We narrowed to 5,112 results for: mcs

1. pKM51

   Plasmid

   #154154

   Purpose

   Firefly luciferase under the control of the Drosophila melanogaster minimal Hsp70 promoter with 8 copies of the Aedes aegypti Rel1A NF-κB site

   Depositor

   Kevin Maringer

   Article

   Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

   Insert

   Firefly luciferase
   

   Use

   Luciferase

   Promoter

   Drosophila melanogaster minimal Hsp70 promoter wi…

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCKan-yibDp-SBS-mCherry

   Plasmid

   #134598

   Purpose

   Low Phosphate inducible SBS (bonryl-diphosphate) synthase with C-terminal mCherry)

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   SBS-mCherry
   

   Tags

   mCherry

   Expression

   Bacterial

   Promoter

   yibDp

   Available Since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-PTEN-3'UTR

   Plasmid

   #97204

   Purpose

   Expresses PTEN 3'UTR in mammalian cells

   Depositor

   Reproducibility Project Cancer Biology

   Article

   Kerwin et al Elife. 2020 Apr 21;9. pii: 51019. doi: 1

   Insert

   PTEN 3' UTR (PTEN Human)
   

   Tags

   none

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM-PAT-ProCDKA;1::CDKA;1:YFP

   Plasmid

   #80685

   Purpose

   binary Expression vector for plant expression

   Depositor

   Arp Schnittger, Nico Dissmeyer

   Article

   Nowack et al Nature. 2007 May 17;447(7142):312-5. Epu

   Insert

   CDKA;1 (CDC2 Mustard Weed)
   

   Tags

   YFP

   Expression

   Plant

   Promoter

   ProCDKA;1

   Available Since

   Aug. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. B0033m_BC

   Plasmid

   #66021

   Purpose

   MoClo Basic Part: RBS - Weiss RBS, low strength. Modified from Bba_B0033 to adjust spacing in MC system. [B:B0033m:C]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   RBS
   

   Use

   Synthetic Biology

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. B0034m_BC

   Plasmid

   #66022

   Purpose

   MoClo Basic Part: RBS - Weiss RBS, high strength. Modified from Bba_B0034 to adjust spacing in MC system. [B:B0034m:C]

   Depositor

   Douglas Densmore

   Article

   Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

   Insert

   RBS
   

   Use

   Synthetic Biology

   Available Since

   Jan. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nsp3 Mac1 - P43 (Oxford)

   Plasmid

   #169210

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Nsp3 Mac1 - C2 (UCSF)

   Plasmid

   #169209

   Purpose

   Expression of 6xHis tagged protein for crystallization, includes TEV protease site for tag removal

   Depositor

   James S Fraser

   Article

   Schuller et al Sci Adv. 2021 Apr 14;7(16). pii: 7/16/ea

   Insert

   SARS-CoV-2 Nsp3 Mac1
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. MSCV-N MCV ER

   Plasmid

   #37860

   Depositor

   Karl Munger

   Article

   Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

   Insert

   MCV ER (MCPyV_gp2, MCPyV_gp1 Human Merkel Cell Virus)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Promoter

   PKG

   Available Since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

10. AAV-syn-GCaMP6m-XC

    Plasmid

    #118975

    Purpose

    Improved genetic encoded calcium sensor based on GCaMP6m

    Depositor

    Xiaodong Liu

    Article

    Yang et al Nat Commun. 2018 Apr 17;9(1):1504. doi:

    Insert

    GCaMP6m-XC
    

    Use

    AAV

    Available Since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-GfaABC1D-lck-smV5-4x6T-WPRE

    Plasmid

    #196416

    Purpose

    Astrocytic expression of membrane-targeted V5 spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smV5
    

    Use

    AAV; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. DHB-mVenus

    Plasmid

    #136461

    Purpose

    Fluorescently tagged segment of DNA Helicase B that translocates from the nucleus to the cytoplasm in response to phosphorylation by CDK2

    Depositor

    Sabrina Spencer, Tobias Meyer

    Article

    Spencer et al Cell. 2013 Oct 10;155(2):369-83. doi: 10

    Insert

    DNA Helicase B (HELB Human)
    

    Use

    Lentiviral

    Tags

    mVenus

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK170.AAV-TRE-Cre-WPRE (Supernova)

    Plasmid

    #85040

    Purpose

    For AAV-TRE-Cre based Supernova, pK170 should be used with pK168 etc.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nlsCre-WPRE
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Nov. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPonA-BI-Gl NORM-LacZA TER- LacZB

    Plasmid

    #86212

    Purpose

    beta Globin NMD construct with and without PTC under Bi-directional Ponasterone A inducible promoter

    Depositor

    Robert Singer

    Article

    Trcek et al Genes Dev. 2013 Mar 1;27(5):541-51. doi:

    Insert

    beta-Globin (HBB, Hbb Human, Mouse)
    

    Use

    Ponasterone a inducible promoter

    Tags

    lacZA or lacZB

    Expression

    Mammalian

    Mutation

    39TER in beta Globin in MCS II

    Promoter

    Ponasterone A inducible

    Available Since

    Jan. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-SFFV-KTN1-WPRE-UbC-Emerald

    Plasmid

    #225941

    Purpose

    Lentiviral vector plasmid expressing human kinectin 1 (KNT1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Evan Reid, Bart Nieuwenhuis

    Inserts

    KTN1 (KTN1 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV2-SynTetOff-palGFP

    Plasmid

    #190232

    Purpose

    AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

    Depositor

    Hiroyuki Hioki

    Article

    Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

    Insert

    EGFP
    

    Use

    AAV

    Tags

    palmitoylation signal sequence

    Expression

    Mammalian

    Available Since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTSin EGFP

    Plasmid

    #127695

    Purpose

    Plasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

    Depositor

    Bryan Roth, Justin English

    Article

    English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Available Since

    July 3, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-GfaABC1D-lck-smMyc-4x6T-WPRE

    Plasmid

    #196415

    Purpose

    Astrocytic expression of membrane-targeted Myc spaghetti monster reporter in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    Lck-smMyc
    

    Use

    AAV; Astrocyte-selective

    Tags

    Lck

    Expression

    Mammalian

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH_Str-KDEL_ManII-SBP-EGFP

    Plasmid

    #65258

    Purpose

    synchronize trafficking of ManII from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Streptavidin-KDEL and truncated Mannosidase II
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available Since

    Aug. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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