We narrowed to 48,864 results for: des.1

1. pVH333-1-Tier1-PhCMV-dCas9-3xNLS-VP64

   Plasmid

   #169597

   Purpose

   Tier-1 vector encoding PhCMV-driven dCas9-3xNLS-VP64 expression (PhCMV-dCas9-3xNLS-VP64-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   dead S.pyogenes Cas9 - VP64 fusion
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-Ef1a-Cas9-GFP (gRNA: Ascl1-1)

   Plasmid

   #159656

   Purpose

   CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Ascl1.

   Depositor

   Joseph Brzezinski

   Article

   Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Ef1a

   Available since

   March 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6-sgRNA EF1Alpha-puro-T2A-BFP.DIS3L2-1

   Plasmid

   #128762

   Purpose

   Expresses DIS3L2 gRNA for CRISPRi

   Depositor

   David Bartel

   Article

   Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

   Insert

   DIS3L2 sgRNA #1 (DIS3L2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-1-Shp2 T7DA/S189D SH2s

   Plasmid

   #67577

   Purpose

   Expresses GST-tagged Shp2 T73D/S189D SH2 domains in bacteria

   Depositor

   Graeme Carnegie

   Article

   Burmeister et al J Biol Chem. 2015 May 8;290(19):12058-67

   Insert

   Src homology 2 domain-containing phosphatase 2
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Deleted amino acid residues 219-597, changed T73 …

   Promoter

   Available since

   Sept. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1/CIP2A(1-905) WT V5 His

   Plasmid

   #119287

   Purpose

   CIP2A WT V5 overexpression

   Depositor

   Jukka Westermarck

   Article

   Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

   Insert

   KIAA1524 (CIP2A Human)
   

   Use

   Tags

   V5 His

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-EF1a-LAMP-1-mGFP-IRES-Puromycin

   Plasmid

   #134868

   Purpose

   Expresses EGFP-tagged lysosomal marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Lysosomal-associated membrane protein 1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETDuet-1 Hepatitis C Virus NS3/NS4A

   Plasmid

   #61696

   Purpose

   inducible dual expression of NS3 protease along with its cofactor NS4A

   Depositor

   Michael Johnson

   Article

   Lee et al PLoS One. 2013 Oct 9;8(10):e75144. doi:

   Inserts

   NS4A
   NS3
   

   Use

   Tags

   his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmIL-6 mut AP-1+C/EBP

   Plasmid

   #61292

   Purpose

   drives luciferase from mouse IL-6 promoter with mutations in both AP-1 & C/EBP sites

   Depositor

   Gail Bishop

   Article

   Baccam et al J Immunol. 2003 Mar 15;170(6):3099-108.

   Insert

   IL-6 promoter (Il6 Mouse)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Mutated both AP-1 binding site from TGAGTCT to TG…

   Promoter

   IL-6 promoter with mutant AP-1 and C/EBP binding …

   Available since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

9. KIF1A(1-393)-GCN4-2xmCherry-EF(C)

   Plasmid

   #61665

   Purpose

   Tandem mCherry-labeled, forced-dimeric, truncated kinesin-3 fused to EF Hand linker

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   KIF1A(1-393)-GCN4-2xmCherry-EF(C) (KIF1A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PD-1-miSFIT-6-C, 16-C

    Plasmid

    #124681

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRK5 FLAG wildtype, catalytic dead lipin 1

    Plasmid

    #32006

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    Lipin1 (Lpin1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    D712E and D714E

    Promoter

    CMV, SP6

    Available since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pOPINK-Cezanne2 (OTU+UBA, aa 1-462)

    Plasmid

    #61582

    Purpose

    Expresses human Cezanne2 (OTU+UBA domains) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    Cezanne2 (OTUD7A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Isoform 2. Deleted aa 463-933.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. PD-1-miSFIT-12-C, 21-G

    Plasmid

    #124683

    Purpose

    Tuning expression of PD-1

    Depositor

    Tudor Fulga

    Article

    Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

    Insert

    PD-1 (PDCD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-puro U6 sgRNA Oct4A -158

    Plasmid

    #50922

    Purpose

    U6 driven sgRNA targeting OCT4 isoform A -158 bp from TSS

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Oct4A -158 sgRNA (POU5F1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLKO.1-puro U6 sgRNA Oct4A -12

    Plasmid

    #50921

    Purpose

    U6 driven sgRNA targeting OCT4 isoform A -12 bp from TSS

    Depositor

    Scot Wolfe, Rene Maehr

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Oct4A -12 sgRNA (POU5F1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3-HA-HA-human CMYC (1-170)

    Plasmid

    #203426

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3-HA-HA-human CMYC (1-208)

    Plasmid

    #203427

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3-HA-HA-human CMYC (1-277)

    Plasmid

    #203428

    Purpose

    Mammalian expression of MYC

    Depositor

    Yoshiaki Ito

    Article

    Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

    Insert

    MYC (MYC Human)
    

    Use

    Tags

    2 x HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3xFLAG-CMV-RNF8-N-terminus(1-140)

    Plasmid

    #64675

    Purpose

    expresses only N terminus of RNF8, amino acids 1 to 140

    Depositor

    Marianne Farnebo

    Article

    Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

    Insert

    Ring finger protein 8 (RNF8 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    only N terminus amino acids 1-140

    Promoter

    CMV

    Available since

    May 20, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

    Plasmid

    #64207

    Purpose

    mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 (CRY2 Mustard Weed)
    RGS4(del 1-33) (RGS4 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only