Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 22,588 results for: his

Clear keywords
Showing: 4121 - 4140 of 22588 results

  1. BMR1_01g03280-bio-His

    Plasmid
    #107669
    Purpose
    Expresses enzymatically monobiotinylated full-length BMR1_01g03280 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.
    Insert
    BMR1_01g03280
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available Since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    107669-plasmid-map-sequence-id-211177

    Enlarge

  2. pET28-his-3C_Clu3B6p

    Plasmid
    #100093
    Purpose
    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide
    Depositor
    Nikolai Sluchanko
    Article
    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10
    Insert
    14-3-3 sigma human (SFN Human)
    Tags
    HSPB6 peptide and His6
    Expression
    Bacterial
    Mutation
    75-77 residues changed to AAA to facilitate cryst…
    Promoter
    T7
    Available Since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100093-plasmid-map-sequence-id-193872

    Enlarge

  3. His 3C MKK4 20-399

    Plasmid
    #87880
    Purpose
    E. coli expression of MKK4 aa 20-399
    Depositor
    Peter Mace
    Insert
    MKK4 (MAP2K4 Human)
    Tags
    3C protease site and 6xHis
    Expression
    Bacterial
    Mutation
    aa 20-399
    Promoter
    T7
    Available Since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87880-plasmid-map-sequence-id-174924

    Enlarge

  11. pBJG1 T7-8xHis-OGT D554N

    Plasmid
    #154279
    Purpose
    Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    Aspartate 554 changed to Asparagine- bacterial co…
    Available Since
    Sept. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    154279-plasmid-map-sequence-id-304834

    Enlarge

  12. pET-dHeFSpCas9-VP64-6xHis

    Plasmid
    #92119
    Purpose
    Expression of dead/inactive increased fidelity HeFSpCas9-VP64-6xHis in bacterial cells
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Insert
    dead/inactive HeFSpCas9-NLS-3xFLAG-VP64
    Use
    CRISPR
    Tags
    3xFLAG, 6xHis, NLS, and VP64
    Expression
    Bacterial
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    T7
    Available Since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92119-plasmid-map-sequence-id-194893

    Enlarge

  18. pSG73-β1ARts-sfGFP

    Plasmid
    #102459
    Purpose
    Expresses a fusion protein of thermostabilized β1 adrenergic receptor and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 104455
    Insert
    β1AR
    Tags
    His tag and sfGFP
    Expression
    Bacterial
    Promoter
    OR2-OR1-Pr
    Available Since
    Oct. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    102459-plasmid-map-sequence-id-200297

    Enlarge

  19. pTH861-gst-optec P210T

    Plasmid
    #89595
    Purpose
    Plasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase P210T mutant, codon-optimised for bacterial expression
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed proline 210 to threonine
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89595-plasmid-map-sequence-id-206078

    Enlarge

  20. pTH860-gst-optec A180Q

    Plasmid
    #89594
    Purpose
    Plasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codons
    Depositor
    Tobias von der Haar
    Article
    Jossé et al bioRxiv 200006
    Insert
    glutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
    Tags
    8xhistidine
    Expression
    Bacterial
    Mutation
    changed alanine 180 to glutamine
    Promoter
    T7
    Available Since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    89594-plasmid-map-sequence-id-206044

    Enlarge

Showing: 4121 - 4140 of 22588 results