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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,817 results for: cdna

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Showing: 4261 - 4280 of 13817 results

  3. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid
    #140984
    Purpose
    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Insert
    GAlpha15-RLuc8 (GNA15 Human)
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    June 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140984-plasmid-map-sequence-id-300775

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  5. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:
    Insert
    SPRTN (SPRTN Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110215-plasmid-map-sequence-id-210950

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  8. pcDNA3.1-SARS2-Spike-HiBiT

    Plasmid
    #191212
    Purpose
    Transient expression in mammalian cells of SARS-CoV-2 (A1) spike protein tagged on the C-terminus with HiBiT, the high affinity small fragment of split nanoluciferase assay
    Depositor
    Mohan Babu
    Article
    Broderick et al Nat Commun. 2025 Jul 1;16(1):5784. doi:
    Insert
    SARS-CoV-2 spike_WT (S SARS CoV-2)
    Tags
    HiBiT (high affinity small nanoluciferase fragmen…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    191212-plasmid-map-sequence-id-437009

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  10. pcDNA3.1+CMV-Ins-GLuc

    Plasmid
    #89929
    Purpose
    Expresses Insulin-GLuc from the CMV promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Synthetic, Human)
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    CMV
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89929-plasmid-map-sequence-id-255704

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  19. pcDNA3.1+ eiF4A1 myc HIS

    Plasmid
    #71657
    Purpose
    mammalian expression of eiF4A1
    Depositor
    Gideon Dreyfuss
    Insert
    eiF4A1 (EIF4A1 Human)
    Tags
    HIS and myc
    Expression
    Mammalian
    Mutation
    aa403 from Ala to Arg
    Promoter
    CMV
    Available Since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71657-plasmid-map-sequence-id-422687

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Showing: 4261 - 4280 of 13817 results