Addgene

We narrowed to 38,776 results for: LAT;

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pnEA-NpM-HseIF4G_592-653_AG

Plasmid

#148746

Purpose

Bacterial Expression of HseIF4G_592-653

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G_592-653 (EIF4G1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-β-catenin_S23A,T40A,T41A,T112A

Plasmid

#190824

Purpose

For mammalian expression of β-catenin with 4 O-GlcNAc sites mutated (S23A, T40A, T41A and T112A) and GFP-tag on its N-terminus.

Depositor

Gerald Hart

Article

Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

Insert

β-catenin (CTNNB1 Chicken)

Use

Tags

GFP

Expression

Mammalian

Mutation

S23A, T40A, T41A, T112A

Promoter

CMV

Available since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676-GB1_AD

Plasmid

#148512

Purpose

Bacterial Expression of DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4Ga_617-630-DmThor_64-71-DmeIF4Ga_639-676 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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Coexpressed Free PcPTS

Plasmid

#182483

Purpose

Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Inserts

FPPS
PTS

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL10 and GAL7

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free NES (AG4TGGA)2

Plasmid

#182488

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Insert

FPPS-NES

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL10

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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Linked Free (PT)4P

Plasmid

#182490

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

Insert

FPPS-NES

Use

Synthetic Biology; Metabolic engineering

Tags

Expression

Yeast

Mutation

Promoter

GAL10

Available since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-1

Plasmid

#190095

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-1 (ABHD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

deleted the base pairs 6704-6709 refer to NM_0070…

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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ABHD2-3'UTR-Del-2

Plasmid

#190096

Purpose

examine the activity of ABHD2 3'UTR

Depositor

Bowen Liu

Article

Liu et al Commun Biol. 2022 Aug 13;5(1):816. doi:

Insert

ABHD2 3' UTR delete-2 (ABHD2 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

deleted the base pairs 7122-7129 refer to NM_0070…

Promoter

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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DDX11-1

Plasmid

#155503

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX11 (DDX11 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G1_615-654_AD

Plasmid

#148518

Purpose

Mammalian Expression of HseIF4G1_615-654

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654 (EIF4G1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G1_615-654-L640AV646A_AD

Plasmid

#148519

Purpose

Mammalian Expression of HseIF4G1_615-654-L640AV646A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-L640AV646A (EIF4G1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-LIVVtoAAAA_AD

Plasmid

#148523

Purpose

Mammalian Expression of HseIF4G1_615-654-LIVVtoAAAA

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-LIVVtoAAAA (EIF4G1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HseIF4G_615-654-Y619AL624AL625A_AD

Plasmid

#148524

Purpose

Mammalian Expression of HseIF4G1_615-654-Y619AL624AL625A

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

HseIF4G1_615-654-Y619AL624AL625A (EIF4G1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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CSTF3

Plasmid

#155487

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CSTF3 (CSTF3 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Mo-iPGM-bio

Plasmid

#179614

Purpose

Expresses M. orale iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

M. orale iPGM bio-6His

Use

Tags

His tag and biotinylation sequence

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Hp-iPGM-bio

Plasmid

#179615

Purpose

Expresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

H. pylori iPGM bio-6His

Use

Tags

His tag and biotinylation sequence

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pET21a Sa-iPGM-bio

Plasmid

#179523

Purpose

Expresses S. aureus iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

S. aureus iPGM bio-6His

Use

Tags

His tag and biotinylation sequence

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-chPKCdelta/eDHFR(69K6)-EGFP

Plasmid

#178857

Purpose

Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to EGFP

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-EGFP (PRKCD Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-chPKCdelta/eDHFR(69K6)-miRFP670

Plasmid

#178858

Purpose

Mammalian expression of PKCδ-eDHFR(69K6) chimera fused to miRFP670

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

PKCδ(1-229)-eDHFR(69K6)-PKCδ(280-675)-miRFP670 (PRKCD Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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