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pN_35S/mCitrine/P_UBQ10/Derlin1-mOrange2

Plasmid

#118000

Purpose

Co-expression of mCitrine under the control of the CaMV 35S promoter and Derlin1 fused to mOrange2 (an ER-targeting marker protein) under the control of the A. thaliana ubiquitin-10 promoter.

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

Derlin1-mOrange2

Use

Plant/bacteria binary vector

Expression

Plant

Promoter

Ubiquitin-10

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_KDM6A_1

Plasmid

#86307

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRetro-XT-PAK2-CFP(puro)-T402A

Plasmid

#81179

Purpose

Retroviral vector for expression of kinase inactive PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Mutation

T402A

Promoter

P-tight TRE

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_ARID5B

Plasmid

#86288

Purpose

Donor vector for 3' FLAG tag of human ARID5B

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ARID5B homology arms (ARID5B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMX GFP cdc42 Q61L

Plasmid

#14568

Depositor

Joan Brugge

Article

Hall et al Immunity. 2006 Mar . 24(3):305-16.

Insert

cdc42 Q61L (CDC42 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Available Since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
V5-bArr1

Plasmid

#201491

Purpose

Expression of the beta-arrestin1 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

V5-bArr1 (ARRB1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]

Plasmid

#168170

Purpose

Expression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegans

Depositor

Alexander Gottschalk

Article

Henss et al Br J Pharmacol. 2021 Mar 18. doi: 10.111

Insert

YFP-BeCyclOp(A-2x) (codon optimized for C. elegans)

Tags

YFP

Expression

Worm

Mutation

E497K and C566D

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-Epsin 2

Plasmid

#22241

Depositor

Pietro De Camilli

Article

Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

Insert

Epsin 2 (EPN2 Human)

Tags

HA

Expression

Bacterial and Mammalian

Available Since

Oct. 9, 2009

Availability

Academic Institutions and Nonprofits only
IG510: pMVP (R4-R3) human PDX1

Plasmid

#121744

Purpose

pMVP R4-R3 entry plasmid, contains human PDX1 for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Human PDX1 (PDX1 Human)

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRetro-XT-PAK2-CFP(puro)-L107F

Plasmid

#81180

Purpose

Retroviral vector for expression of kinase active PAK2 with a CFP fusion

Depositor

Angeliki Malliri

Article

Whalley et al Nat Commun. 2015 Jun 16;6:7437. doi: 10.

Insert

p21 (RAC1) activated kinase 2 (PAK2 Human)

Use

Retroviral

Tags

CFP

Mutation

L107F

Promoter

P-tight TRE

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pNH12 (pmyo-2::MacQ::mCitrine)

Plasmid

#130274

Purpose

Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

MacQ::mCitrine

Tags

mCitrine

Expression

Worm

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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PX458_KDM6A_2

Plasmid

#86308

Purpose

Encodes gRNA for 3' target of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM6A (KDM6A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_MIER3

Plasmid

#86274

Purpose

Donor vector for 3' FLAG tag of human MIER3

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MIER3 homology arms (MIER3 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_FOXP1

Plasmid

#86261

Purpose

Donor vector for 3' FLAG tag of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXP1 homology arms (FOXP1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMCL-HA-MAPKK2-KW71[delta(48-55)/S222D/S226D]

Plasmid

#40821

Depositor

Natalie Ahn

Article

Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

Insert

MAP2K2 (MAP2K2 Human)

Tags

HA

Expression

Mammalian

Mutation

delta(48-55) + S222D + S226D

Promoter

CMV

Available Since

Nov. 14, 2012

Availability

Academic Institutions and Nonprofits only
pUBQ10:AtFLS2

Plasmid

#202186

Purpose

Express Arabidopsis thaliana FLS2 gene under UBQ10 promoter

Depositor

Gitta Coaker

Article

Trinh et al Plant Physiol. 2023 May 5:kiad263. doi:

Insert

Flagellin sensing 2 (FLS2 Mustard Weed)

Tags

HA

Expression

Plant

Promoter

AtUBQ10

Available Since

Sept. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFETCh_KAT7

Plasmid

#86266

Purpose

Donor vector for 3' FLAG tag of human KAT7

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KAT7 homology arms (KAT7 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_FOXP1_1

Plasmid

#86299

Purpose

Encodes gRNA for 3' target of human FOXP1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against FOXP1 (FOXP1 Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_KDM6A

Plasmid

#86265

Purpose

Donor vector for 3' FLAG tag of human KDM6A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

KDM6A homology arms (KDM6A Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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KJ901: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS

Plasmid

#121835

Purpose

pMAGIC R4-R3 entry plasmid, contains 2x NLS scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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