We narrowed to 13,747 results for: TIM;

1. pAAV-CaMKII-GAL4-iLight-VP16

   Plasmid

   #170274

   Purpose

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16 construct expression in mammalian cells. pAAV vector.

   Depositor

   Vladislav Verkhusha

   Article

   Kaberniuk et al Nat Commun. 2021 Jun 23;12(1):3859. doi:

   Insert

   NLS-Gal4(1-65)-iLight(human codon optimized)-VP16
   

   Use

   AAV

   Tags

   Expression

   Mutation

   See Comments

   Promoter

   CaMKII

   Available since

   June 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Designed VEGF-binding scFv G6des13

   Plasmid

   #110213

   Purpose

   Yeast Surface Display of G6des13

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des13
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95F

   Promoter

   Available since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Puro_siKO

   Plasmid

   #86696

   Purpose

   Tetracycline inducible expression of guide RNA targeted to AAVS1 locus

   Depositor

   Ludovic Vallier

   Article

   Bertero et al Development. 2016 Dec 1;143(23):4405-441

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   H1 TO

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. CRY2olig-mCh-CLC

   Plasmid

   #60035

   Purpose

   Expresses a fusion of CRY2olig (PHR, E490G) with mCherry and mouse clathrin light chain

   Depositor

   Chandra Tucker

   Article

   Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

   Insert

   CRY2-mCh-Clathrin Light Chain (CRY2 Mustard Weed, Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   M354I, E490G CRY2, A9D*

   Promoter

   CMV

   Available since

   Oct. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Designed VEGF-binding scFv G6des1

   Plasmid

   #110212

   Purpose

   Yeast Surface Display of G6des1

   Depositor

   Sarel Fleishman

   Article

   Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

   Insert

   G6des1
   

   Use

   Tags

   cmyc tag

   Expression

   Yeast

   Mutation

   L A43P, L Y49F, L Y55F ,L Q89L, L T94V, L P95T, L…

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJR107

   Plasmid

   #187245

   Purpose

   Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expression

   Depositor

   Marco Jost, Jonathan Weissman

   Article

   Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

   Insert

   Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-CD28

   Plasmid

   #223596

   Purpose

   Lentiviral expression of human CD28 in mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

   Insert

   CD28 (CD28 Human)
   

   Use

   Lentiviral; mammalian expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET20b_Pa-PiuA

   Plasmid

   #128955

   Purpose

   expresses Pseudomonas aeruginosa PiuA in E. coli

   Depositor

   James Naismith

   Article

   Moynie et al Antimicrob Agents Chemother. 2017 Mar 24

   Insert

   iron transport outer membrane receptor (PiuA) (PA4514 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   6xHis and pelB leader

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed

   Promoter

   T7

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. p420-3

   Plasmid

   #71257

   Purpose

   luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter

   Depositor

   Peter Cockerill

   Article

   Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

   Insert

   3 copies of the GM420 NFAT-AP-1 site (CSF2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   GM-CSF minimal

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pU6-neo-TET

    Plasmid

    #51286

    Purpose

    expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    U6 (RNU6-1 Human)
    

    Use

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Mutation

    Promoter

    U6 upstream enhancer sequence

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. p59sheLL

    Plasmid

    #40628

    Depositor

    Simon Beddows

    Article

    Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

    Inserts

    HPV59 L1
    HPV59 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression

    Promoter

    CMV and Polio IRES

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJR106

    Plasmid

    #187244

    Purpose

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expression

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRN3P_T3_ABE8e_IVT

    Plasmid

    #201676

    Purpose

    Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

    Insert

    ecTadA(8e)-nSpCas9
    

    Use

    CRISPR; Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. INCTbiosyn-pUB-HspINTphiC31

    Plasmid

    #127518

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase phiC31.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase phiC31 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-ABE6.3

    Plasmid

    #102916

    Purpose

    expresses ABE6.3 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE6.3
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. NEK7 gRNA (BRDN0001162196)

    Plasmid

    #77740

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human NEK7

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    NEK7 (NEK7 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR1a-tagRFP-KDEL

    Plasmid

    #114177

    Purpose

    Gateway entry clone containing tagRFP with a signal peptide and KDEL ER retention signal for expression of tagRFP in the ER

    Depositor

    Nicolas Demaurex

    Article

    Nunes-Hasler et al Nat Commun. 2017 Nov 24;8(1):1852. doi:

    Insert

    tagRFP-KDEL
    

    Use

    Gateway entry clone

    Tags

    KDEL retention signal and signal peptide

    Expression

    Mutation

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. p39sheLL

    Plasmid

    #40627

    Depositor

    Simon Beddows

    Article

    Draper et al Vaccine. 2011 Nov 3;29(47):8585-90. Epub

    Inserts

    HPV39 L1
    HPV39 L2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Codon optimized for mammalian expression

    Promoter

    CMV and Polio IRES

    Available since

    Oct. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

19. Lenti_PGK-Cas9-P2A-HygR

    Plasmid

    #164135

    Purpose

    Lentiviral construct for the expression of SpCas9 driven by PGK promoter in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

    Insert

    SpCas9 (cas9 Synthetic)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSW002-Pc-TorA(sp)-mTurquoise2

    Plasmid

    #111254

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter followed by E. coli TorA signal peptide in frame with mTurquoise2 (codon optimized for P. fluorescens); export to periplasm

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    TorA(sp)-mTurquoise2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mTurquoise2 is codon optimized for expression in …

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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