We narrowed to 15,215 results for: Rras

1. pAdSh.U6.gRNAS1

   Plasmid

   #58252

   Purpose

   U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   U6.gRNAS1 cassette
   

   Use

   Adenoviral and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLmoCascade-IL1RNcrRNA

   Plasmid

   #126487

   Purpose

   Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   Lmo IL1RN cr03
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TF-bio-His

   Plasmid

   #52184

   Purpose

   Expresses full-length Serotransferrin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TF (TF Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)

   Plasmid

   #73475

   Purpose

   Encodes the G protein to enable transsynaptic RAV infection

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   H2B-GFP-P2A-N2c(G)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 21, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pCB128

   Plasmid

   #205137

   Purpose

   Expression of mCherry-2xFYVE in Dictyostelium

   Depositor

   Jason King

   Article

   Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

   Insert

   2xFYVE (from human Hrs) (HGS Synthetic)
   

   Use

   Dictyostelium expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMOS012E: Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Plasmid

   #163074

   Purpose

   Entry vector for: Grx1-roGFP1-iE_ER glutathione redox sensor (endoplasmic reticulum)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   Grx1-roGFP1-iEER
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RabV CVS-N2c(deltaG)-hChR2-YFP

   Plasmid

   #73465

   Purpose

   Expresses channelrhodopsin fused to YFP for monosynaptic circuit mapping and optogenetic activation

   Depositor

   Thomas Jessell

   Article

   Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

   Insert

   hChR2-YFP
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

   Plasmid

   #159847

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNP1 Aqua toehold switch

   Plasmid

   #107356

   Purpose

   Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold switch 8 + Aquamarine
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pK RagA(Q66L)-RagC

    Plasmid

    #99700

    Purpose

    Bacterial expression of RagA(Q66L)-RagC

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag, Poly Arg, and SUMO

    Expression

    Bacterial

    Mutation

    L91P

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

    Plasmid

    #159846

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCFJ2211 - [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)

    Plasmid

    #159866

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(intron, PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

    Plasmid

    #182355

    Purpose

    mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ2089 - [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)

    Plasmid

    #159874

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - SNAP(PATC, no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET23A-Fucosidase-E1_10125-Ruminococcus_gnavus-His

    Plasmid

    #207665

    Purpose

    Expresses fucosidase E1_10125 from Ruminococcus gnavus in BL21 cells, purified using His-tag

    Depositor

    Robert de Vries

    Article

    Spruit et al bioRxiv 2023.12.15.571923

    Insert

    Fucosidase E1_10125
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    amino acids 34-584 were used

    Promoter

    T7 promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pV1464

    Plasmid

    #111440

    Purpose

    N. castellii Solo CRISPR vector, marked with URA3 and NatR

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-dMASS_1

    Plasmid

    #209764

    Purpose

    Expression of dMASS_1 in mammalian cells under CMV promoter

    Depositor

    Andre Berndt

    Article

    Rappleye et al ACS Sens. 2023 Nov 24;8(11):4233-4244. d

    Insert

    dMASS_1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV-CAG-FLEX-NEUROD1-T2A-mCherry

    Plasmid

    #178584

    Purpose

    Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    NEUROD1 (NEUROD1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJFRC210-10XUAS-FRT>STOP>FRT-myr::smGFP-OLLAS

    Plasmid

    #63170

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-OLLAS reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-OLLAS"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pETDuet RagA-RagC(S75N)

    Plasmid

    #99645

    Purpose

    Bacterial expression of RagA-RagC(S75N)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    S75N

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBabe_puro_DEST_Flag_SLC2A2

    Plasmid

    #45267

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SLC2A2 (SLC2A2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

23. pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

    Plasmid

    #182347

    Purpose

    GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCFJ2300 - [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159869

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-Dendra2(1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pWL502-crRNA

    Plasmid

    #174383

    Purpose

    The plasmids carrying a mini-CRISPR array were used to express crRNAs; article demonstrates use in Haloferax mediterranei

    Depositor

    Jing Han

    Article

    Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

    Insert

    a crRNA targeting the template strand
    

    Use

    CRISPR; Archaeal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PhaR promoter

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Lenti-sgKmt2d-sg3

    Plasmid

    #192167

    Purpose

    Lenti-sgKmt2d-sg3

    Depositor

    Sidi Chen

    Article

    Wang et al Cancer Discov. 2020 Dec;10(12):1912-1933

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEX 4t1 RARalpha (1-187)

    Plasmid

    #35558

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 1-187

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

28. RabV CVS-N2c(deltaG)-APEX2-Venus

    Plasmid

    #73467

    Purpose

    Expresses APEX2 fused to Venus for transsynaptic tracing and the EM analysis of infected neurons.

