LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
Plasmid
#67277
Purpose
inducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.
Depositor
Inserts
first casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
Tags
mTQ2 blue fluorescent and red flourescent gene ka…
Expression
Mammalian
Promoter
CAG
Available Since
Aug. 7, 2015
Availability
Academic Institutions and Nonprofits only