We narrowed to 31,808 results for: ide
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Plasmid#78741PurposeHuman expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertAsCpf1 crRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
IMS-HyPer2
Plasmid#60248PurposeGenetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.DepositorInsertIMS-HyPer2
TagsMitochondrial intermembrane space targeting signa…ExpressionMammalianPromoterCMVAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-GFP-HiBit
Plasmid#170989PurposeFor bacterial expression of bdSUMO tagged muGFP with NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458_3XHA_dFnCas9_KRAB_T2A_eGFP
Plasmid#200389Purpose3XHA tagged dead Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expressionDepositorInsertPX458_3XHA_dFnCas9_KRAB_eGFP
UseCRISPRTags3xHA-NLS and SV40NLS-KRAB-T2A-EGFPExpressionMammalianPromoterCBHAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA-XMAS-1x
Plasmid#164413PurposeDual targeted guide and cloning vector. Targets pEF-XMAS-1xStop. Guides targeting endogenous sites can be cloned into BbsI sites.DepositorInsertsgRNA
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterU6Available SinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLHCX-gpASNase1
Plasmid#121526PurposeExpresses gpASNase1 in mammalian cells.DepositorInsertgpASNase1
UseRetroviralTagsFLAGExpressionBacterial and MammalianPromoterCMVAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-TRAC-miniGag-Cas9
Plasmid#228959PurposeminiEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag-Cas9
UseCRISPRExpressionMammalianAvailable SinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry
Plasmid#78534PurposeBackbone to clone single or paired sgRNAsDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterU6 (for expressing sgRNA)Available SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAD-SPS6-Pman
Plasmid#133906PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPmanAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
p11_AaC60αβ/C20
Plasmid#229506PurposeExpresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20DepositorInsertsChaperonin 60α
Chaperonin 60β
Chaperonin 20
ExpressionBacterialMutationN terminus chloroplast transit peptide truncationPromoterT7Available SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLEX GFP-HA
Plasmid#229501PurposeControl HA-tagged protein for immunoprecipitation experiments.DepositorInserteGFP
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable SinceFeb. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-mFlagEts1
Plasmid#86099Purposeexpresses flag tagged Ets1 in mammalian cellsDepositorAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-Cas9-2A-GFP
Plasmid#170181PurposeIVT template plasmid for making modified Cas9GFP RNADepositorInsertCas9-2A-GFP
UseIvt template plasmidPromoterT7 promoterAvailable SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pY026
Plasmid#84741PurposeExpresses huAsCpf1 and crRNA guideDepositorInserthuAsCpf1
Tags3xHA and NLSExpressionMammalianPromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEJS2424_Nme2.Smu-ABE8e-i8
Plasmid#201522PurposeMammalian expression of domain inlaid Nme2.Smu-ABE8e-i8DepositorInsertNme2.Smu-ABE8e-i8
ExpressionMammalianPromoterCMVAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherry_sgRNA-ver2
Plasmid#126776PurposegRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNADepositorInsertsgRNA (for SpCas9)
UseCRISPRExpressionMammalianPromoterU6Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor lambda version
Plasmid#66073PurposeThe burden monitor was cloned inside plasmid pAH63 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellsDepositorInsertGFP
Available SinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
hGip-R
Plasmid#14942DepositorAvailable SinceJune 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEJS2423_Nme2.Smu-ABE8e-i7
Plasmid#201521PurposeMammalian expression of domain inlaid Nme2.Smu-ABE8e-i7DepositorInsertNme2.Smu-ABE8e-i7
ExpressionMammalianPromoterCMVAvailable SinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only