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pAL119-hCTLA4-Ig

Plasmid

#25100

Depositor

Maria Castro

Article

Guillot et al Blood. 2003 Apr 15. 101(8):3325-33.

Insert

ytotoxic T-murine Lymphocyte Antigen 4 (Ctla4 Mouse)

Use

Adenoviral

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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peCALWY-1

Plasmid

#22233

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-1 Zn(II) sensor (ATOX1 Human, GFP's)

Expression

Mammalian

Mutation

linker consists of 9 GGSGGS repeats

Available Since

Nov. 18, 2009

Availability

Academic Institutions and Nonprofits only
hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

Plasmid

#99278

Purpose

Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Article

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mVenus-IRES-mCherry
Puro

Use

Lentiviral

Tags

H2B, IRES, PGK, mCherry, and mVenus

Promoter

EF1 alpha-human and PGK-mouse

Available Since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_ATP1.0mut-IRES-mCherry-CAAX

Plasmid

#167583

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in mammalian cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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MAC_C_CH60

Plasmid

#111662

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CH60 (HSPD1 Human)

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLPCX-PECAMTS

Plasmid

#45850

Depositor

Martin Schwartz

Article

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

PECAM1 (PECAM1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDuet TDP-43 M337V

Plasmid

#27464

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Expression

Bacterial

Mutation

Methionine 337 mutated to Valine (M337V)

Available Since

Feb. 10, 2011

Availability

Academic Institutions and Nonprofits only
pGL4 mIL-17 0.6kb promoter

Plasmid

#20126

Depositor

Warren Strober

Article

Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

Insert

mIL-17 (Il17a Mouse)

Use

Luciferase

Available Since

Jan. 16, 2009

Availability

Academic Institutions and Nonprofits only
pMIG FLAG B56 gamma3

Plasmid

#13803

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B56 gamma 3 Flag (PPP2R5C Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
EF1a_ETV2_P2A_Hygro_Barcode

Plasmid

#120436

Purpose

Barcoded lentiviral vector to express ETV2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLXSN16E6

Plasmid

#52395

Purpose

The plasmid, pLXSN16E6, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 gene.

Depositor

Denise Galloway

Article

Halbert et al J Virol. 1991 Jan;65(1):473-8.

Insert

HPV 16 E6

Use

Retroviral

Available Since

May 27, 2014

Availability

Academic Institutions and Nonprofits only

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pH1R-mCherry-N1

Plasmid

#84325

Purpose

Histamine-1 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H1R (HRH1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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PDX1-2A-eGFP

Plasmid

#66964

Purpose

PDX1-eGFP mini-vector donor for integration of the eGFP reporter into the PDX1 locus

Depositor

Danwei Huangfu

Article

Zhu et al Stem Cell Reports. 2015 May 28. pii: S22

Insert

pancreatic and duodenal homeobox 1 (PDX1 Human)

Use

Unspecified

Tags

2A-eGFP

Available Since

July 24, 2015

Availability

Academic Institutions and Nonprofits only

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SCORE

Plasmid

#40232

Depositor

Wolfhard Almers

Article

An et al Science. 2004 Nov 5;306(5698):1042-6.

Insert

Snap25b (SNAP25 Human)

Tags

CFP and Venus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA 3.1 Flag-rat-PTP1B

Plasmid

#35988

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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TRE:GFP

Plasmid

#163036

Purpose

FLiCRE reporter gene for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

GFP

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TBG-Cyto-TurboID

Plasmid

#149414

Purpose

Hepatocyte-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

Depositor

Jonathan Long

Article

Wei et al Nat Chem Biol. 2021 Mar;17(3):326-334. d

Insert

Cyto-TurboID

Use

AAV

Tags

V5

Promoter

TBG

Available Since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGEP179_pX330K

Plasmid

#137882

Purpose

Entry vector for multi-gRNA cloning

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_humanTRAIL _IRES_mCherry_PGK_tBFP

Plasmid

#85432

Purpose

Lentiviral vector for Gal4 inducible human full-length TRAIL with mCherry reporter and constitutive tBFP expression

Depositor

Wendell Lim

Article

Roybal et al Cell. 2016 Oct 6;167(2):419-432.e16. doi

Insert

human TRAIL

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only
pU6T7

Plasmid

#71462

Purpose

Expresses gRNA from hybrid human U6/T7 promoter for both cellular expression and in vitro transcription from same promoter (2 Gs at initiation)

Depositor

Willie Mark

Article

Romanienko et al PLoS One. 2016 Feb 5;11(2):e0148362. doi

Type

Empty backbone

Use

CRISPR

Expression

Bacterial and Mammalian

Promoter

human U6/phage T7

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only

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