164,567 results
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Plasmid#196712PurposeCRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3DepositorInsertdCas9-KRAB(ZIM3)-T2A-TagBFP
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationD10A, N863APromoterEF1aAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRham-CALM1
Plasmid#182500PurposeBacterial expression of the Homo sapiens calmodulin, CALM1DepositorInsertCALM1 (CALM1 Human)
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-CAG-GFP (AAV8)
Viral Prep#37825-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-CAG-GFP (#37825). In addition to the viral particles, you will also receive purified pAAV-CAG-GFP plasmid DNA. CAG-driven GFP expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGria1
Plasmid#124853PurposeMutagenesis of Gria1DepositorInsertGria1 (Gria1 Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-OsTIR1(F74G)
Plasmid#140730PurposeOsTIR1(F74G)-P2A-mAID-EGFP-NESDepositorInsertOsTIR1(F74G)-P2A-mAID-EGFP-NES
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a_T7-ARG1
Plasmid#106473PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coliDepositorInsertARG1
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-EGFP
Plasmid#114213PurposeAAV vector (control) that targets expression of EGFP to neuronsDepositorInsertEnhanced Green Fluorescent Protein
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationNonePromoterhuman synapsin (hSyn)Available sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
eGFP-luciferase-C1
Plasmid#163523PurposeExpresses both eGFP and firefly luciferase in mammalian cellsDepositorInsertFirefly luciferase
UseLuciferaseTagseGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
MoClo Plant Parts II and Infrastructure Kit
Plasmid Kit#1000000135PurposeCompatible with the Modular Cloning (MoClo) system for hierarchical assembly of plant multigene constructs, with new phytobricks and vectors to connect Modular Cloning and Gateway Cloning platforms.DepositorAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK229
Plasmid#71256Purposeluciferase reposter with 229 bp of the HSV TK promoterDepositorInsertThymidine Kinase promoter
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterHSV Thymidine KinaseAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)- e18
Plasmid#131670PurposeExpresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.DepositorInserte18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124428PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Cas9-gRNA-GFP
Plasmid#124770Purpose3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backboneDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUCDM
Plasmid#110742PurposePlasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
MK01
Bacterial Strain#195090PurposeImproved E. coli strain created by knocking out the endogenous lacI gene with chloramphenicol acetyltransferase (cat) selection cassette in the parent strain BW27783 that overcome the all-or-none response of araBAD promoter (PBAD).DepositorBacterial ResistanceChloramphenicolAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLCNICK
Plasmid#84653PurposeGenome editing for Lactobacillus casei Lc2WDepositorInsertsCas9 nickase
sgRNA
homology arms of LC2W_2179
UseCRISPRTagsExpressionMutationD10APromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hM3D(Gq)-mCherry
Plasmid#50474PurposeGq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoterDepositorUseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterhuman Synapsin 1Available sinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-PARP1-Apex2–eGFP
Plasmid#211555PurposeA piggyBac vector containing CMV-PARP1-Apex2-eGFP-IRES-Neo cassette.DepositorInsertPARP1 (PARP1 Human)
UsePiggybacTagsApex2-eGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK1043
Plasmid#8835DepositorInserttobacco etch virus protease, catalytic domain
UseTagsMBP and polyarginineExpressionBacterialMutationAutolysis-resistant S219V mutantPromoterAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits only