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pGAD-hRubicon

Plasmid

#64144

Purpose

For yeast two hybrid

Depositor

Tamotsu Yoshimori

Article

Tabata et al Mol Biol Cell. 2010 Dec;21(23):4162-72.

Insert

Rubicon (RUBCN Human)

Tags

GAL4 activation domain

Expression

Yeast

Promoter

ADH1

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
tdminiSOG-Tubulin-6

Plasmid

#57808

Purpose

Localization: Microtubules, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Tubulin (TUBA1B Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pET28-p53 (1-73) (K24N)

Plasmid

#62079

Purpose

Expression of human p53 (1-73) (K24N) in e. coli

Depositor

Gary Daughdrill

Article

Zhan et al Proteins. 2013 Oct;81(10):1738-47. doi:

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

contains TAD residues 1-73; K24N

Promoter

T7

Available Since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
tdEos-Actin-7

Plasmid

#57570

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Betzig et al Science. 2006 Sep 15;313(5793):1642-5. E

Insert

Actin (ACTB Human)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mPapaya-Vimentin-7

Plasmid

#56646

Purpose

Localization: Intermediate Filaments, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Vimentin (VIM Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a S620T

Plasmid

#53059

Purpose

Expresses alpha subunit of S620T hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Casis et al Mol Pharmacol. 2006 Feb;69(2):658-65. Ep

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Serine 620 to Threonine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pSP64-hERG1a S631A

Plasmid

#53058

Purpose

Expresses alpha subunit of S631A hERG1a K channel in Xenopus oocytes

Depositor

Michael Sanguinetti

Article

Zou et al J Physiol. 1998 May 15;509 ( Pt 1):129-3

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Use

Xenopus oocyte expression

Mutation

changed Serine 631 to Alanine

Promoter

SP6

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pX41

Plasmid

#46851

Purpose

TOP Hupki-wt plasmid without selection marker

Depositor

Monica Hollstein

Article

Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

Insert

attB and Hupki p53 wild type (TP53 Mouse, Human)

Expression

Mammalian

Available Since

Aug. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pM-ErbB4-delta-kinase-PY1 mutant

Plasmid

#17801

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
Protrudin-WT

Plasmid

#253736

Purpose

Control / baseline protrudin function

Depositor

Evan Reid

Article

Kleniuk et al Neurobiol Dis. 2026 Jan;218:107231. doi:

Insert

WT (TP53 Human)

Use

Retroviral

Expression

Mammalian

Mutation

WT

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-SOX9

Plasmid

#141404

Purpose

Inducible Expression of SOX9

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

SOX9 (SOX9 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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ATG9A-mCherry

Plasmid

#197393

Purpose

Expresses ATG9A in mammalian cells

Depositor

Juan Bonifacino

Article

De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

Insert

ATG9A (ATG9A Human)

Tags

mCh

Expression

Mammalian

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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DRD1-DuET

Plasmid

#213227

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

DRD1 (DRD1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pLIX403_control_APOBEC_HA_P2A_mRuby_Capture1

Plasmid

#183951

Purpose

Inducible lentiviral expression, TRE-control-APOBEC-HA-P2A-mRuby; PGK-puro-2A-rtTA

Depositor

Eugene Yeo

Article

Brannan et al Nat Methods. 2021 May;18(5):507-519. doi

Insert

APOBEC1 (Apobec1 Rat)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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FKBP:FRB-FBAR

Plasmid

#223620

Purpose

Transient expression of human FBP17 with mGFP, FRB, FKBP and TagBFP tags in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

FBP17 (FNBP1 Human)

Tags

FKBP, TagBFP and mGFP, FRB

Expression

Mammalian

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-ALFA-CD28

Plasmid

#223630

Purpose

Lentiviral expression of human CD28 with ALFA tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD28 (CD28 Human)

Use

Lentiviral; mammalian expression

Tags

ALFA tag

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-Gal3

Plasmid

#85662

Purpose

Fluorescent reporter for lysosomal damage

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

LGALS3 (LGALS3 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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ADRA1A-Tango

Plasmid

#66213

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ADRA1A (ADRA1A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pLS13-IL-10

Plasmid

#209128

Purpose

Transient mammalian expression of IL-10

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

IL-10 (IL10 Human)

Expression

Mammalian

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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FRB-mCherry-FTH1

Plasmid

#100749

Purpose

Part of FerriTag expression system. Encodes human Ferritin with an FRB domain and mCherry tag

Depositor

Stephen Royle

Article

Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

Insert

ferritin heavy chain 1 (FTH1 Human)

Tags

FRB and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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