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We narrowed to 10,278 results for: CAD;

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Showing: 6331 - 6360 of 10278 results

  1. pCJ191

    Plasmid
    #162668
    Purpose
    pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 20 residue Gly/Ser linker
    Tags
    His
    Expression
    Bacterial
    Mutation
    codon optimized for expression in E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162668-plasmid-map-sequence-id-321523

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  7. pLenti PGK mKate2-OGT D554N (Puro)

    Plasmid
    #154292
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, D554N variant, no Ser/Thr glycosylation)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    D554N mutation (Ser/Thr glycosylation-incompetent)
    Promoter
    PGK
    Available Since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    154292-plasmid-map-sequence-id-332434

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  8. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid
    #58492
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    IRE1a (ERN1 Human)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    deleted amino acids P29 to D408 and bearing mutat…
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58492-plasmid-map-sequence-id-90361

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  11. pSicoR Dnmt1

    Plasmid
    #12166
    Purpose
    Cre-regulated lentiviral shRNA vector. Cre addition causes both EGFP and shRNA to be recombined out of the construct, turning OFF Dnmt1 shRNA expression.
    Depositor
    Tyler Jacks
    Article
    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1
    Insert
    Dnmt1 shRNA (Dnmt1 Mouse)
    Use
    Cre/Lox, Lentiviral, and RNAi
    Expression
    Mammalian
    Mutation
    EGFP is expressed from this plasmid as a marker, …
    Available Since
    June 30, 2006
    Availability
    Academic Institutions and Nonprofits only

  13. pD649-HAsp-NOTCH3(Trunc1)-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #157045
    Purpose
    Mammalian expression of cell-surface protein partial extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    NOTCH3 (NOTCH3 Human)
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    157045-plasmid-map-sequence-id-405780

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  14. pLenti PGK mKate2-OGT 5N5A (Puro)

    Plasmid
    #154293
    Purpose
    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, 5N5A variant, no HCF-1 cleavage)
    Depositor
    Suzanne Walker
    Article
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Lentiviral
    Tags
    2x FLAG and mKate2
    Expression
    Mammalian
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations; HCF-…
    Promoter
    PGK
    Available Since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154293-plasmid-map-sequence-id-307161

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  15. pCJ209

    Plasmid
    #162682
    Purpose
    Lidless variant of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) replacing Gly251:Thr472 of MHETase with the 7-residue loop (Trp185:Phe191, PETase numbering) of PETase , codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1) with the lid removed
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of G251-T472 with W185-F191 from PETa…
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162682-plasmid-map-sequence-id-320836

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  16. pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015

    Plasmid
    #107582
    Purpose
    B. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)
    Depositor
    Paul Freemont
    Article
    Moore et al Proc Natl Acad Sci U S A. 2018 Apr 17. p
    Insert
    GFP+
    Use
    Synthetic Biology; E. coli and bacillus shuttle v…
    Tags
    B. megaterium DSM319 xylA leader sequence (MTSSKI…
    Expression
    Bacterial
    Mutation
    F64L/ S65T/ Q80R/ F99S/ M153T/ V163A
    Promoter
    fba promoter Bacillus megaterium DSM319
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107582-plasmid-map-sequence-id-209150

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  18. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162679-plasmid-map-sequence-id-321456

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  19. pCJ211

    Plasmid
    #162684
    Purpose
    pET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with deletion of the predicted signal peptide (Lys2:Gly19), with C-terminal His tag, codon optimized for expression in E. coli K12.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    Putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) without 19 residue signal peptide
    Tags
    His
    Expression
    Bacterial
    Mutation
    deltaK2:G19; codon optimized for expression in E.…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162684-plasmid-map-sequence-id-321526

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  21. Flag_MmPERK_S503_N1114_pBabePuro

    Plasmid
    #58423
    Purpose
    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant mouse PERK deleted from amino acids M1 to Y502. The remaining amino acids are S503 to N1114.
    Depositor
    David Ron
    Article
    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11
    Insert
    PERK (Eif2ak3 Mouse)
    Use
    Retroviral
    Tags
    FLAG and preprotrypsin signal sequence
    Expression
    Mammalian
    Mutation
    Deleted amino acids M1 to Y502
    Available Since
    Sept. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    58423-plasmid-map-sequence-id-123700

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  22. pABD1031

    Plasmid
    #68004
    Purpose
    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    Tags
    eYFP
    Expression
    Plant
    Promoter
    2X Cauliflower Mosaic Virus 35S promoter
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  23. t1-400

    Plasmid
    #166577
    Purpose
    For expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Article
    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11
    Insert
    Tln1 Head (1-400) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-400 only
    Available Since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166577-plasmid-map-sequence-id-330256

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  24. N229A (EcQ) MetNIQ in pBAD

    Plasmid
    #118257
    Purpose
    N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (N229A) (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala) and…
    Promoter
    None and araBAD Promoter
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    118257-plasmid-map-sequence-id-237124

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  25. N295A (EcN), N229A (EcQ) MetNIQ in pBAD

    Plasmid
    #118581
    Purpose
    N295A Mutation of MetN in Methionine ABC Transporter and N229A Mutation in Methionine Binding Protein (MetQ) for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 229 to Alanine (Ala) and…
    Promoter
    None and araBAD Promotoer
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118581-plasmid-map-sequence-id-228549

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  26. N295A (EcN) MetNIQ in pBAD

    Plasmid
    #118256
    Purpose
    N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Asparagine (Asn) 295 to Alanine (Ala)
    Promoter
    None and araBAD Promotoer
    Available Since
    Nov. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    118256-plasmid-map-sequence-id-228530

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  27. N295A (EcN), Y160A (EcI) MetNIQ in pBAD

    Plasmid
    #118260
    Purpose
    N295A Mutation of MetN and Y160A in MetI in Methionine ABC Transporter for Transport Activity Assay
    Depositor
    Douglas Rees
    Article
    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p
    Inserts
    MetN (N295A) (metN E. coli)
    MetI (Y160A) (metI E. coli)
    MetQ (metQ E. coli)
    Tags
    10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…
    Expression
    Bacterial
    Mutation
    Changed Glutamic Acid (Glu) 295 to Alanine (Ala) …
    Promoter
    None and araBAD Promoter
    Available Since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    118260-plasmid-map-sequence-id-228496

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  28. pAJ1

    Plasmid
    #138379
    Purpose
    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ
    Depositor
    Seth Darst
    Article
    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11
    Inserts
    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…
    Promoter
    T7
    Available Since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138379-plasmid-map-sequence-id-275999

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  30. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 6331 - 6360 of 10278 results