167,513 results

1. H3K9me3Probe_FullLength

   Plasmid

   #186463

   Purpose

   Expresses GFP tagged H3K9me3 recognition motif in mammalian cells.

   Depositor

   Chongli Yuan

   Article

   Mendonca et al Cytometry A. 2022 Apr;101(4):339-350. do

   Insert

   dimeric CDY1 (CDY1 Human)
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YAP/TAZ tdTomato TEAD Binding Reporter

   Plasmid

   #228554

   Purpose

   Encodes a tdTomato fluorescent protein gene driven by a TEAD binding synthetic promoter. Used to track YAP/TAZ signaling pathway activity.

   Depositor

   Cole DeForest

   Article

   Kopyeva et al Adv Mater. 2024 Sep 6:e2404880. doi: 10.

   Insert

   tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   GTIIC TEAD-Binding Responsive Element

   Available Since

   Feb. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDONR221_SLC6A15

   Plasmid

   #131877

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   Giulio Superti-Furga, RESOLUTE Consortium

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC6A15 (SLC6A15 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 9, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. 764 pME nls-mApple

   Plasmid

   #195962

   Purpose

   Gateway pME fluorophore

   Depositor

   Kristen Kwan, Christian Mosimann

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Insert

   mApple nuclear
   

   Use

   Other

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1-MET-V5/HIS

   Plasmid

   #201982

   Purpose

   expression of human MET receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   MET receptor tyrosine kinase (MET Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YPet-N1

   Plasmid

   #54637

   Purpose

   Localization: N1 Cloning Vector, Excitation: 513, Emission: 527

   Depositor

   Patrick Daugherty, Michael Davidson

   Article

   Nguyen et al Nat Biotechnol. 2005 Mar;23(3):355-60. d

   Type

   Empty backbone

   Tags

   YPet

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pHR_SFFv_Her2-BFP_RHL027

   Plasmid

   #164823

   Purpose

   Lentiviral vector for constitutive expression of HER2+TM(23-675)-BFP

   Depositor

   Wendell Lim

   Article

   Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

   Insert

   Extracellular and transmembrane region of ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Promoter

   SFFV

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70

   Plasmid

   #62897

   Purpose

   Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4-Hsp70
   

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mRuby3-C1

   Plasmid

   #127808

   Purpose

   mRuby3 C1 Cloning vector

   Depositor

   Salvatore Chiantia

   Article

   Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

   Insert

   mRuby3
   

   Expression

   Mammalian

   Available Since

   June 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-hSyn-GRAB_g5-HT3.0 (AAV2)

    Viral Prep

    #208717-AAV2

    Purpose

    Ready-to-use AAV2 particles produced from pAAV-hSyn-GRAB_g5-HT3.0 (#208717). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_g5-HT3.0 plasmid DNA. Synapsin-driven expression of GRAB_g5-HT3.0 sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Yulong Li

    Article

    Deng et al bioRxiv 2023.05.27.542566

    Promoter

    Syn

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

11. pNuc-cis

    Plasmid

    #202796

    Purpose

    RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

    Depositor

    David Edgell

    Article

    Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

    Insert

    TevSpCas9, sgRNA cassette, oriT, CenArsHis
    

    Expression

    Bacterial

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TUBG1_pLX307

    Plasmid

    #98376

    Purpose

    Lentiviral expression of TUBG1

    Depositor

    Sefi Rosenbluh, William Hahn

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    TUBG1 (TUBG1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    E1Fa

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1-Flag-PGC-1alpha1

    Plasmid

    #45501

    Depositor

    Jorge Ruas, Bruce Spiegelman

    Article

    Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

    Insert

    PGC-1alpha Isoform1 (Ppargc1a Mouse)
    

    Tags

    6xHis and Flag

    Expression

    Mammalian

    Mutation

    Q339K relative to NM_008904

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Flag-hCRM1

    Plasmid

    #17647

    Depositor

    Xin Wang

    Article

    Wang et al Nat Cell Biol. 2005 Aug . 7(8):823-30.

    Insert

    CRM1 (XPO1 Human)
    

    Tags

    3x Flag

    Expression

    Mammalian

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pmRFP-LC3

    Plasmid

    #21075

    Purpose

    Mammalian expression of rat LC3 fused to mRFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kimura et al Autophagy. 2007;3(5):452-60.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Available Since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. shh10

    Plasmid

    #64867

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.

