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Showing: 6451 - 6480 of 32840 results

  2. pVRC8400-SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D-AVI

    Plasmid
    #176332
    Purpose
    Expresses SARS-CoV-2 S2P protein from the B.1.617 variant containing G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D mutations with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-B.1.617-G142D-E154K-L452R-E484Q-D614G-P681R-Q1071H-H1101D (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    176332-plasmid-map-sequence-id-347141

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  4. TRUPATH Triple Gai2

    Plasmid
    #196049
    Purpose
    Encodes a G alpha subunit (GNAl2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphai2-RLuc8 (GNAI2 Human)
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196049-plasmid-map-sequence-id-385863

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  5. TRUPATH Triple Ga11

    Plasmid
    #196059
    Purpose
    Encodes a G alpha subunit (GNA11) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha11-RLuc8 (GNA11 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    196059-plasmid-map-sequence-id-386126

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  6. TRUPATH Triple Ga15

    Plasmid
    #196060
    Purpose
    Encodes a G alpha subunit (GNA15) with RLuc8, a G gamma subunit (GNG13) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlpha15-RLuc8 (GNA15 Human)
    GGamma13-GFP2 (GNG13 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    Feb. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    196060-plasmid-map-sequence-id-385870

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  7. TRUPATH Triple Gagust

    Plasmid
    #196054
    Purpose
    Encodes a G alpha subunit (GNAT3) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaGustducin-RLuc8 (GNAT3 Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196054-plasmid-map-sequence-id-393090

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  8. TRUPATH Triple GaZ

    Plasmid
    #196053
    Purpose
    Encodes a G alpha subunit (GNAZ) with RLuc8, a G gamma subunit (GNG1) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins
    Depositor
    Justin English
    Article
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Inserts
    GAlphaz-RLuc8 (GNAZ Human)
    GGamma1-GFP2 (GNGT1 Human)
    GBeta3 (GNB3 Human)
    Use
    Luciferase
    Tags
    GFP2 and GSAG linker
    Expression
    Mammalian
    Mutation
    RLuc8 and flanking SGGGGS linkers have been inser…
    Promoter
    CMV
    Available Since
    March 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    196053-plasmid-map-sequence-id-387469

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  9. pVRC8400-SARS-CoV-2-S2P-XBB.1.5-AVI

    Plasmid
    #198465
    Purpose
    Expresses SARS-CoV-2 S2P protein from the XBB.1.5 variant with single chain Fc, HRV3C protease cleavage site, and AVI tag
    Depositor
    Peter Kwong
    Article
    Teng et al PLoS One. 2022 May 24;17(5):e0268767. do
    Insert
    SARS-CoV-2-S2P-XBB.1.5 (S )
    Tags
    AVI, HRV3C, and scFc
    Expression
    Mammalian
    Mutation
    T19I, L24-, P25-, P26-, A27S, V83A, G142D, Y144-,…
    Available Since
    March 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    198465-plasmid-map-sequence-id-388112

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  10. AAV:ITR-U6-sgRNA(Kras)-U6-sgRNA(p53)-U6-sgRNA(Lkb1)-pEFS-Rluc-2A-Cre-shortPA-KrasG12D_HDRdonor-ITR (AAV-KPL)

    Plasmid
    #60224
    Purpose
    Expresses Cre recombinase and Renilla luciferase from the EFS promoter and three U6-driven sgRNAs targeting Kras, p53, and Lkb1. Contains KrasG12D HDR donor. AAV backbone.
    Depositor
    Feng Zhang
    Article
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Renilla luciferase
    Cre recombinase
    KrasG12D HDR donor (Kras Mouse)
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA and Rluc-P2A
    Expression
    Mammalian
    Promoter
    EFS, No promoter, and U6
    Available Since
    Oct. 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    60224-plasmid-map-sequence-id-418949

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  13. PermaPhos Kit

    Plasmid Kit
    #1000000226
    Purpose
    This kit is used for expressing recombinant proteins with site-specific, non-hydrolyzable phosphoserine (nhpSer) in an engineered self-sufficient E. coli genetic code expansion (GCE) expression system
    Depositor
    Ryan Mehl
    Available Since
    Sept. 12, 2023
    Availability
    Academic Institutions and Nonprofits only

  14. NT-CRISPR plasmid collection

    Plasmid Kit
    #1000000189
    Purpose
    The NT-CRISPR plasmid collection contains all plasmids to perform genome engineering in Vibrio natriegens using the NT-CRISPR method.
    Depositor
    Anke Becker
    Available Since
    May 9, 2022
    Availability
    Academic Institutions and Nonprofits only

  15. pVSVdG-4BFP2

    Plasmid
    #64101
    Purpose
    Expresses mTagBFP2
    Depositor
    Ian Wickersham
    Insert
    mTagBFP2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64101-plasmid-map-sequence-id-109498

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  16. TALE Toolbox

    Plasmid Kit
    #1000000019
    Purpose
    Provides a complete set of plasmids for building your own TALE nucleases (TALENs) and transcription factors (TALE-TFs) for genome editing and transcription modulation applications.
    Depositor
    Feng Zhang
    Available Since
    Dec. 16, 2011
    Availability
    Academic Institutions and Nonprofits only

  17. pCAG-T7pol

    Plasmid
    #59926
    Purpose
    Expression vector for T7 RNA polymerase gene
    Depositor
    Ian Wickersham
    Insert
    T7 RNA polymerase
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    59926-plasmid-map-sequence-id-418961

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  18. Rinehart & Söll Phosphoprotein Synthesis Kit

    Plasmid Kit
    #1000000020
    Purpose
    Allows Sep incorporation (directed by UAG) into a protein by an E. coli strain that harbors a Sep-accepting tRNA (tRNASep), its cognate Sep–tRNA synthetase (SepRS), and an engineered EF-Tu (EF-Sep).
    Depositor
    Jesse Rinehart, Dieter Söll
    Available Since
    March 1, 2012
    Availability
    Academic Institutions and Nonprofits only

  19. pRVdG-4BFP2

    Plasmid
    #52490
    Purpose
    Expresses mTagBFP2
    Depositor
    Ian Wickersham
    Insert
    mTagBFP2
    Use
    Rabies virus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    52490-plasmid-map-sequence-id-81390

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  20. pRVdG-4GCaMP6s

    Plasmid
    #52497
    Purpose
    Expresses GCaMP6s
    Depositor
    Ian Wickersham
    Insert
    GCaMP6s
    Use
    Rabies virus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    52497-plasmid-map-sequence-id-81433

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  21. Denv2_GU289914.1_NS2B

    Plasmid
    #234401
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    NS2B_DENV2
    Tags
    MGSSHHHHHHSSGLVPRGS
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234401-plasmid-map-sequence-id-470061

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  22. Denv4_KJ160504.1_NS2B

    Plasmid
    #234402
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    NS2B_DENV4
    Tags
    MGSSHHHHHHSSGLVPRGS
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    234402-plasmid-map-sequence-id-469864

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  23. pCAG-B19N

    Plasmid
    #59924
    Purpose
    Expression vector for Rabies virus SAD B19 nucleoprotein gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus nucleoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    59924-plasmid-map-sequence-id-94984

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  24. pCAG-B19P

    Plasmid
    #59925
    Purpose
    Expression vector for Rabies virus SAD B19 phosphoprotein gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus phosphoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    59925-plasmid-map-sequence-id-94990

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  25. pCAG-B19G

    Plasmid
    #59921
    Purpose
    Expression vector for Rabies virus SAD B19 glycoprotein gene
    Depositor
    Ian Wickersham
    Insert
    Rabies virus glycoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    59921-plasmid-map-sequence-id-94977

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  26. pOpen-Bstpol

    Plasmid
    #165543
    Purpose
    Full length DNA polymerase from Bacillus stearothermophilus.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bst DNA Polymerase, Full Length
    Use
    Synthetic Biology
    Available Since
    Aug. 9, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165543-plasmid-map-sequence-id-339279

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  27. pAAV-CAG-FLEX-splitTVA800

    Plasmid
    #59330
    Purpose
    Expresses splitTVA800
    Depositor
    Ian Wickersham
    Insert
    splitTVA800
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    59330-plasmid-map-sequence-id-126570

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  28. pAAV-CAG-FLEX-splitTVA950

    Plasmid
    #59329
    Purpose
    Expresses splitTVA950
    Depositor
    Ian Wickersham
    Insert
    splitTVA950
    Use
    AAV and Cre/Lox
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Oct. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    59329-plasmid-map-sequence-id-92025

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  29. pAIP4_4REH

    Plasmid
    #234195
    Purpose
    Heterologous protein expression of major latex-like protein in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4REH
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234195-plasmid-map-sequence-id-464160

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  30. pCAG-VSVG

    Plasmid
    #64084
    Purpose
    Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene
    Depositor
    Ian Wickersham
    Insert
    VSV glycoprotein
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    64084-plasmid-map-sequence-id-107221

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Showing: 6451 - 6480 of 32840 results