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pKK-mCerulean-TEV

Plasmid

#105773

Purpose

Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mCerulean-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pLuc-OptoJNKi

Plasmid

#89744

Purpose

Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Nkx2-5YRD Y-A

Plasmid

#98611

Purpose

Gateway entry vector for NKX2-5 YRD Y-A

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 YRD Y-A (Nkx2-5 Mouse)

Use

Entry vector

Mutation

Substitutions of 7 tyr into Ala residues.Y233A; Y…

Available Since

Aug. 10, 2017

Availability

Academic Institutions and Nonprofits only

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KC1G3_HUMAN_D0

Plasmid

#79685

Purpose

This plasmid encodes the kinase domain of KC1G3. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

KC1G3 (CSNK1G3 Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
csd6_13-330_W158A/pET28b(+) N-tag

Plasmid

#71358

Purpose

csd6, construct:13-330, pET28b(+) N-tag, mutation:W158A

Depositor

SeWon Suh

Article

Kim et al J Biol Chem. 2015 Oct 9;290(41):25103-17

Insert

HP0518 (HP0518 changed Tryptophan 158 to Alanine)

Tags

Histidine tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF4D03

Plasmid

#70969

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Taf12 (Taf12 Fly)

Use

Gateway entry vector

Available Since

Dec. 16, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_CUS1F03

Plasmid

#71071

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Su(var)205 (Su(var)205 Fly)

Use

Gateway entry vector

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4D-no stop

Plasmid

#67337

Purpose

hArl4D without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Gateway entry vector

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARF4-no stop

Plasmid

#67311

Purpose

hArf4 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Gateway entry vector

Expression

Bacterial

Mutation

V53A, L123P and M134I mutations were unintended P…

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pENTR1A DHFR.dn-cHSF1

Plasmid

#134726

Purpose

Gateway entry vector for constitutively active dominant-negative HSF1 fused to the DHFR destabilized domain

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

DHFR.dn-cHSF1 (HSF1 Human)

Use

Entry vector for gateway cloning

Tags

DHFR

Promoter

None

Availability

Academic Institutions and Nonprofits only

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pBSVSG221KOblast

Plasmid

#59733

Purpose

For replacement of VSG221 with a blasticidin resistence gene in the VSG221 expression site in T.brucei.

Depositor

Gloria Rudenko

Insert

VSG221 knockout construct

Use

For homologous recombination into vsg221 expressi…

Available Since

July 15, 2015

Availability

Academic Institutions and Nonprofits only

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pEH-1

Plasmid

#36270

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Insert

EH-1

Available Since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHE-6

Plasmid

#36279

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Insert

HE-6

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pHE-3

Plasmid

#36276

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Insert

HE-3

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pHE-2

Plasmid

#36275

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Insert

HE-2

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pEH-4

Plasmid

#36273

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Insert

EH-4

Available Since

July 24, 2012

Availability

Academic Institutions and Nonprofits only

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pHE-5

Plasmid

#36278

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Insert

HE-5

Available Since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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pHE-4

Plasmid

#36277

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Insert

HE-4

Available Since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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pEH-3

Plasmid

#36272

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Insert

EH-3

Available Since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

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pEH-2

Plasmid

#36271

Depositor

Christian Raetz

Article

Chung et al Biochemistry. 2010 May 18;49(19):4126-37

Insert

EH-2

Available Since

June 13, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge