We narrowed to 7,024 results for: 11

1. pHR-Hsp-eGFP-PGK-mCherry

   Plasmid

   #191862

   Purpose

   Heat inducible eGFP reporter with constitutive mCherry marker

   Depositor

   Yingxiao Wang

   Article

   Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

   Inserts

   eGFP
   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   HSPA7 promoter and PGK promoter

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hsyn-GRAB_rDA1h

   Plasmid

   #140557

   Purpose

   Expresses the red fluorescent high-affinity DA sensor GRAB_rDA1h in neurons

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Has Service

   AAV9

   Insert

   Red fluorescent DA sensor GRAB_rDA1h (high affinity)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-EMTB-TurboID-V5-puro

   Plasmid

   #190741

   Purpose

   A lentiviral plasmid encoding EMTB fusion to V5-tagged TurboID on microtubules (EMTB as the microtubule-targeting tag)

   Depositor

   Jeremy Baskin

   Article

   Cao et al ACS Chem Biol. 2022 Aug 11. doi: 10.1021

   Insert

   EMTB-TurboID-V5 (RPS14 EMTB is human ensconsin; TurboID is engineered BirA from E.coli, Human)
   

   Use

   Lentiviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Microtubule binding-domain of ensconsin (amino ac…

   Promoter

   CMV

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNCST-AausFP1

   Plasmid

   #129499

   Purpose

   Expresses AausFP1 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AausFP1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS_4xEcoLeuT(CUA)_AnapRS

   Plasmid

   #140019

   Purpose

   Plasmid with 4xEcoLeuT(CUA) cassette and E. coli AnapRS for amber suppression and incorporation of the fluorescent ncAA Anap; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   EcoLeuRS (AnapRS)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

   Promoter

   EF1

   Available since

   May 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFPN1-gamma-tubulin

   Plasmid

   #87956

   Purpose

   Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The protein is a sh-gamma-tubulin resistant gene …

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET41b+_Ufd1-His

   Plasmid

   #117107

   Purpose

   Production of recombinant Ufd1-His in E.coli

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   Ufd1 (UFD1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET41b+_Npl4

   Plasmid

   #117108

   Purpose

   Production of recombinant Npl4 in E.coli

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   Npl4 (NPLOC4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-myc-EphB6

   Plasmid

   #200984

   Purpose

   Mammalian expression plasmid for myc-tagged EphB6

   Depositor

   Erik Procko

   Article

   Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

   Insert

   EphB6 (EPHB6 Human)
   

   Use

   Tags

   Extracellular c-myc epitope tag and Signal peptid…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIA33

    Plasmid

    #120803

    Purpose

    E. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfp

    Depositor

    David S. Weiss, Craig Ellermeier

    Article

    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

    Inserts

    deactivated nuclease dCas9, codon-optimized for C. difficile
    single guide RNA targeting red fluorescent protein
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Base-pairing region targets red fluorescent prote…

    Promoter

    Pgdh and Pxyl

    Available since

    Feb. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEEF1D-FLAG (pcDNA 3.1+) LG6-1

    Plasmid

    #37365

    Depositor

    David Litchfield

    Article

    Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

    Insert

    EEF1D (EEF1D Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pMSCVpuro-Flag-cMyc T58A

    Plasmid

    #20076

    Depositor

    Juan Belmonte

    Article

    Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

    Insert

    cMyc (MYC Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T58A

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 mHIF-2α MYC (P405A/P530V/N851A)

    Plasmid

    #44027

    Depositor

    Celeste Simon

    Article

    Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

    Insert

    mHIF-2α (P405A/P530V/N851A) (Epas1 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P405A, P530V, N851A

    Promoter

    CMV

    Available since

    March 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pQFlag-USP7 CS puroR

    Plasmid

    #46752

    Purpose

    Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

    Depositor

    Gordon Peters, Goedele Maertens

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP7 (USP7 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    C223S--catalytically inactive

    Promoter

    CMV

    Available since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIRES-hrGFP II-TET1_CD

    Plasmid

    #83570

    Purpose

    Expresses the catalytic domain of TET1 in mammalian cells

    Depositor

    Jean-Pierre Issa

    Article

    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

    Insert

    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    

    Use

    Tags

    3X FLAG tag and hr GFP II

    Expression

    Mammalian

    Mutation

    TET1 amino acids 1-1417 deleted

    Promoter

    CMV

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. CDK4-3XFlag-pBabe

    Plasmid

    #73558

    Purpose

    vector encoding a fusion protein between CDK4 and 3 Flag tags

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFPV25-cR-2iG

    Plasmid

    #204618

    Purpose

    Fluorescent reporter for SPI2 induction in Salmonella

    Depositor

    Jacques Neefjes

    Article

    Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

    Inserts

    rpsM promoter
    sseA promoter
    

    Use

    Tags

    GFP and dsRed

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. CMV-FLAG-TAOK2

    Plasmid

    #197862

    Purpose

    Expression of FLAG-tagged TAOK2 / PSK1 alpha in mammalian cells

    Depositor

    Jonathan Morris

    Article

    Koo et al Mol Cancer Ther. 2017 Nov;16(11):2410-24

    Insert

    TAOK2 (TAOK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Wild Type

    Promoter

    SV40

    Available since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1.Gαi3-LgB91

    Plasmid

    #134359

    Purpose

    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi3-LgB91 (GNAI3 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCEP4-myc-EphB2

    Plasmid

    #200982

    Purpose

    Mammalian expression plasmid for myc-tagged EphB2

    Depositor

    Erik Procko

    Article

    Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

    Insert

    EphB2 (EPHB2 Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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