    Depositor

    Thomas Jessell

    Article

    Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

    Insert

    APEX2-Venus
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGEX 4T1 RARalpha (186-462)

    Plasmid

    #35559

    Depositor

    Jonathan Kurie, Harish Srinivas

    Article

    Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

    Insert

    RARalpha (RARA Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 186-462

    Promoter

    Available since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

30. pMTTH+eGFP-bromo5(N140A)

    Plasmid

    #157806

    Purpose

    Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1 with N140A mutation

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    eGFP-bromo5(N140A)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    N140A

    Promoter

    pTAC

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCW57.1-MBD3L2

    Plasmid

    #106332

    Purpose

    All-in-one lentivector with tet-inducible MBD3L2

    Depositor

    Stephen Tapscott

    Article

    Campbell et al Elife. 2018 Mar 13;7. pii: 31023. doi: 1

    Insert

    methyl-CpG binding domain protein 3 like 2 (MBD3L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCFJ2240 - [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159855

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T (1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. 1530_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-EmGFP-spA

    Plasmid

    #109317

    Purpose

    Plasmid for liver-specific expression of EmGFP with a BbsI cloning site for a gRNA

    Depositor

    William Lagor

    Article

    Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

    Plasmid

    #159873

    Purpose

    Three-fragment Gateway compatible protein tag for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCK051 p5E exorh:mCerulean

    Plasmid

    #195952

    Purpose

    Gateway p5E vector with reporter

    Depositor

    Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    exorh:mCerulean
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pSEM386 - mosTI unc-119 - NNN - gfp - tbb-2

    Plasmid

    #182345

    Purpose

    GFP fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcFLAG-Grap2-WT

    Plasmid

    #74511

    Purpose

    Overexpression of Grap2 in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Chougule et al Oncotarget. 2016 Feb 15. doi: 10.18632/o

    Insert

    Grap2 (GRAP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pCFJ2241 - [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)

    Plasmid

    #159860

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-mCardinal (1 intron, syntrons(3), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBabe_puro_DEST_Flag_YFP

    Plasmid

    #45278

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    YFP
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pBabe_puro_DEST_Flag_SAMD7

    Plasmid

    #45275

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SAMD7 (SAMD7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

41. pMDJ15 - pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]

    Plasmid

    #159801

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mCardinal (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSEM389 - mosTI unc-119 - NNN - mScarlet(nls) - tbb-2

    Plasmid

    #182349

    Purpose

    mScarlet transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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43. pK RagA-RagC

    Plasmid

    #99649

    Purpose

    Bacterial expression of wildtype Rag GTPase dimer

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    10x His tag, Poly arginine linker, and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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44. pCFJ2210 - [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)

    Plasmid

    #159864

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mMaple3(1 intron, syntrons(3), no_start, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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45. LjUBQ:MtGLV6:T35s

    Plasmid

    #164741

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr3g095180.1
    

    Use

    Tags

    Terminator 35S

    Expression

    Plant

    Mutation

    Promoter

    Lotus japonicus Ubiquitin

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. LjUBQ:MtGLV2:T35s

    Plasmid

    #164740

    Purpose

    Overexpression of Peptide coding gene (Binary vector)

    Depositor

    Wolf Scheible

    Article

    Roy et al Plant J. 2024 May;118(3):607-625. doi: 1

    Insert

    Medtr5g012400.1
    

    Use

    Tags

    Terminator 35S

    Expression

    Plant

    Mutation

    Promoter

    Lotus japonicus Ubiquitin

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBabe_puro_DEST_Flag_SLC7A14

    Plasmid

    #45261

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SLC7A14 (SLC7A14 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2013

    Availability

    Academic Institutions and Nonprofits only

48. pZ004 (LseC2c2 locus)

    Plasmid

    #70170

    Purpose

    Expresses LseC2c2 locus with one spacer in array

    Depositor

    Feng Zhang

    Article

    Shmakov et al Mol Cell. 2015 Nov 5;60(3):385-97. doi:

    Insert

    LseC2c2 locus with one spacer in array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

49. pCD5-ss-D/bovine/France/5920/2014-HEFwtED-GCN4-sfGFP-ST

    Plasmid

    #175017

    Purpose

    Expresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFP

    Depositor

    Robert de Vries

    Article

    Nemanichvili et al Viruses. 2021 Mar 31;13(4). pii: v130405

    Insert

    OK-HEFwtED
    

    Use

    Tags

    GCN4-TEV-sfGFP-TwinStrep

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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50. pETDuet RagA-RagC(L91P)

    Plasmid

    #99647

    Purpose

    Bacterial expression of RagA-RagC(L91P)

    Depositor

    David Sabatini, Kuang Shen

    Article

    Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

    Inserts

    RagA (RRAGA Human)
    RagC (RRAGC Human)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    L91P

    Promoter

    Available since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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