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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,885 results for: Coli

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Showing: 4281 - 4300 of 9885 results

  2. pTU2S-b (colE1 origin)

    Plasmid
    #74092
    Purpose
    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication
    Depositor
    Paul Freemont
    Article
    Moore et al ACS Synth Biol. 2016 May 2.
    Insert
    Golden Gate destination vector - Level 2, 3 TU's with secondary module and colE1 origin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    None
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    74092-plasmid-map-sequence-id-141928

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  10. HpMscS

    Plasmid
    #78310
    Purpose
    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag
    Depositor
    Douglas Rees
    Article
    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:
    Insert
    Mechanosensitive channel of small conductance
    Tags
    6x His Tag and FLAG Tag
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    June 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    78310-plasmid-map-sequence-id-151301

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  11. pIBA2-SpyTag-MBP

    Plasmid
    #107422
    Purpose
    Control construct for SpyCatcher experiments. Encodes MBP with N-terminal SpyTag, including OmpA signal peptide for periplasmic targeting.
    Depositor
    Jack Leo
    Article
    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/
    Insert
    Maltose-binding protein (MalE)
    Tags
    OmpA signal peptide, SpyTag, and StrepII
    Expression
    Bacterial
    Promoter
    Tet
    Available Since
    Jan. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    107422-plasmid-map-sequence-id-212955

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  12. pSLS.546

    Plasmid
    #184963
    Purpose
    Test effect of a1/a2 length on editing priB using retron recombineering
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
    Expression
    Bacterial
    Mutation
    priB donor G1069A, a1/a2 length extended to 22 bp
    Promoter
    T7/lac
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184963-plasmid-map-sequence-id-376688

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  14. pRB113.2

    Plasmid
    #181948
    Purpose
    aTc-inducible expression of NarL with C-terminal mNeonGreen fusion. Also contains mCherry under NarL-controlled promoter PdcuS
    Depositor
    Jeffrey Tabor
    Article
    Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11
    Insert
    NarL-mNG; mCherry; tetR (AX04_RS23485, narL Synthetic, narL - E. coli)
    Tags
    mNeonGreen
    Expression
    Bacterial
    Promoter
    NarL-mNG-PLtetO-1; mCherry-PdcuS; tetR-J23106
    Available Since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    181948-plasmid-map-sequence-id-356574

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Showing: 4281 - 4300 of 9885 results