    Depositor

    David Schaffer, John Flannery

    Article

    Klimczak et al PLoS One. 2009 Oct 14;4(10):e7467. doi:

    Insert

    shh10 cap
    

    Use

    AAV

    Mutation

    I319V, N451D, D532N

    Available Since

    Aug. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWZL Blast DN E-cadherin

    Plasmid

    #18800

    Depositor

    Bob Weinberg

    Article

    Onder et al Cancer Res. 2008 May 15. 68(10):3645-54.

    Insert

    Dom neg E-cadherin (Cdh1 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    truncated form of E-cadherin (dominant negative) …

    Available Since

    July 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28a MISTIC

    Plasmid

    #85999

    Purpose

    bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag

    Depositor

    Wolfram Antonin

    Article

    Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi:

    Insert

    N/A
    

    Tags

    HIS6 and MISTIC

    Expression

    Bacterial

    Mutation

    codon optimzed for bacterial expression

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-DIO-Ef1a-TVA-2A-N2cG

    Plasmid

    #172360

    Purpose

    Targeting envA-pseodotyped RVdG vectors for specific retrograde labeling specifically from cre-expressing neurons

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    TVA + B19-N2cG
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pVoPo-mCh

    Plasmid

    #187841

    Purpose

    Constitutive mCherry-expression vector for Fusobacterium nucleatum

    Depositor

    Jörg Vogel

    Article

    Ponath et al Proc Natl Acad Sci U S A. 2022 Oct 4;119

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZCS16 (Peft-3::wrmScarlet::tbb-2 3'UTR in pUC19)

    Plasmid

    #154824

    Purpose

    (eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegans

    Depositor

    Patrick Phillips

    Article

    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

    Insert

    Peft-3::wrmScarlet::tbb-2 UTR
    

    Expression

    Worm

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Ef1a-fDIO hChR2(H134R)-EYFP

    Plasmid

    #55639

    Purpose

    Flp-Dependent ChR2-EYFP

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Has Service

    AAV Retrograde

    Insert

    hChR2(H134R)-EYFP
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Aug. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid

    #84243

    Purpose

    Expresses ABA-inducible VPR-Sa dCas9

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …

    Expression

    Mammalian

    Mutation

    D10A, N580A and Synonymous mutation in PYL1 to re…

    Promoter

    CAG and PGK

    Available Since

    Nov. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFPc1 IP3R3 WT

    Plasmid

    #236453

    Purpose

    transient overexpression of IP3R3 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

    Insert

    IP3R3 (Itpr3 Rat)
    

    Expression

    Mammalian

    Available Since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PGL4.11-COL1A1WT

    Plasmid

    #230915

    Purpose

    Expression of human COL1A1 promoter and testing the promoter activity with luciferase assay.

    Depositor

    Avi Rosenberg

    Article

    Yang et al Lab Invest. 2024 May;104(5):100336. doi:

    Insert

    Human COL1A1 promoter (COL1A1 Human)
    

    Use

    Luciferase

    Tags

    luciferase - Luc2p

    Expression

    Bacterial and Mammalian

    Promoter

    COL1A1

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCW57.1-Tet-UPF1WT (pRKB264)

    Plasmid

    #99146

    Purpose

    Inducible lentiviral construct to express FLAG-tagged UPF1wt

    Depositor

    Robert Bradley

    Article

    Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

    Insert

    UPF1 (UPF1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    First 127 codons are optimized to reduce GC conte…

    Promoter

    pTight

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MAC-DPP4

    Plasmid

    #172416

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

    Insert

    Dipeptidyl peptidase 4 (DPP4 Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Nov. 4, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. epi-BE4max

    Plasmid

    #135975

    Purpose

    Expresses AncBE4max, EBNA1 and blasticidin resistence gene; cloning backbone for sgRNA; Contains Epstein-Barr virus oriP replication origin

    Depositor

    Yongming Wang

    Article

    Qi et al Front Cell Dev Biol. 2020 Sep 25;8:59058

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCAGGS_3E5E_eGFP

    Plasmid

    #103054

    Purpose

    Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

    Depositor

    Elke Mühlberger

    Article

    Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

    Insert

    Ebola virus (ebolavirus Zaire, Mayinga) minigenome
    

    Tags

    3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMD1 anti-GFP nanobody-Tn5

    Plasmid

    #215567

    Purpose

    Expresses anti-GFP nanobody-Tn5 fusion in bacteria with an N-terminal 3xFlag tag and a C-terminal intein-CBD-intein tag

    Depositor

    Isabelle Mansuy

    Article

    Maria A. et al bioRxiv 2024.07.12.603224

    Insert

    anti-GFP nanobody-Tn5 fusion
    

    Tags

    3xFlag and intein-CBD

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLXSN16E6E7

    Plasmid

    #52394

    Purpose

    The plasmid, pLXSN16E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV16 E6 and E7 genes.

