-
Plasmid#146761PurposeMammalian Expression of HsC2orf68-EBMmutDepositorInsertHsC2orf68-EBMmut (C2orf68 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-BTLA (PD-1 ITIM)-mGFP
Plasmid#180818PurposeExpression of human BTLA with immunoreceptor tyrosine-based inhibitory motif (ITIM) from PD-1, fused to mEGFPDepositorInsertBTLA (PD-1 ITIM) (BTLA Human)
UseLentiviralTagsmEGFPExpressionMutationreplaced BTLA-ITIM (IVYASL) with PD-1-ITIM (VDYGE…PromoterSFFVAvailable sinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-F502A_S
Plasmid#147501PurposeMammalian Expression of HsNot2-F502ADepositorInsertHsNot2-F502A (CNOT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_102-330-V5His6_D
Plasmid#146129PurposeInsect Expression of DmEDC3_102-330DepositorInsertDmEDC3_102-330 (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3_441-680_D
Plasmid#146130PurposeInsect Expression of DmEDC3_441-680DepositorInsertDmEDC3_441-680 (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-D61A_D
Plasmid#146131PurposeInsect Expression of DmEDC3-D61ADepositorInsertDmEDC3-D61A (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-D61K_D
Plasmid#146132PurposeInsect Expression of DmEDC3-D61KDepositorInsertDmEDC3-D61K (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmEDC3-N44A_D
Plasmid#146135PurposeInsect Expression of DmEDC3-N44ADepositorInsertDmEDC3-N44A (Edc3 Fly)
UseTagsExpressionInsectMutationone silent mutation A456G and three mutations N16…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCup_C
Plasmid#146064PurposeInsect Expression of DmCupDepositorInsertDmCup (cup Fly)
UseTagsExpressionInsectMutationTwo silent mutations compared to the sequence giv…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B_1-267_C
Plasmid#146065PurposeInsect Expression of DmMe31B_1-267DepositorInsertDmMe31B_1-267 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B_268-459_C
Plasmid#146066PurposeInsect Expression of DmMe31B_268-459DepositorInsertDmMe31B_268-459 (me31B Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2604
Plasmid#175088PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T604A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationS644APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2601
Plasmid#175085PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions S47A, S65A, T75A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationS47A, S65A, T75APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2602
Plasmid#175086PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T424A, T438A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationT424A, T438APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDD2603
Plasmid#175087PurposeMammalian expression of KSHV Orf 50 RTA phosphomutant generated by amino acid substitutions T504A, T508A introduced to WT RTA sequence from Nakamura et al., JVI 2003. 77:4205-20 (PMID: 12634378).DepositorInsertKSHV RTA phospho-mutant (ORF50) (ORF50 Kaposi's sarcoma-associated herpesvirus 8)
UseTagsExpressionMammalianMutationT504A, T508APromoterAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMART-Ala2
Plasmid#71326PurposeNADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli ExpressionDepositorInsertNADPH Alanine Dehydrogenase (ald Synthetic)
UseTagsExpressionBacterialMutationD196A, L197RPromoterinsulated waaHp from E. coliAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD445
Plasmid#168775PurposeExpression of mEos3.2 in MycobacteriumDepositorInsertmEos3.2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbB8k-csg-NbSARS
Plasmid#171703PurposeArabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA is fused to a cross-reactive nanobody targeting the spike protein of betacoronaviruses via a flexible linker.DepositorInsertcsgBACEFG, with nanobody fused to CsgA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFL GST-UBXN2A
Plasmid#169016PurposeExpresses GST-UBXN2A (human) in insect cellsDepositorInsertUBXN2A (UBXN2A Human)
UseTagsGSTExpressionInsectMutationPromoterpolyhedrin promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#797
Plasmid#110916PurposeExpresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neuronsDepositorInsertsunc-129 promoter
STRN-1 (Sentryn)-GFP
unc-54 3' control region with 1 intron just upstream and 1 intron in control region
UseTagsGFP (contains 3 artificial introns)ExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#920
Plasmid#110937PurposeExpresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animalDepositorInsertsmig-13 promoter
INS-22-Emerald
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shSpc25
Plasmid#160960PurposeSpc25 shRNA in pMKO.1 retroviral vectorDepositorInsertSpc25 shRNA (Spc25 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1-shNuf2
Plasmid#160961PurposeNuf2 shRNA in pMKO.1 retroviral vectorDepositorInsertNuf2 shRNA (Nuf2 Mouse)
UseRNAi and RetroviralTagsExpressionMutationPromoterU6Available sinceDec. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Puro shRNA INPP5E KD2
Plasmid#162005PurposeINPP5E shRNADepositorInsertINPP5E (INPP5E Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 Hygro shRNA INPP4B KD2
Plasmid#162003PurposeINPP4B shRNADepositorInsertINPP4B (INPP4B Canis Lupus)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVE2_20_p40S_QfDBD_VP16AD_pFullLength_pcbAB_DsRed
Plasmid#154242PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbABDepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVE2_19_p40S_QfDBD_VP16AD_pFullLength_pcbC_DsRed
Plasmid#154241PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with full lenght promoter pcbCDepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVE2_4_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_5xQUAS(rev)
Plasmid#154237PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS(reversed)+CP_pcbCDepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVE2_12_p40S_QfDBD_VP16AD_5xQUAS_DsRed
Plasmid#154226PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 5xQUAS (no CP control)DepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVE2_1_p40S_QfDBD_VP16AD_cp_pcbC_DsRed_1xQUAS
Plasmid#154221PurposeEncodes p40S:QfDBD-VP16AD-GFP-NLS synthetic transcription factor, activating DsRed-SKL with promoter 1xQUAS+CP_pcbCDepositorInsertp40S:QfDBD-VP16AD-GFP-NLS
UseSynthetic Biology; Fungal expressionTagsGFP-NLS; DsRed-SKLExpressionBacterialMutationPromoterp40S (AN0465 A. nidulans)Available sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only