We narrowed to 90,451 results for: des.1

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YFP-PBD

Plasmid

#11407

Depositor

Joel Swanson

Article

Hoppe et al Mol Biol Cell 2004 Aug;15(8):3509-19. Ep

Insert

p21-activated kinase 1 PBD (PAK1 Human)

Tags

YFP

Expression

Mammalian

Available Since

Feb. 21, 2007

Availability

Academic Institutions and Nonprofits only
pEJS1099: Dual-sgRNA.Design 4

Plasmid

#159537

Purpose

Delivery of dual sgRNA cassettes and Nme2Cas9 in a single AAV vector

Depositor

Erik Sontheimer

Article

Ibraheim et al Nat Commun. 2021 Nov 1;12(1):6267. doi:

Insert

Nme2Cas9 with two guide RNA cassettes

Use

AAV, CRISPR, and Mouse Targeting

Tags

NLS

Expression

Mammalian

Promoter

U1a

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK4(ala)

Plasmid

#15314

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1995 Jul 21. 269(5222):403-7.

Insert

MKK4(ala) (MAP2K4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ser-220Ala Thr-224Ala

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only
pMSCVneo-DEST

Plasmid

#119746

Purpose

Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

Depositor

Andrew Jackson, Martin Reijns

Article

Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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MD-2

Plasmid

#20864

Depositor

Jie-Oh Lee

Article

Park et al Nature. 2009 Apr 30;458(7242):1191-5. do

Insert

Myeloid differentiation factor 2 (LY96 Human)

Tags

protein A tag

Expression

Insect

Mutation

contains amino acids 19–160

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pBp-FGFR2b-WT

Plasmid

#45698

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pMG36E

Plasmid

#133853

Purpose

Expression vector

Depositor

Jan Kok, Gerard Venema

Article

van de Guchte et al Appl Environ Microbiol. 1989 Jan;55(1):2

Type

Empty backbone

Expression

Bacterial

Promoter

P32

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-NTRK1

Plasmid

#23891

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

NTRK1 (NTRK1 Human)

Use

Gateway Cloning

Mutation

V263L relative to NM_001012331

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/FLAG/HA-DEST PABPC4

Plasmid

#19882

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Poly(A) binding protein, cytoplasmic 4 (PABPC4 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3A-BE3

Plasmid

#113410

Purpose

Expresses hA3A-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3A-BE3 (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-5ptaseOCRL

Plasmid

#66836

Purpose

Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

5ptaseOCRL (OCRL Human)

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pRcCMV myc-Plk1 T210D (Nigg LA183)

Plasmid

#41158

Depositor

Erich Nigg

Article

Smits et al Nat Cell Biol. 2000 Sep;2(9):672-6.

Insert

Plk1 (PLK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

T210D

Promoter

CMV

Available Since

Feb. 6, 2013

Availability

Academic Institutions and Nonprofits only
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64219

Purpose

Expression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked via T2A to BFP linked to the Ad4 E1B55K gene via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA targeting ROSA26-1

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pJJiaDEST-APEX2

Plasmid

#149517

Purpose

N-termial APEX2 tag. Gateway destination vector for mammanlian expression

Depositor

Vojo Deretic

Article

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Type

Empty backbone

Tags

FLAG-APEX2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDest490-OPN-b

Plasmid

#17591

Depositor

Xin Wang

Article

Takafuji et al Oncogene. 2007 Sep 27. 26(44):6361-71.

Insert

OPN-b (SPP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Base pairs 1–175, 217–942; See Depositor Comments

Available Since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pRK5-p18-FLAG

Plasmid

#42331

Depositor

David Sabatini

Article

Bar-Peled et al Cell. 2012 Sep 14;150(6):1196-208. doi:

Insert

p18 (LAMTOR1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV, SP6

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX TIF1gamma

Plasmid

#15734

Depositor

Joan Massague

Article

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

TIF1 gamma (TRIM33 Human)

Tags

GST

Expression

Bacterial

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pSUMO2

Plasmid

#52259

Purpose

Bacterial expression of human 6His-tagged SUMO2, SUMO-E1 and SUMO-E2

Depositor

Primo Schaer

Article

Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

Inserts

SUMO-2 (SUMO2 Human)
UBE2l (UBE2I Human)
SAE-2 (UBA2 Human)
SAE-1 (SAE1 Human)

Tags

6His-tag

Expression

Bacterial

Mutation

C-terminal gly-gly

Promoter

T7 and rbs

Available Since

July 10, 2014

Availability

Academic Institutions and Nonprofits only

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pDEST51-LRRK2-R1441C

Plasmid

#25081

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Use

Gateway Cloning

Tags

6XHis and V5

Expression

Mammalian

Mutation

Arginine 1441 mutated to Cysteine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pCFJ2474 - pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Plasmid

#159816

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Psmu-2 | cas9(PATCs) | gpd-2 tagRFP-T(myr, patcs) smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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