasmid

#164442

Purpose

Encodes HIV-1 Tat accessory protein for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

HIV-1 Tat

Expression

Mammalian

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer20-HA-TEADiv2

Plasmid

#228877

Purpose

Tet-inducible lentiviral vector for expression of optimized 2xHA-TEAD inhibitor (TEADiv2)

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

TEADiv2

Use

Lentiviral

Tags

2xHA

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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