We narrowed to 31,602 results for: REP

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Lenti-eCas9-sgMIB1

Plasmid

#140239

Purpose

Lentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

sgRNA targeting human MIB1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHR LAT-FFF/Zap70 iLID-Drop

Plasmid

#171032

Purpose

Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.

Depositor

Jared Toettcher

Article

Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

Insert

LAT (LAT Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pscAAV-Tyr

Plasmid

#135616

Purpose

Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-Gal4

Plasmid

#62900

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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PXPR007 sgGFP-1

Plasmid

#74960

Purpose

Cas9 + sgGFP-1 with blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

GFP

Use

CRISPR

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-Gal4

Plasmid

#62901

Purpose

Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-Gal4

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3_altPKA-Cα-mEGFP

Plasmid

#114132

Purpose

Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

18747 (Prkaca Mouse)

Expression

Mammalian

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGL4.10-VEGFprom(-500 to -1)

Plasmid

#66130

Purpose

Luciferase-based reporter for VEGF promoter region (-500 to -1 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)

Use

Luciferase

Promoter

VEGF -500 to -1

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-CAGGS-Flex/5'USS-GFP

Plasmid

#197884

Purpose

Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

GFP

Use

AAV

Mutation

N/A

Promoter

CAG

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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Bxb1_miRFP703

Plasmid

#183759

Purpose

Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

miRFP703

Tags

Lck lipidation signal

Expression

Mammalian

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

Plasmid

#174891

Purpose

amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

Plasmid

#120269

Purpose

Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

Depositor

Edward Callaway

Article

Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

Insert

eSyn-DIO-TVA950:YFP

Use

AAV

Expression

Mammalian

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-5102HRasCT(Picchu)

Plasmid

#209917

Purpose

A FRET biosensor for tyrosine kinase activity

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

5102HRasCT

Tags

YPet and nTurquoise-GL

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pME18ST-d5-HT7

Plasmid

#53111

Purpose

Expression of Drosophila 5-HT7

Depositor

Leslie Vosshall

Article

Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

Insert

5HT7 (5-HT7 Fly)

Available Since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pGL4.10-VEGFprom(-1000 to -500)

Plasmid

#66129

Purpose

Luciferase-based reporter for VEGF promoter region (-1000 to -500 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)

Use

Luciferase

Promoter

VEGF -500 to -1

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-848-952(pL91)

Plasmid

#124388

Purpose

E.Coli produced TET1-848-952 purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-848-952 wildtype (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pSico-shRaptor

Plasmid

#81086

Purpose

conditional expression of an shRNA targeting murine Raptor

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

Raptor shRNA (Rptor Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pSCALPS-mAgo2-EGFP

Plasmid

#209935

Purpose

Lentiviral expression of mouse Argonaute-2 in mammalian cells

Depositor

Phillip Zamore

Article

Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

Insert

Ago2 (Ago2 Mouse)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-PH-SWAP70

Plasmid

#84582

Purpose

Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

Depositor

Geert van den Bogaart

Article

Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

Insert

SWAP70 residues 209-306 (SWAP70 Human)

Tags

mCherry

Expression

Mammalian

Mutation

Pleckstrin-homology domain, residues 209-306

Promoter

CMV

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

Plasmid

#220224

Purpose

AAV-STARR-seq validation control vector with Venus

Depositor

Simon Rumpel

Article

Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

Type

Empty backbone

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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