We narrowed to 29,612 results for: PLE

1. Arp2_pLib

   Plasmid

   #173676

   Purpose

   Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

   Depositor

   Roberto Dominguez

   Article

   Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

   Insert

   Arp2 (ACTR2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXSIP-GPR35-FLAG-APEX

   Plasmid

   #204706

   Purpose

   expresses GPR35-FLAG-APEX in mammalian cells

   Depositor

   William Kaelin

   Article

   Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

   Insert

   G protein-coupled receptor 35 (GPR35 Human)
   

   Use

   Tags

   FLAG, APEX

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLiP-G2-RacE

   Plasmid

   #188966

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA: gttagacgctgattacatggactagg
   racE
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PTPN13_PDZ_1

   Plasmid

   #103919

   Purpose

   Protein expression and purification of PTN13 PDZ1 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PTPN13 (PTPN13 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PTPN13_PDZ_3

   Plasmid

   #103921

   Purpose

   Protein expression and purification of PTN13 PDZ3 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PTPN13 (PTPN13 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. PTPN13_PDZ_2

   Plasmid

   #103920

   Purpose

   Protein expression and purification of PTN13 PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PTPN13 (PTPN13 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PTPN13_PDZ_5

   Plasmid

   #103923

   Purpose

   Protein expression and purification of PTN13 PDZ5 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PTPN13 (PTPN13 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. LN-entry

   Plasmid

   #128012

   Purpose

   Vector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancer

   Depositor

   Julius Brennecke

   Article

   Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

   Insert

   LN-3xFlag
   

   Use

   Lamdan entry vector

   Tags

   LN-3xFlag

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. FKBP-YPet-RanGAP*

   Plasmid

   #175245

   Purpose

   Bacterial expression and purification, SUMOylation substrate that can be recruited to FRB with rapamycin, Ypet is a FRET acceptor for CyPet

   Depositor

   Michael Rosen

   Article

   Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

   Insert

   RANGAP1 (RANGAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Amino acid 562 F to A

   Promoter

   tac

   Available since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJN601

    Plasmid

    #59790

    Purpose

    zf1::yfp fusion with floxed unc-119(+) within an intron of yfp. For creating zf1::yfp knock-ins using unc-119 as a marker

    Depositor

    Jeremy Nance

    Article

    Armenti et al Development. 2014 Dec;141(23):4640-7. do

    Inserts

    ZF1 domain from pie-1
    N-terminal half of yfp with introns
    intron containing floxed unc-119 gene in reverse orientation
    C-terminal half of yfp with introns
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXW117As(RS-E11)

    Plasmid

    #123145

    Purpose

    Arsenic sensor with two-layered amplifier cascade. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J117, ParsR, PhrpLE, Pecf11

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPuroR-MCS-GAL4-MiniWhite

    Plasmid

    #165890

    Purpose

    Puromycin resistant GAL4 driver vector with Mini-w+ CDS eye marker. Enhancer grammar GB20 entry point for custom enhancers. Cut-and-paste cloning can be used. Purple-white bacteria colony screening.

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable Empty Enhancer Driver
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Venus-G protein Gamma 5

    Plasmid

    #42200

    Depositor

    Narasimhan Gautam

    Article

    Ajith Karunarathne et al Biochem Biophys Res Commun. 2012 May 11;

    Insert

    Gamma 5 (Gng5 Rat)
    

    Use

    Tags

    Venus(1-155)

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pSR06

    Plasmid

    #69152

    Purpose

    read-outloxN mCherry to GFP switch for integration on ttTi5605, Mos Chr II

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    eGFP
    

    Use

    Cre/Lox; Mossci

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0 and codon-optimzed index…

    Promoter

    rps-27

    Available since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. 8200 Bicistronic_GFP_ires_hygro

    Plasmid

    #64375

    Purpose

    This a retroviral expression plasmid expressing GFP along with hygro resistance gene

    Depositor

    Georgios Stathopoulos

    Article

    Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

    Insert

    EGFP
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJZC39

    Plasmid

    #62333

    Purpose

    sgRNA + 1x PP7 with mCherry for mammalian cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    sgRNA + 1x PP7
    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Targets Tet3G, sequence: GTACGTTCTCTATCACTGATA

    Promoter

    CMV and U6

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. Arp3_pLib

    Plasmid

    #173677

    Purpose

    Arp3 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

    Depositor

    Roberto Dominguez

    Article

    Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

    Insert

    Arp3 (ACTR3 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA4/TO-myc NES-mCE(K294A) NLS-Flag

    Plasmid

    #82469

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme, inactive form

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mRNA capping enzyme (Rngtt Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    changed Lysine 294 to Alanine

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4/TO-myc NES-mCE NLS-Flag

    Plasmid

    #82468

    Purpose

    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal flag tagged mRNA capping enzyme

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    Myc-NES-mCE-Flag (Rngtt HIV, Mouse)
    

    Use

    Tags

    Flag and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPK-351

    Plasmid

    #157921

    Purpose

    pcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBD

    Depositor

    Phillip Kyriakakis

    Article

    Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

    Inserts

    PIF3-MTAD
    IRES
    PhyB(1-621)-SV40NLS-Gal4DBD
    

    Use

    Synthetic Biology; Optogenetics

    Tags

    HA tag and SV40NLS

    Expression

    Mammalian

    Mutation

    pif3 1-523

    Promoter

    CMV

    Available since

    Sept. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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