We narrowed to 30,517 results for: Ide

1. pLyGo-Bs-3

   Plasmid

   #163141

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in B. subtilis. Vector encoding the AprE signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLyGo-Bs-2

   Plasmid

   #163140

   Purpose

   pLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyQ signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PamyL-PamyQ-PcryIIIA-cryIIIAstab49

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLyGo-Ec-3

   Plasmid

   #163129

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Tol2-GgVCL/*TBM

   Plasmid

   #162790

   Purpose

   MUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

   Depositor

   Laura Lafon-Hughes

   Article

   et al PeerJ

   Insert

   Mutated Gallus gallus vinculin (G454V) (VCL Chicken)
   

   Use

   Tags

   IRES-GFP

   Expression

   Bacterial and Mammalian

   Mutation

   changed Gly 454 to Val

   Promoter

   beta actin

   Available since

   May 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLyGo-Ec-5

   Plasmid

   #163131

   Purpose

   pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDUAL_2_lacZ

   Plasmid

   #155173

   Purpose

   see pDUAL_lacZ: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDUAL_araBAD

   Plasmid

   #155172

   Purpose

   BaSP and CuCBP co-expression: araBAD promoter controls BaSP expression

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDUAL_2_tacI

   Plasmid

   #155174

   Purpose

   see pDUAL_tacI: exchanged origin of replication (pBR322) and introduced bom site

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDUAL_lacZ

   Plasmid

   #155169

   Purpose

   BaSP and CuCBP co-expression: lacZ promoter controls BaSP expression

   Depositor

   Bernd Nidetzky

   Article

   Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

   Inserts

   Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
   Cellobiose phosphorylase from Cellulomonas uda
   

   Use

   Synthetic Biology

   Tags

   His Tag and Strep - Tag II

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBICI_2_medium

    Plasmid

    #155167

    Purpose

    see pBICI_medium: exchanged origin of replication (pBR322) and introduced bom site

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBICI_strong

    Plasmid

    #155165

    Purpose

    BaSP and CuCBP co-expression: strong RBS controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBICI_medium

    Plasmid

    #155164

    Purpose

    BaSP and CuCBP co-expression: medium RBS controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBICI_weak

    Plasmid

    #155163

    Purpose

    BaSP and CuCBP co-expression: weak RBS controls BaSP expression

    Depositor

    Bernd Nidetzky

    Article

    Schwaiger et al Biotechnol J. 2020 Jul 15:e2000063. doi:

    Inserts

    Sucrose Phosphorylase from Bifidobacterium adolescentis (BAD_RS00415 Bifidobacterium adolescentis)
    Cellobiose phosphorylase from Cellulomonas uda
    

    Use

    Synthetic Biology

    Tags

    His Tag and Strep - Tag II

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. D70_Ld_210610

    Plasmid

    #135883

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    dihydrolipoamide acetyltransferase precursorlike protein
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. D6_Ld_100150

    Plasmid

    #135822

    Purpose

    Expression of Leishmania donovani ectodomain in mammalian cells

    Depositor

    Gavin Wright

    Article

    Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

    Insert

    nucleoside phosphatase, putative
    

    Use

    Tags

    biotinylation sequence and His tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTAMACHis6_PiuD

    Plasmid

    #128953

    Purpose

    expresses PiuD in E.coli

    Depositor

    James Naismith

    Article

    Luscher et al Antimicrob Agents Chemother. 2018 May 25

    Insert

    Putative outer membrane receptor for iron transport (PiuD)
    

    Use

    Tags

    6xHis and TamA

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed (1-105)

    Promoter

    T7

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-Xl-VSP1 R152Q

    Plasmid

    #51881

    Purpose

    expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

    Depositor

    Laurinda Jaffe

    Article

    Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

    Insert

    VSP1 R152Q (tpte2 Frog)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mammalian

    Mutation

    R152Q: near the extracellular side of the fourth …

    Promoter

    CMV

    Available since

    May 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLHCX-FLAG-mPKM2

    Plasmid

    #44239

    Depositor

    Lewis Cantley, Matthew Vander Heiden

    Article

    Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

    Insert

    Pyruvate Kinase M2 (Pkm Mouse)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pCAG-CFP-SaCas9-HF (without sgRNA)

    Plasmid

    #134470

    Purpose

    Human expression vector for SaCas9-HF variant.

    Depositor

    Zongli Zheng

    Article

    Tan et al Proc Natl Acad Sci U S A. 2019 Sep 30. p

    Insert

    Staphylococcus aureus Cas9 nuclease (SauCas9) with high genome-wide specificity
    

    Use

    CRISPR

    Tags

    SV40NLS-3xFLAG

    Expression

    Mammalian

    Mutation

    R245A, N413A, N419A, R654A

    Promoter

    CAG

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. modRNAc0-ABE8e-P2A-GFP

    Plasmid

    #178177

    Purpose

    IVT template plasmid for making ABE8e-P2A-GFP modRNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    ABE8e
    

    Use

    Ivt template plasmid

    Tags

    P2A-GFP

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge