We narrowed to 4,671 results for: puc19

1. pUC19::ZmUBI-RWT4Ca+C-Tail

   Plasmid

   #237514

   Purpose

   Protoplast expression

   Depositor

   Gitta Coaker

   Article

   Sung et al Nat Genet. 2025 Apr 14. doi: 10.1038/s41

   Insert

   Rwt4Ca+C-Tail
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Add C-terminal extension

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC19-U6-Bs3-sgRNA (BsaI)

   Plasmid

   #188280

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2019 Apr 23;5:23. doi: 10.1

   Insert

   Bs3-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   U6

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19 KGA HA5'-mKO2-3'

   Plasmid

   #110406

   Purpose

   Homology donor for glutaminase KGA isoform knock-in, mKO2

   Depositor

   Sandra Martha Gomes Dias

   Insert

   GLS glutaminase (GLS Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. puC19 preACT1(del6) short BP-3'SS

   Plasmid

   #111333

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   shorter branch to 3' splice site distance

   Promoter

   Available since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. puC19 preACT1(del6) AcAc

   Plasmid

   #111332

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   3' splice site AcAc

   Promoter

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pre-Ubc4 BrG pUC19

   Plasmid

   #111322

   Purpose

   for transcription to Ubc4 pre-mRNA

   Depositor

   Christine Guthrie

   Article

   Abelson et al Methods Enzymol. 2010;472:31-40. doi: 10

   Insert

   UBC4 (UBC4 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   intron has branch-site A to G mutation

   Promoter

   Available since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19 gene 60 thru gap

   Plasmid

   #49160

   Purpose

   Template for generating gene 60 mRNA truncated two codons after coding gap

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 60
   

   Use

   Tags

   Expression

   Mutation

   gene 60 mRNA truncated two codons after coding ga…

   Promoter

   T7

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC19 gene 60 to gap + Cys

   Plasmid

   #49159

   Purpose

   Template for generating gene 60 mRNA, sequence 5' of coding gap + Cys

   Depositor

   Nils Walter

   Article

   Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

   Insert

   Bacteriophage T4 gene 600
   

   Use

   Tags

   Expression

   Mutation

   Coding gap and downstream sequence have been dele…

   Promoter

   T7

   Available since

   Jan. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. puC19 preACT1(del6) A259C

   Plasmid

   #111329

   Purpose

   T7 transcription

   Depositor

   Christine Guthrie

   Article

   Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

   Insert

   ACT1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   branch site A259C

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. puC19 preACT1(del6) C256A

    Plasmid

    #111331

    Purpose

    T7 transcription

    Depositor

    Christine Guthrie

    Article

    Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

    Insert

    ACT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    branch site C256A

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC19 - T7 pro - IRES - EGFP

    Plasmid

    #138586

    Purpose

    Express EGFP with an upstream T7 promoter in pUC19 backbone

    Depositor

    Fei Chen

    Article

    Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid

    #228253

    Purpose

    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    U6

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC19_msfGFP-EWSR1-repair-tempate

    Plasmid

    #237682

    Purpose

    For knock-in msfGFP to EWSR1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EWSR1 (EWSR1 Human)
    

    Use

    CRISPR

    Tags

    msfGFP

    Expression

    Mutation

    Promoter

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC19-link-mCherry-FLAG:cat

    Plasmid

    #211831

    Purpose

    Plasmid template that encodes the fluorescent protein mCherry with a FLAG tag next to chloramphenicol resistence gene. This template was used to copy out mcherry-FLAG-cat for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mCherry-FLAG-cat
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19_msfGFP-SRRM2-repair-template

    Plasmid

    #238247

    Purpose

    For knock-in msfGFP to SRRM2 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    SRRM2
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-link-sfGFP-FLAG:Kan

    Plasmid

    #211833

    Purpose

    Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    sfGFP-FLAG-Kan
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

    Plasmid

    #171129

    Purpose

    LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

    Depositor

    Benjamin Kleinstiver

    Article

    Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

    Insert

    U6 promoter entry plasmid for LwaCas13a crRNA cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC19-link-mVenus-FLAG:Kan

    Plasmid

    #211830

    Purpose

    Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mVenus-FLAG-Kan
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NA

    Promoter

    Available since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC19.TRIM28.FKBP.knock-in.mRuby2

    Plasmid

    #185008

    Purpose

    For knocking in FKBP F36V P2A mRuby2

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    FKBP F36V P2A mRuby2
    

    Use

    Cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-URA3-3'SEC13-GFP

    Plasmid

    #25445

    Depositor

    Benjamin Glick

    Article

    Rossanese et al J Cell Biol. 1999 Apr 5. 145(1):69-81.

    Insert

    Sec13-Green Fluorescent Protein
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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