We narrowed to 5,112 results for: mcs

1. pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

   Plasmid

   #138989

   Purpose

   IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRA (Tcra Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV sMTase (eGFP)

   Plasmid

   #89930

   Purpose

   Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC-IRES-MN
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   deactivated Cas9

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA

   Plasmid

   #89873

   Purpose

   expresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV production

   Depositor

   Hyungbae Kwon

   Article

   Lee et al Nat Methods. 2017 May;14(5):495-503. doi

   Insert

   bArrestin2-TevC-P2A-TdTomato
   

   Use

   AAV

   Tags

   TdTomato

   Expression

   Mammalian

   Mutation

   R173H in TdTomato (Please see depositor comments …

   Promoter

   Human synapsin

   Available Since

   May 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-BDNF-Myc

   Plasmid

   #124279

   Purpose

   Express Myc-tagged brain-derived neurotrophic factor (BDNF)

   Depositor

   Baoji Xu

   Article

   Xie et al Cell Metab. 2019 Jan 11. pii: S1550-4131

   Insert

   BDNF (Bdnf Mouse)
   

   Use

   AAV

   Tags

   Myc

   Available Since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKIIa-mScarlet

   Plasmid

   #131000

   Purpose

   AAV vector to drive the expression of mScarlet under the control of CamKIIa promoter

   Depositor

   Karl Deisseroth

   Article

   Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

   Has Service

   AAV8

   Insert

   mScarlet
   

   Use

   AAV

   Promoter

   CaMKIIa

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH_Str-KDEL_ST-SBP-EGFP

   Plasmid

   #65267

   Purpose

   synchronize trafficking of ST from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Str-KDEL and truncated sialyltransferase (ST6GAL1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDH_CMV_dimer_mKate2

   Plasmid

   #167333

   Purpose

   Expresses mKate2 fused to TFAM no-dimerization mutant in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Changed K95A, Y99F, E106A, E112A and R116A

   Promoter

   CMV

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDH_CMV_HMGA_mKate2

   Plasmid

   #167334

   Purpose

   Expresses mKate2 fused to TFAM HMGA mutant in mammalian cells

   Depositor

   Tom Misteli

   Article

   Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

   Insert

   TFAM (TFAM Human)
   

   Use

   Lentiviral

   Tags

   mKate2

   Expression

   Mammalian

   Mutation

   Truncation mutant containing MTS (1-42) and HMGA …

   Promoter

   CMV

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV-SFFV-CKAP4 S3E S17E S19E-WPRE-UbC-Emerald

   Plasmid

   #225947

   Purpose

   Lentiviral vector plasmid expressing human CKAP4 mutations S3E S17E S19E under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

   Depositor

   Richard Eva, Bart Nieuwenhuis, Evan Reid

   Inserts

   CKAP4 (CKAP4 Human)
   Emerald
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   S3E S17E S19E

   Promoter

   SFFV and UbC

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-SFFV-CKAP4 S3A S17A S19A-WPRE-UbC-Emerald

    Plasmid

    #225948

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutations S3A S17A S19A under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis, Evan Reid

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    S3A S17A S19A

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH_TNF-SBP-EGFP

    Plasmid

    #65282

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Lentiviral

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Promoter

    CMV

    Available Since

    June 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

    Plasmid

    #197885

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPReporter-mCherry

    Plasmid

    #65008

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    User-defined

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV3Tag8li-hDDX50-HA

    Plasmid

    #165465

    Purpose

    Human DDX50 mammalian expression vector with C-terminal HA tag.

    Depositor

    Martin Dorf

    Article

    Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

    Insert

    DExD-box helicase 50 (DDX50 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-SFFV-myc-RRBP1-WPRE-UbC-Emerald

    Plasmid

    #225942

    Purpose

    Lentiviral vector plasmid expressing human ribosome binding protein 1 (RRBP1) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Richard Eva, Bart Nieuwenhuis, Evan Reid

    Inserts

    RRBP1 (RRBP1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Promoter

    SFFV and UbC

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-EF1α-Flex/3'USS-mCherry(ATG full mut)

    Plasmid

    #197893

    Purpose

    Can be used to generate AAV virus that will express mCherry in the presence of Cre and the leakage expression is significantly reduced without Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    mCherry
    

    Use

    AAV

    Mutation

    N/A

    Promoter

    EF1a

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE

    Plasmid

    #168106

    Purpose

    PiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloning

    Depositor

    Erich Jarvis

    Article

    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

    Insert

    mCherry-KASH
    

    Use

    CRISPR and Cre/Lox

    Promoter

    CAG

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOP/SSAT

    Plasmid

    #140291

    Purpose

    Expresses human SSAT in bacterial cells

    Depositor

    Saverio Bettuzzi, Gaetano Marverti

    Article

    Marverti et al Carcinogenesis. 2005 Oct;26(10):1677-86.

    Insert

    SSAT1 (SAT1 Human)
    

    Expression

    Bacterial

    Available Since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH_TNF-SBP-mCherry

    Plasmid

    #65283

    Purpose

    TNF reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    TNF (TNF Human)
    

    Use

    Lentiviral

    Tags

    Streptavidin Binding Protein (SBP) and mCherry

    Promoter

    CMV

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CMV-NFIB-T2A-miRFP670

    Plasmid

    #187222

    Purpose

    Expresses human NFIB and miRFP670 via T2A linker under control of a CMV promoter.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    Nuclear Factor I B (NFIB Human)
    

    Tags

    Inserted T2A-miRFP670 at 3' terminal of MCS.

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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