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458 p3E-2A-EGFP-CAAXpA

Plasmid

#195968

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP-CAAX (membrane)

Use

Other

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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YFVdelNS1-mCherry

Plasmid

#179951

Purpose

pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

YFV-17D lacking nonstructural protein 1 (NS1) but with mCherry

Use

Transneuronal tracing

Promoter

SP6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLTR.gp140/EGFP.Revdelta38/DsRed

Plasmid

#115775

Purpose

HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

Depositor

Damian Purcell

Article

Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

Insert

Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).

Tags

EGFP; dsReD

Expression

Mammalian

Mutation

gp140 uncleaved (1190-91aa, KR > TG; truncated…

Promoter

HIV-1 (LTR)

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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M_NLS 41kDa

Plasmid

#201341

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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TPST1-EGFP

Plasmid

#66617

Purpose

Encodes GFP fusion of TPST1 trans Golgi marker; (Tyrosyl protein sulfotransferase 2)

Depositor

David Stephens

Article

Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

Insert

TPST1 (TPST1 Human)

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pHAGE-3FLAG-RAB5A

Plasmid

#176488

Purpose

For lentiviral transduction of 3FLAG-RAB5A

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

RAB5A (RAB5A Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD

Plasmid

#188374

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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YFVdelNS1-mVenus

Plasmid

#179950

Purpose

pCCI vector encoding YFV-17D lacking nonstructural protein 1 (NS1) but with mVenus

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

YFV-17D lacking Nonstructural protein 1 (NS1) but with mVenus

Use

Transneuronal tracing

Promoter

SP6

Available Since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-SNAP

Plasmid

#174879

Purpose

PiggyBac transposon vector for expression of NLS-SNAP

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

NLS-SNAP

Use

Piggybac vector

Expression

Mammalian

Available Since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pSL0341 (pTarget)

Plasmid

#200906

Purpose

Encodes an mCherry casette under a minimal CMV promoter. Used as the target plasmid for plasmid-based integration assays, and for plasmid-based transcriptional activation.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

pTarget

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_ALPPL2 Hinge Notch SNIPR

Plasmid

#188377

Purpose

Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

Plasmid

#137827

Purpose

MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

Depositor

Joshua Leonard

Article

Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

Insert

FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pHES830

Plasmid

#87944

Purpose

Constitutively expresses progesterone-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

Zif268 DBD - hPR LBD - MSN2 AD (PGR Synthetic, Human, Budding Yeast)

Expression

Yeast

Promoter

ADH1 (crippled)

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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NLS-dCas9-HA-NLS-GFP-VP16

Plasmid

#183923

Purpose

VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

dCas9-GFP-VP16

Use

CRISPR

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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Diffusive 27kDa (EGFP)

Plasmid

#201362

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP

Expression

Mammalian

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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Diffusive 34kDa

Plasmid

#201334

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-1PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-3HA

Plasmid

#67616

Purpose

A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

three inframe 3' HA tags

Expression

Mammalian

Promoter

CMV and SP6

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pBTK620

Plasmid

#110620

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Jan. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK621

Plasmid

#110621

Purpose

BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Expression

Bacterial

Available Since

Sept. 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pBTK519

Plasmid

#110603

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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