    Depositor

    Denise Galloway

    Article

    Halbert et al J Virol. 1991 Jan;65(1):473-8.

    Insert

    HPV16 E6E7
    

    Use

    Retroviral

    Available Since

    May 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

    Plasmid

    #122151

    Purpose

    Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    dCas9 fused to SunTag(24x)
    

    Use

    Lentiviral

    Tags

    2xNLS-P2A-BFP and NLS

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SIRT3L-12

    Plasmid

    #13736

    Depositor

    John Denu

    Article

    Hallows et al Proc Natl Acad Sci U S A. 2006 Jul 5. 10

    Insert

    SIRT3 (SIRT3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pAAV-S5E2-dTom-nlsdTom (AAV9)

    Viral Prep

    #135630-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-S5E2-dTom-nlsdTom (#135630). In addition to the viral particles, you will also receive purified pAAV-S5E2-dTom-nlsdTom plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jordane Dimidschstein

    Article

    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

    Tags

    dTomato

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

40. mAG-GAL3

    Plasmid

    #62734

    Purpose

    Mammalian vesicle leakage reporter

    Depositor

    Niels Geijsen

    Article

    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

    Insert

    mAG-Galectin 3
    

    Use

    Lentiviral

    Tags

    mAzami-Green

    Expression

    Mammalian

    Promoter

    EF1-Alpha

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pX330-PITCh-TUBA1B

    Plasmid

    #207763

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839) (AAV PHP.eB)

    Viral Prep

    #163509-PHPeB

    Purpose

    Ready-to-use AAV PHP.eB particles produced from AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839) (#163509). In addition to the viral particles, you will also receive purified AiP11839-pAAV-hSyn1-SYFP2-10aa-H2B-WPRE3-BGHpA (Alias: CN1839) plasmid DNA. hSyn1-driven expression for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Jonathan Ting, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Promoter

    Syn

    Tags

    SYFP2

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

43. U6-sgRNA(HEK3)-CMV-SpCas9(D10A)-P2A-EGFP

    Plasmid

    #221233

    Purpose

    Express sgRNA targeting HEK3 loci with nCas9(D10A)

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Insert

    SpCas9(D10A)-P2A-EGFP
    

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAV_BiVIPe4_ChR2_mCherry

    Plasmid

    #213856

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pHAGE-TO-dCas9-3XmCherry

    Plasmid

    #64108

    Purpose

    Expression of Sp dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pRSFDuet-1(nsp8-nsp7)(nsp12)

    Plasmid

    #165451

    Purpose

    Expresses the RNA Dependent RNA polymerase complex of SARS-CoV-2 in Escherichia Coli

    Depositor

    Marc Delarue

    Article

    Madru et al PLoS One. 2021 Apr 29;16(4):e0250610. do

    Inserts

    nsp12
    nsp8
    nsp7
    

    Tags

    14Histidine-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pT7-NSP4SA11

    Plasmid

    #89171

    Purpose

    Express Rotavirus SA11 NSP4 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP4
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pVAX1/mTyr-Cre

    Plasmid

    #99249

    Purpose

    Expresses Cre recombinase under tyrosinase promoter

    Depositor

    Christian Blank

    Article

    Deken et al Int J Mol Sci. 2016 Dec 20;17(12). pii:

    Inserts

    Cre recombinase
    mTyr enh/prom
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. mCh-KIF5B*-strep

    Plasmid

    #120164

    Purpose

    Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

    Depositor

    Juan Bonifacino

    Article

    Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

    Insert

    kinesin family member 5B (Kif5b Mouse)
    

    Tags

    Streptavidin and mCherry

    Expression

    Mammalian

    Mutation

    Truncated KIF5B: 1-555 aa

    Promoter

    CMV

    Available Since

    July 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pLCKO2

    Plasmid

    #125518

    Purpose

    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

    Depositor

    Jason Moffat

    Article

    Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge