Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 42,534 results for: Eras

Showing: 4101 - 4120 of 42534 results

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pTT3-SP-6xHis-NTRK1(FL)-FLAG

Plasmid

#157620

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NTRK1 (NTRK1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CEACAM5-COMP5AP-AviTag-9xHis

Plasmid

#157501

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM5 (CEACAM5 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-BirA*-KRASG12V

Plasmid

#122208

Purpose

Expresses myc-BirA* fused to KRASG12V in mammalian cells

Depositor

Christopher Counter

Article

Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

Insert

KRASG12V (KRAS Human)

Tags

myc-BirA*

Expression

Mammalian

Mutation

changed G12 to V

Promoter

CMV

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-KIR2DL1(FL)-FLAG

Plasmid

#157625

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL1 (KIR2DL1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-KIR2DL4(FL)-FLAG

Plasmid

#157624

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL4 (KIR2DL4 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pSB123 - pL0_rLUC-I (CDS1)

Plasmid

#123181

Purpose

Golden Gate (MoClo; CDS1) compatible luciferase gene from Renilla reniformis with an intron for transient and stable expression in plants

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants

Use

Luciferase; Part for plant expression

Expression

Plant

Mutation

No BpiI and BsaI sites; codon-optimised for plants

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CEACAM6-COMP5AP-AviTag-9xHis

Plasmid

#157287

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CEACAM6 (CEACAM6 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Izumo1-1D4

Plasmid

#183544

Purpose

Expression vector of mouse izumo sperm-egg fusion 1 (Izumo1) tagged with 1D4 at C-terminus.

Depositor

Masahito Ikawa

Article

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

izumo sperm-egg fusion 1 (Izumo1 Mouse)

Tags

1D4

Expression

Mammalian

Available Since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis

Plasmid

#157534

Purpose

Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3E (SEMA3E Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFSF14-COMP5AP-AviTag-9xHis

Plasmid

#157149

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFSF14 (TNFSF14 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NPHS1-COMP5AP-AviTag-9xHis

Plasmid

#157587

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NPHS1 (NPHS1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-LAIR1-COMP5AP-AviTag-9xHis

Plasmid

#157101

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

LAIR1 (LAIR1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-VSTM2L-Fc(DAPA)-AviTag-6xHis

Plasmid

#156582

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

VSTM2L (VSTM2L Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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155_KRAS in pmiRGlo

Plasmid

#78132

Purpose

luciferase reporter for miRNA activity

Depositor

Heidi Schwarzenbach

Article

Eichelser et al Oncotarget. 2014 Oct 30;5(20):9650-63.

Insert

KRAS (KRAS Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

3'UTR containing miRNA binding sites

Promoter

PGK

Available Since

May 16, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_884-1102 USP7

Plasmid

#131249

Purpose

Mammalian expression of the USP7 UBL4-5 domains with an N-terminal Myc tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Encodes USP7 UBL domains 4-5 (USP7 amino acids 88…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-TNFRSF12A-COMP5AP-AviTag-9xHis

Plasmid

#157052

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

TNFRSF12A (TNFRSF12A Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-NCR1-COMP5AP-AviTag-9xHis

Plasmid

#157253

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NCR1 (NCR1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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hTph2p-Luc

Plasmid

#223526

Purpose

Firefly-luciferase reporter expression driven by human Tph2 promoter

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Tph2 promoter (TPH2 Human)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

hTph2 promoter

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Psicheck Cdk2ap1Exon1

Plasmid

#178028

Purpose

expression vector to test translation efficiency by luciferase reporter.

Depositor

Lin He

Article

Modzelewski et al Cell. 2021 Oct 7. pii: S0092-8674(21)011

Insert

Cdk2ap1 (Cdk2ap1 Mouse)

Use

Luciferase; Nonviral

Tags

Luciferase

Expression

Mammalian

Promoter

Cloned in

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-ROBO1-COMP5AP-AviTag-9xHis

Plasmid

#157562

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

ROBO1 (ROBO1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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