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Showing: 10551 - 10600 of 160217 results

  5. pD649-HAsp-MERTK-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156878
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    MERTK (MERTK Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    156878-plasmid-map-sequence-id-382010

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  12. pX330 _CRISPIE donor B7

    Plasmid
    #172848
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172848-plasmid-map-sequence-id-341024

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  14. TFORF1344

    Plasmid
    #143792
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    FEZF2 (FEZF2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    143792-plasmid-map-sequence-id-396243

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  16. EMMA toolkit

    Plasmid Kit
    #1000000119
    Purpose
    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production
    Depositor
    Yizhi Cai
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-CAG-RdLight1 (AAV9)

    Viral Prep
    #125707-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-CAG-RdLight1 (#125707). In addition to the viral particles, you will also receive purified pAAV-CAG-RdLight1 plasmid DNA. CAG-driven expression of red genetically encoded dopamine sensor RdLight1. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Lin Tian
    Article
    Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.
    Promoter
    CAG
    Tags
    None
    Available since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only

  21. PB-CA sfGFP

    Plasmid
    #159094
    Purpose
    PB-CA sfGFP piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    159094-plasmid-map-sequence-id-316224

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  29. pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV5)

    Viral Prep
    #55645-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent channelrhodopsin-EYFP expression for optogenetic activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Article
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    Syn
    Tags
    EYFP (Cre- and Flp-dependent)
    Available since
    Dec. 2, 2019
    Availability
    Academic Institutions and Nonprofits only

  31. 2xMARS

    Plasmid
    #205233
    Purpose
    Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-iin mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    two repeats of PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    Use
    Tags
    Nuclear Export Sequence and mScarlet-i
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205233-plasmid-map-sequence-id-404744

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Showing: 10551 - 10600 of 160217 results